Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1639 and GFF1640 overlap by 4 nucleotides GFF1640 and GFF1641 overlap by 1 nucleotides
GFF1639 - Polyketide synthase modules and related proteins, at 268,110 to 271,196
GFF1639
GFF1640 - Polyketide synthase modules and related proteins, at 271,193 to 278,275
GFF1640
GFF1641 - Polyketide synthase modules and related proteins, at 278,275 to 283,587
GFF1641
Position (kb) 271
272
273
274
275
276
277
278
279 Strain fitness (log2 ratio) -2
-1
0
1 at 270.635 kb on - strand, within GFF1639 at 270.700 kb on + strand, within GFF1639 at 270.743 kb on - strand, within GFF1639 at 271.309 kb on - strand at 271.449 kb on + strand at 271.695 kb on + strand at 271.696 kb on - strand at 271.743 kb on + strand at 271.743 kb on + strand at 271.744 kb on - strand at 271.932 kb on + strand, within GFF1640 at 271.933 kb on - strand, within GFF1640 at 271.948 kb on - strand, within GFF1640 at 271.948 kb on - strand, within GFF1640 at 271.948 kb on - strand, within GFF1640 at 271.995 kb on + strand, within GFF1640 at 272.103 kb on + strand, within GFF1640 at 272.437 kb on - strand, within GFF1640 at 272.451 kb on + strand, within GFF1640 at 272.451 kb on + strand, within GFF1640 at 272.914 kb on + strand, within GFF1640 at 273.058 kb on - strand, within GFF1640 at 273.115 kb on - strand, within GFF1640 at 273.126 kb on + strand, within GFF1640 at 273.126 kb on + strand, within GFF1640 at 273.135 kb on + strand, within GFF1640 at 273.135 kb on + strand, within GFF1640 at 273.135 kb on + strand, within GFF1640 at 273.136 kb on - strand, within GFF1640 at 273.190 kb on - strand, within GFF1640 at 273.807 kb on + strand, within GFF1640 at 273.807 kb on + strand, within GFF1640 at 273.807 kb on + strand, within GFF1640 at 274.299 kb on + strand, within GFF1640 at 274.299 kb on + strand, within GFF1640 at 274.788 kb on + strand, within GFF1640 at 274.789 kb on - strand, within GFF1640 at 274.789 kb on - strand, within GFF1640 at 274.845 kb on + strand, within GFF1640 at 274.845 kb on + strand, within GFF1640 at 274.845 kb on + strand, within GFF1640 at 274.846 kb on - strand, within GFF1640 at 275.499 kb on - strand, within GFF1640 at 275.499 kb on - strand, within GFF1640 at 275.529 kb on + strand, within GFF1640 at 275.529 kb on + strand, within GFF1640 at 275.529 kb on + strand, within GFF1640 at 275.530 kb on - strand, within GFF1640 at 275.530 kb on - strand, within GFF1640 at 275.530 kb on - strand, within GFF1640 at 275.530 kb on - strand, within GFF1640 at 276.342 kb on + strand, within GFF1640 at 276.342 kb on + strand, within GFF1640 at 276.414 kb on + strand, within GFF1640 at 276.415 kb on - strand, within GFF1640 at 277.050 kb on + strand, within GFF1640 at 277.050 kb on + strand, within GFF1640 at 277.051 kb on - strand, within GFF1640 at 277.051 kb on - strand, within GFF1640 at 277.593 kb on + strand at 277.755 kb on + strand at 277.756 kb on - strand at 277.756 kb on - strand at 277.869 kb on + strand at 278.074 kb on - strand at 