Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1475 and GFF1476 are separated by 89 nucleotides GFF1476 and GFF1477 are separated by 6 nucleotides GFF1477 and GFF1478 are separated by 45 nucleotides GFF1478 and GFF1479 are separated by 33 nucleotides
GFF1475 - no description, at 67,109 to 67,747
GFF1475
GFF1476 - hypothetical protein, at 67,837 to 68,583
GFF1476
GFF1477 - BUG/TctC family periplasmic protein, at 68,590 to 69,558
GFF1477
GFF1478 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 69,604 to 70,482
GFF1478
GFF1479 - Aldehyde dehydrogenase (EC 1.2.1.3), at 70,516 to 72,012
GFF1479
Position (kb) 68
69
70 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 67.807 kb on + strand at 67.807 kb on + strand at 67.808 kb on - strand at 67.808 kb on - strand at 67.849 kb on + strand at 67.849 kb on + strand at 67.850 kb on - strand at 67.953 kb on - strand, within GFF1476 at 67.953 kb on - strand, within GFF1476 at 68.068 kb on + strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.072 kb on + strand, within GFF1476 at 68.072 kb on + strand, within GFF1476 at 68.073 kb on - strand, within GFF1476 at 68.252 kb on + strand, within GFF1476 at 68.252 kb on + strand, within GFF1476 at 68.253 kb on - strand, within GFF1476 at 68.273 kb on + strand, within GFF1476 at 68.274 kb on - strand, within GFF1476 at 68.310 kb on - strand, within GFF1476 at 68.318 kb on + strand, within GFF1476 at 68.318 kb on + strand, within GFF1476 at 68.330 kb on + strand, within GFF1476 at 68.571 kb on + strand at 68.571 kb on + strand at 68.572 kb on - strand at 69.128 kb on + strand, within GFF1477 at 69.129 kb on - strand, within GFF1477 at 69.129 kb on - strand, within GFF1477 at 69.129 kb on - strand, within GFF1477 at 69.197 kb on + strand, within GFF1477 at 69.237 kb on - strand, within GFF1477 at 69.677 kb on + strand at 69.678 kb on - strand at 69.836 kb on + strand, within GFF1478 at 70.238 kb on + strand, within GFF1478
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 67,807 + -0.2 67,807 + +2.1 67,808 - -0.1 67,808 - +2.3 67,849 + +0.6 67,849 + -0.2 67,850 - -1.2 67,953 - GFF1476 0.16 +0.2 67,953 - GFF1476 0.16 +0.5 68,068 + GFF1476 0.31 -1.3 68,069 - GFF1476 0.31 -0.2 68,069 - GFF1476 0.31 -2.2 68,069 - GFF1476 0.31 +1.4 68,069 - GFF1476 0.31 +2.8 68,072 + GFF1476 0.31 +1.2 68,072 + GFF1476 0.31 -0.8 68,073 - GFF1476 0.32 -2.4 68,252 + GFF1476 0.56 +0.5 68,252 + GFF1476 0.56 -2.0 68,253 - GFF1476 0.56 +0.4 68,273 + GFF1476 0.58 -0.9 68,274 - GFF1476 0.59 -0.2 68,310 - GFF1476 0.63 -0.2 68,318 + GFF1476 0.64 +0.2 68,318 + GFF1476 0.64 +0.8 68,330 + GFF1476 0.66 -0.1 68,571 + -0.8 68,571 + -0.2 68,572 - -0.1 69,128 + GFF1477 0.56 +1.8 69,129 - GFF1477 0.56 -0.1 69,129 - GFF1477 0.56 +0.5 69,129 - GFF1477 0.56 +1.5 69,197 + GFF1477 0.63 +0.3 69,237 - GFF1477 0.67 -0.2 69,677 + -0.4 69,678 - -0.2 69,836 + GFF1478 0.26 +1.0 70,238 + GFF1478 0.72 -0.9
Or see this region's nucleotide sequence