Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1216 and GFF1217 overlap by 4 nucleotides GFF1217 and GFF1218 are separated by 207 nucleotides
GFF1216 - Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase, at 536,171 to 537,142
GFF1216
GFF1217 - Transcriptional regulator RpiR in protein degradation cluster, at 537,139 to 538,140
GFF1217
GFF1218 - O-antigen acetylase, at 538,348 to 539,502
GFF1218
Position (kb)
537
538
539 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 536.223 kb on + strand at 536.223 kb on + strand at 536.223 kb on + strand at 536.223 kb on + strand at 536.223 kb on + strand at 536.223 kb on + strand at 536.223 kb on + strand at 536.224 kb on - strand at 536.224 kb on - strand at 536.224 kb on - strand at 536.224 kb on - strand at 536.383 kb on - strand, within GFF1216 at 536.383 kb on - strand, within GFF1216 at 536.454 kb on + strand, within GFF1216 at 536.454 kb on + strand, within GFF1216 at 536.455 kb on - strand, within GFF1216 at 536.455 kb on - strand, within GFF1216 at 536.455 kb on - strand, within GFF1216 at 536.825 kb on + strand, within GFF1216 at 536.825 kb on + strand, within GFF1216 at 536.825 kb on + strand, within GFF1216 at 536.825 kb on + strand, within GFF1216 at 536.825 kb on + strand, within GFF1216 at 536.826 kb on - strand, within GFF1216 at 536.826 kb on - strand, within GFF1216 at 536.826 kb on - strand, within GFF1216 at 536.826 kb on - strand, within GFF1216 at 536.826 kb on - strand, within GFF1216 at 537.051 kb on + strand at 537.052 kb on - strand at 537.052 kb on - strand at 537.052 kb on - strand at 537.231 kb on + strand at 537.231 kb on + strand at 537.231 kb on + strand at 537.231 kb on + strand at 537.231 kb on + strand at 537.231 kb on + strand at 537.232 kb on - strand at 537.232 kb on - strand at 537.232 kb on - strand at 537.232 kb on - strand at 537.401 kb on + strand, within GFF1217 at 537.401 kb on + strand, within GFF1217 at 537.402 kb on - strand, within GFF1217 at 537.537 kb on - strand, within GFF1217 at 537.710 kb on + strand, within GFF1217 at 537.710 kb on + strand, within GFF1217 at 537.710 kb on + strand, within GFF1217 at 537.710 kb on + strand, within GFF1217 at 537.711 kb on - strand, within GFF1217 at 537.711 kb on - strand, within GFF1217 at 537.761 kb on + strand, within GFF1217 at 537.761 kb on + strand, within GFF1217 at 537.761 kb on + strand, within GFF1217 at 537.762 kb on - strand, within GFF1217 at 537.762 kb on - strand, within GFF1217 at 537.927 kb on - strand, within GFF1217 at 537.927 kb on - strand, within GFF1217 at 538.043 kb on + strand at 538.043 kb on + strand at 538.043 kb on + strand at 538.043 kb on + strand at 538.044 kb on - strand at 538.044 kb on - strand at 538.044 kb on - strand at 538.044 kb on - strand at 538.044 kb on - strand at 538.044 kb on - strand at 538.193 kb on + strand at 538.243 kb on - strand at 538.243 kb on - strand at 538.311 kb on + strand at 538.311 kb on + strand at 538.312 kb on - strand at 538.312 kb on - strand at 538.312 kb on - strand at 538.312 kb on - strand at 538.312 kb on - strand at 538.334 kb on + strand at 538.334 kb on + strand at 538.453 kb on + strand at 538.453 kb on + strand at 538.528 kb on + strand, within GFF1218 at 538.528 kb on + strand, within GFF1218 