278.074 kb on - strand at 278.637 kb on - strand at 278.744 kb on + strand at 278.744 kb on + strand at 278.744 kb on + strand at 278.744 kb on + strand at 278.745 kb on - strand at 278.745 kb on - strand at 278.745 kb on - strand at 278.819 kb on + strand, within GFF1641 at 278.820 kb on - strand, within GFF1641 at 278.820 kb on - strand, within GFF1641 at 278.820 kb on - strand, within GFF1641 at 278.967 kb on - strand, within GFF1641 at 279.086 kb on + strand, within GFF1641 at 279.116 kb on + strand, within GFF1641 at 279.116 kb on + strand, within GFF1641 at 279.117 kb on - strand, within GFF1641 at 279.209 kb on + strand, within GFF1641 at 279.209 kb on + strand, within GFF1641 at 279.210 kb on - strand, within GFF1641 at 279.210 kb on - strand, within GFF1641
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 270,635 - GFF1639 0.82 +0.6 270,700 + GFF1639 0.84 -0.7 270,743 - GFF1639 0.85 -0.2 271,309 - -0.8 271,449 + -0.5 271,695 + +0.8 271,696 - -1.9 271,743 + +0.1 271,743 + -0.1 271,744 - -0.4 271,932 + GFF1640 0.10 -0.2 271,933 - GFF1640 0.10 -0.2 271,948 - GFF1640 0.11 -1.2 271,948 - GFF1640 0.11 -0.5 271,948 - GFF1640 0.11 +0.2 271,995 + GFF1640 0.11 -0.7 272,103 + GFF1640 0.13 +0.2 272,437 - GFF1640 0.18 -1.5 272,451 + GFF1640 0.18 +1.6 272,451 + GFF1640 0.18 -0.3 272,914 + GFF1640 0.24 -0.2 273,058 - GFF1640 0.26 +0.3 273,115 - GFF1640 0.27 -0.3 273,126 + GFF1640 0.27 -1.2 273,126 + GFF1640 0.27 +0.2 273,135 + GFF1640 0.27 +0.3 273,135 + GFF1640 0.27 +0.1 273,135 + GFF1640 0.27 +0.8 273,136 - GFF1640 0.27 -0.4 273,190 - GFF1640 0.28 -1.6 273,807 + GFF1640 0.37 -0.8 273,807 + GFF1640 0.37 -0.1 273,807 + GFF1640 0.37 -0.1 274,299 + GFF1640 0.44 +0.4 274,299 + GFF1640 0.44 -1.5 274,788 + GFF1640 0.51 -1.4 274,789 - GFF1640 0.51 -1.6 274,789 - GFF1640 0.51 +0.3 274,845 + GFF1640 0.52 -0.0 274,845 + GFF1640 0.52 +0.0 274,845 + GFF1640 0.52 +0.1 274,846 - GFF1640 0.52 +0.3 275,499 - GFF1640 0.61 +0.2 275,499 - GFF1640 0.61 -0.3 275,529 + GFF1640 0.61 +0.0 275,529 + GFF1640 0.61 +0.2 275,529 + GFF1640 0.61 +0.1 275,530 - GFF1640 0.61 -0.2 275,530 - GFF1640 0.61 -0.1 275,530 - GFF1640 0.61 -0.5 275,530 - GFF1640 0.61 -0.2 276,342 + GFF1640 0.73 -0.4 276,342 + GFF1640 0.73 -0.2 276,414 + GFF1640 0.74 -1.3 276,415 - GFF1640 0.74 +1.2 277,050 + GFF1640 0.83 +1.5 277,050 + GFF1640 0.83 +0.8 277,051 - GFF1640 0.83 +0.4 277,051 - GFF1640 0.83 -1.5 277,593 + -1.0 277,755 + -0.7 277,756 - -0.3 277,756 - -0.3 277,869 + -0.2 278,074 - +1.5 278,074 - +0.0 278,637 - +0.1 278,744 + -0.4 278,744 + +0.2 278,744 + -0.8 278,744 + -2.1 278,745 - +0.3 278,745 - +0.1 278,745 - +0.1 278,819 + GFF1641 0.10 -0.8 278,820 - GFF1641 0.10 -2.0 278,820 - GFF1641 0.10 -0.3 278,820 - GFF1641 0.10 -2.1 278,967 - GFF1641 0.13 -1.1 279,086 + GFF1641 0.15 -0.5 279,116 + GFF1641 0.16 +0.8 279,116 + GFF1641 0.16 -0.6 279,117 - GFF1641 0.16 +0.2 279,209 + GFF1641 0.18 -2.1 279,209 + GFF1641 0.18 -0.1 279,210 - GFF1641 0.18 -0.8 279,210 - GFF1641 0.18 +0.8
Or see this region's nucleotide sequence