Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1480 and GFF1481 are separated by 66 nucleotides GFF1481 and GFF1482 overlap by 4 nucleotides GFF1482 and GFF1483 overlap by 13 nucleotides
GFF1480 - Choline-sulfatase (EC 3.1.6.6), at 72,040 to 73,563
GFF1480
GFF1481 - L-carnitine dehydratase/bile acid-inducible protein F, at 73,630 to 74,874
GFF1481
GFF1482 - Enoyl-CoA hydratase (EC 4.2.1.17), at 74,871 to 75,632
GFF1482
GFF1483 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 75,620 to 76,423
GFF1483
Position (kb) 73
74
75 Strain fitness (log2 ratio) -2
-1
0
1
2 at 72.780 kb on - strand, within GFF1480 at 72.795 kb on - strand, within GFF1480 at 72.795 kb on - strand, within GFF1480 at 72.797 kb on + strand, within GFF1480 at 72.798 kb on - strand, within GFF1480 at 72.992 kb on + strand, within GFF1480 at 73.080 kb on - strand, within GFF1480 at 73.080 kb on - strand, within GFF1480 at 73.148 kb on + strand, within GFF1480 at 73.148 kb on + strand, within GFF1480 at 73.149 kb on - strand, within GFF1480 at 73.202 kb on + strand, within GFF1480 at 73.202 kb on + strand, within GFF1480 at 73.203 kb on - strand, within GFF1480 at 73.287 kb on - strand, within GFF1480 at 73.287 kb on - strand, within GFF1480 at 73.304 kb on + strand, within GFF1480 at 73.304 kb on + strand, within GFF1480 at 73.305 kb on - strand, within GFF1480 at 73.307 kb on + strand, within GFF1480 at 73.308 kb on - strand, within GFF1480 at 73.308 kb on - strand, within GFF1480 at 73.427 kb on + strand at 73.428 kb on - strand at 73.441 kb on + strand at 73.441 kb on + strand at 73.441 kb on + strand at 73.442 kb on - strand at 73.605 kb on - strand at 73.612 kb on - strand at 73.612 kb on - strand at 73.613 kb on + strand at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 74.020 kb on + strand, within GFF1481 at 75.273 kb on + strand, within GFF1482 at 75.273 kb on + strand, within GFF1482 at 75.273 kb on + strand, within GFF1482 at 75.276 kb on + strand, within GFF1482 at 75.436 kb on - strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.512 kb on + strand, within GFF1482 at 75.543 kb on + strand, within GFF1482 at 75.740 kb on - strand, within GFF1483
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 72,780 - GFF1480 0.49 -0.0 72,795 - GFF1480 0.50 -0.2 72,795 - GFF1480 0.50 -0.2 72,797 + GFF1480 0.50 +0.1 72,798 - GFF1480 0.50 +0.2 72,992 + GFF1480 0.62 -0.5 73,080 - GFF1480 0.68 -0.7 73,080 - GFF1480 0.68 -0.6 73,148 + GFF1480 0.73 +0.2 73,148 + GFF1480 0.73 +2.0 73,149 - GFF1480 0.73 +0.4 73,202 + GFF1480 0.76 -0.4 73,202 + GFF1480 0.76 -1.0 73,203 - GFF1480 0.76 +0.1 73,287 - GFF1480 0.82 -0.1 73,287 - GFF1480 0.82 -0.3 73,304 + GFF1480 0.83 -0.5 73,304 + GFF1480 0.83 -1.3 73,305 - GFF1480 0.83 -0.5 73,307 + GFF1480 0.83 -0.1 73,308 - GFF1480 0.83 -0.1 73,308 - GFF1480 0.83 -1.2 73,427 + -1.2 73,428 - -0.5 73,441 + -0.3 73,441 + -1.0 73,441 + +0.3 73,442 - -0.2 73,605 - -1.8 73,612 - +0.8 73,612 - +0.5 73,613 + -0.2 73,834 + GFF1481 0.16 +0.0 73,834 + GFF1481 0.16 -1.8 73,834 + GFF1481 0.16 -0.2 73,834 + GFF1481 0.16 +2.3 73,834 + GFF1481 0.16 +0.8 73,837 + GFF1481 0.17 +0.2 73,837 + GFF1481 0.17 -1.5 73,837 + GFF1481 0.17 +0.1 73,837 + GFF1481 0.17 -0.5 73,837 + GFF1481 0.17 -1.6 73,984 + GFF1481 0.28 +0.2 73,984 + GFF1481 0.28 +0.1 73,984 + GFF1481 0.28 +0.2 73,985 - GFF1481 0.29 -0.4 73,985 - GFF1481 0.29 -1.2 73,985 - GFF1481 0.29 -0.7 74,020 + GFF1481 0.31 +0.3 75,273 + GFF1482 0.53 -0.2 75,273 + GFF1482 0.53 -2.2 75,273 + GFF1482 0.53 +0.8 75,276 + GFF1482 0.53 +1.6 75,436 - GFF1482 0.74 -1.2 75,472 + GFF1482 0.79 -0.3 75,472 + GFF1482 0.79 +0.2 75,472 + GFF1482 0.79 +0.5 75,512 + GFF1482 0.84 +0.0 75,543 + GFF1482 0.88 +0.1 75,740 - GFF1483 0.15 -0.2
Or see this region's nucleotide sequence