at 538.528 kb on + strand, within GFF1218 at 538.529 kb on - strand, within GFF1218 at 538.561 kb on + strand, within GFF1218 at 538.562 kb on - strand, within GFF1218 at 538.562 kb on - strand, within GFF1218 at 538.591 kb on + strand, within GFF1218 at 538.627 kb on + strand, within GFF1218 at 538.627 kb on + strand, within GFF1218 at 538.627 kb on + strand, within GFF1218 at 538.627 kb on + strand, within GFF1218 at 538.627 kb on + strand, within GFF1218 at 538.628 kb on - strand, within GFF1218 at 538.628 kb on - strand, within GFF1218 at 538.628 kb on - strand, within GFF1218 at 538.628 kb on - strand, within GFF1218 at 538.825 kb on + strand, within GFF1218 at 538.825 kb on + strand, within GFF1218 at 538.825 kb on + strand, within GFF1218 at 538.840 kb on + strand, within GFF1218 at 538.840 kb on + strand, within GFF1218 at 538.840 kb on + strand, within GFF1218 at 538.840 kb on + strand, within GFF1218 at 538.840 kb on + strand, within GFF1218 at 538.840 kb on + strand, within GFF1218 at 538.840 kb on + strand, within GFF1218 at 538.840 kb on + strand, within GFF1218 at 538.841 kb on - strand, within GFF1218 at 538.841 kb on - strand, within GFF1218 at 538.841 kb on - strand, within GFF1218 at 538.841 kb on - strand, within GFF1218 at 538.841 kb on - strand, within GFF1218 at 538.841 kb on - strand, within GFF1218 at 538.870 kb on + strand, within GFF1218 at 538.870 kb on + strand, within GFF1218 at 538.870 kb on + strand, within GFF1218 at 538.870 kb on + strand, within GFF1218 at 538.871 kb on - strand, within GFF1218 at 538.871 kb on - strand, within GFF1218 at 538.928 kb on + strand, within GFF1218 at 538.929 kb on - strand, within GFF1218 at 538.929 kb on - strand, within GFF1218 at 538.990 kb on + strand, within GFF1218 at 538.990 kb on + strand, within GFF1218 at 538.990 kb on + strand, within GFF1218 at 538.990 kb on + strand, within GFF1218 at 538.990 kb on + strand, within GFF1218 at 538.990 kb on + strand, within GFF1218 at 538.990 kb on + strand, within GFF1218 at 538.990 kb on + strand, within GFF1218 at 538.991 kb on - strand, within GFF1218 at 538.991 kb on - strand, within GFF1218 at 538.991 kb on - strand, within GFF1218 at 538.991 kb on - strand, within GFF1218 at 538.991 kb on - strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 538.999 kb on + strand, within GFF1218 at 539.000 kb on - strand, within GFF1218 at 539.000 kb on - strand, within GFF1218 at 539.000 kb on - strand, within GFF1218 at 539.000 kb on - strand, within GFF1218 at 539.000 kb on - strand, within GFF1218 at 539.000 kb on - strand, within GFF1218 at 539.000 kb on - strand, within GFF1218 at 539.001 kb on - strand, within GFF1218 at 539.131 kb on + strand, within GFF1218 at 539.131 kb on + strand, within GFF1218 at 539.131 kb on + strand, within GFF1218 at 539.132 kb on - strand, within GFF1218 at 539.132 kb on - strand, within GFF1218 at 539.132 kb on - strand, within GFF1218
