Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1345 and GFF1346 overlap by 1 nucleotides GFF1346 and GFF1347 are separated by 15 nucleotides GFF1347 and GFF1348 are separated by 28 nucleotides GFF1348 and GFF1349 are separated by 9 nucleotides GFF1349 and GFF1350 are separated by 24 nucleotides
GFF1345 - 3-methylmercaptopropionyl-CoA dehydrogenase (DmdC), at 688,824 to 690,677
GFF1345
GFF1346 - hypothetical protein, at 690,677 to 690,928
GFF1346
GFF1347 - Dabb, at 690,944 to 691,246
GFF1347
GFF1348 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 691,275 to 692,072
GFF1348
GFF1349 - Thioesterase, at 692,082 to 692,546
GFF1349
GFF1350 - Quinone oxidoreductase (EC 1.6.5.5), at 692,571 to 693,587
GFF1350
Position (kb) 691
692
693 Strain fitness (log2 ratio) -2
-1
0
1
2 at 690.456 kb on + strand, within GFF1345 at 690.456 kb on + strand, within GFF1345 at 690.456 kb on + strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.579 kb on + strand at 690.580 kb on - strand at 690.591 kb on + strand at 690.591 kb on + strand at 690.782 kb on + strand, within GFF1346 at 690.782 kb on + strand, within GFF1346 at 690.782 kb on + strand, within GFF1346 at 690.783 kb on - strand, within GFF1346 at 690.783 kb on - strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.926 kb on + strand at 691.109 kb on + strand, within GFF1347 at 691.109 kb on + strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.161 kb on - strand, within GFF1347 at 691.235 kb on + strand at 691.235 kb on + strand at 691.235 kb on + strand at 691.236 kb on - strand at 691.650 kb on + strand, within GFF1348 at 691.684 kb on - strand, within GFF1348 at 691.773 kb on + strand, within GFF1348 at 691.774 kb on - strand, within GFF1348 at 691.774 kb on - strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 692.199 kb on + strand, within GFF1349 at 692.200 kb on - strand, within GFF1349 at 692.200 kb on - strand, within GFF1349 at 692.431 kb on - strand, within GFF1349 at 692.431 kb on - strand, within GFF1349 at 692.478 kb on + strand, within GFF1349 at 692.566 kb on + strand at 692.567 kb on - strand at 692.567 kb on - strand at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.716 kb on - strand, within GFF1350 at 692.716 kb on - strand, within GFF1350 at 692.748 kb on + strand, within GFF1350 at 692.748 kb on + strand, within GFF1350 at 692.749 kb on - strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.871 kb on + strand, within GFF1350 at 692.871 kb on + strand, within GFF1350 at 692.872 kb on - strand, within GFF1350 at 692.872 kb on - strand, within GFF1350 at 692.983 kb on - strand, within GFF1350
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 690,456 + GFF1345 0.88 -0.1 690,456 + GFF1345 0.88 -0.0 690,456 + GFF1345 0.88 +1.1 690,457 - GFF1345 0.88 -1.0 690,457 - GFF1345 0.88 -0.8 690,457 - GFF1345 0.88 +1.1 690,457 - GFF1345 0.88 +0.2 690,457 - GFF1345 0.88 -0.8 690,579 + -1.4 690,580 - -0.1 690,591 + +0.1 690,591 + +0.8 690,782 + GFF1346 0.42 -0.5 690,782 + GFF1346 0.42 +0.1 690,782 + GFF1346 0.42 +0.0 690,783 - GFF1346 0.42 -0.5 690,783 - GFF1346 0.42 -1.7 690,899 + GFF1346 0.88 -1.1 690,899 + GFF1346 0.88 -0.3 690,899 + GFF1346 0.88 -0.5 690,899 + GFF1346 0.88 -0.4 690,900 - GFF1346 0.88 -1.1 690,900 - GFF1346 0.88 -1.1 690,900 - GFF1346 0.88 +0.1 690,900 - GFF1346 0.88 -0.6 690,900 - GFF1346 0.88 -0.2 690,900 - GFF1346 0.88 -0.9 690,900 - GFF1346 0.88 +1.5 690,900 - GFF1346 0.88 -0.1 690,926 + -0.6 691,109 + GFF1347 0.54 +0.5 691,109 + GFF1347 0.54 -0.2 691,110 - GFF1347 0.55 +0.5 691,110 - GFF1347 0.55 -0.1 691,110 - GFF1347 0.55 -2.3 691,161 - GFF1347 0.72 -0.1 691,235 + -0.0 691,235 + -0.5 691,235 + +0.6 691,236 - -0.7 691,650 + GFF1348 0.47 -0.8 691,684 - GFF1348 0.51 +0.3 691,773 + GFF1348 0.62 -0.2 691,774 - GFF1348 0.63 -0.3 691,774 - GFF1348 0.63 -0.1 691,836 + GFF1348 0.70 -1.3 691,836 + GFF1348 0.70 +0.0 691,836 + GFF1348 0.70 -0.7 691,837 - GFF1348 0.70 -0.8 691,837 - GFF1348 0.70 -1.7 691,837 - GFF1348 0.70 -0.2 691,837 - GFF1348 0.70 -1.1 692,199 + GFF1349 0.25 +0.8 692,200 - GFF1349 0.25 -1.3 692,200 - GFF1349 0.25 -0.0 692,431 - GFF1349 0.75 -1.1 692,431 - GFF1349 0.75 +0.0 692,478 + GFF1349 0.85 +0.0 692,566 + -0.4 692,567 - -1.2 692,567 - -0.3 692,697 + GFF1350 0.12 -0.7 692,697 + GFF1350 0.12 +0.3 692,697 + GFF1350 0.12 -0.3 692,697 + GFF1350 0.12 -0.8 692,697 + GFF1350 0.12 +0.9 692,697 + GFF1350 0.12 -0.2 692,698 - GFF1350 0.12 -1.5 692,698 - GFF1350 0.12 -0.1 692,698 - GFF1350 0.12 -2.6 692,698 - GFF1350 0.12 +0.2 692,698 - GFF1350 0.12 -0.1 692,698 - GFF1350 0.12 -0.4 692,698 - GFF1350 0.12 -1.5 692,698 - GFF1350 0.12 +0.1 692,698 - GFF1350 0.12 -1.6 692,715 + GFF1350 0.14 +0.0 692,715 + GFF1350 0.14 +0.3 692,715 + GFF1350 0.14 +0.6 692,716 - GFF1350 0.14 -0.5 692,716 - GFF1350 0.14 -0.7 692,748 + GFF1350 0.17 +0.4 692,748 + GFF1350 0.17 +0.0 692,749 - GFF1350 0.18 +1.8 692,826 + GFF1350 0.25 -2.3 692,826 + GFF1350 0.25 -0.4 692,826 + GFF1350 0.25 -0.3 692,827 - GFF1350 0.25 +0.6 692,827 - GFF1350 0.25 +0.1 692,827 - GFF1350 0.25 -1.3 692,827 - GFF1350 0.25 -1.1 692,827 - GFF1350 0.25 +0.1 692,871 + GFF1350 0.29 -1.0 692,871 + GFF1350 0.29 -0.5 692,872 - GFF1350 0.30 +0.2 692,872 - GFF1350 0.30 -0.4 692,983 - GFF1350 0.41 +1.0
Or see this region's nucleotide sequence