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 536,223 + -0.7 536,223 + -0.4 536,223 + +0.3 536,223 + -1.0 536,223 + -0.1 536,223 + -0.7 536,223 + -0.8 536,224 - -0.4 536,224 - +0.2 536,224 - -1.0 536,224 - -0.4 536,383 - GFF1216 0.22 -0.2 536,383 - GFF1216 0.22 +0.2 536,454 + GFF1216 0.29 +0.6 536,454 + GFF1216 0.29 -1.1 536,455 - GFF1216 0.29 -0.8 536,455 - GFF1216 0.29 +0.8 536,455 - GFF1216 0.29 -1.2 536,825 + GFF1216 0.67 -0.2 536,825 + GFF1216 0.67 +0.7 536,825 + GFF1216 0.67 +0.5 536,825 + GFF1216 0.67 +0.8 536,825 + GFF1216 0.67 -0.5 536,826 - GFF1216 0.67 -0.1 536,826 - GFF1216 0.67 +0.3 536,826 - GFF1216 0.67 +0.5 536,826 - GFF1216 0.67 -0.1 536,826 - GFF1216 0.67 -0.2 537,051 + -0.4 537,052 - -1.1 537,052 - -0.2 537,052 - -1.0 537,231 + -0.9 537,231 + -0.1 537,231 + +0.2 537,231 + -0.3 537,231 + +0.5 537,231 + +0.1 537,232 - -2.2 537,232 - -0.6 537,232 - -0.4 537,232 - -0.5 537,401 + GFF1217 0.26 -0.1 537,401 + GFF1217 0.26 -0.4 537,402 - GFF1217 0.26 +0.2 537,537 - GFF1217 0.40 -1.1 537,710 + GFF1217 0.57 -0.4 537,710 + GFF1217 0.57 +1.0 537,710 + GFF1217 0.57 +0.8 537,710 + GFF1217 0.57 -0.3 537,711 - GFF1217 0.57 -0.3 537,711 - GFF1217 0.57 -0.1 537,761 + GFF1217 0.62 -1.3 537,761 + GFF1217 0.62 -0.7 537,761 + GFF1217 0.62 +0.6 537,762 - GFF1217 0.62 -0.4 537,762 - GFF1217 0.62 -1.9 537,927 - GFF1217 0.79 -0.2 537,927 - GFF1217 0.79 -0.7 538,043 + -1.5 538,043 + -1.1 538,043 + -0.3 538,043 + +0.2 538,044 - +0.8 538,044 - -0.2 538,044 - +1.5 538,044 - -1.1 538,044 - -1.3 538,044 - -0.4 538,193 + +0.3 538,243 - +0.1 538,243 - -0.1 538,311 + -1.9 538,311 + +0.3 538,312 - -0.4 538,312 - -0.9 538,312 - -0.8 538,312 - -1.8 538,312 - +1.5 538,334 + -0.1 538,334 + +0.5 538,453 + -0.1 538,453 + -0.2 538,528 + GFF1218 0.16 -0.6 538,528 + GFF1218 0.16 -0.8 538,528 + GFF1218 0.16 -0.4 538,529 - GFF1218 0.16 -1.7 538,561 + GFF1218 0.18 -0.6 538,562 - GFF1218 0.19 +1.0 538,562 - GFF1218 0.19 -0.2 538,591 + GFF1218 0.21 -0.1 538,627 + GFF1218 0.24 -0.3 538,627 + GFF1218 0.24 -0.9 538,627 + GFF1218 0.24 -0.3 538,627 + GFF1218 0.24 -0.4 538,627 + GFF1218 0.24 -0.3 538,628 - GFF1218 0.24 +0.2 538,628 - GFF1218 0.24 +0.3 538,628 - GFF1218 0.24 +0.1 538,628 - GFF1218 0.24 -0.4 538,825 + GFF1218 0.41 +0.4 538,825 + GFF1218 0.41 +0.1 538,825 + GFF1218 0.41 +0.9 538,840 + GFF1218 0.43 -1.1 538,840 + GFF1218 0.43 -1.7 538,840 + GFF1218 0.43 -0.2 538,840 + GFF1218 0.43 +0.6 538,840 + GFF1218 0.43 +0.6 538,840 + GFF1218 0.43 -0.2 538,840 + GFF1218 0.43 -0.5 538,840 + GFF1218 0.43 -1.8 538,841 - GFF1218 0.43 +0.2 538,841 - GFF1218 0.43 +0.1 538,841 - GFF1218 0.43 -0.1 538,841 - GFF1218 0.43 -1.1 538,841 - GFF1218 0.43 -1.2 538,841 - GFF1218 0.43 -1.1 538,870 + GFF1218 0.45 +0.7 538,870 + GFF1218 0.45 +0.6 538,870 + GFF1218 0.45 +1.2 538,870 + GFF1218 0.45 -1.4 538,871 - GFF1218 0.45 -0.7 538,871 - GFF1218 0.45 +2.0 538,928 + GFF1218 0.50 +2.1 538,929 - GFF1218 0.50 +0.3 538,929 - GFF1218 0.50 -0.4 538,990 + GFF1218 0.56 -0.1 538,990 + GFF1218 0.56 -1.3 538,990 + GFF1218 0.56 -0.0 538,990 + GFF1218 0.56 +0.1 538,990 + GFF1218 0.56 -0.4 538,990 + GFF1218 0.56 -0.9 538,990 + GFF1218 0.56 +0.7 538,990 + GFF1218 0.56 -0.5 538,991 - GFF1218 0.56 +0.4 538,991 - GFF1218 0.56 -0.4 538,991 - GFF1218 0.56 +0.6 538,991 - GFF1218 0.56 -0.2 538,991 - GFF1218 0.56 -1.1 538,999 + GFF1218 0.56 +0.7 538,999 + GFF1218 0.56 -2.6 538,999 + GFF1218 0.56 -1.2 538,999 + GFF1218 0.56 +0.7 538,999 + GFF1218 0.56 +0.8 538,999 + GFF1218 0.56 +0.8 538,999 + GFF1218 0.56 -1.9 538,999 + GFF1218 0.56 -0.4 538,999 + GFF1218 0.56 -1.7 538,999 + GFF1218 0.56 -0.5 538,999 + GFF1218 0.56 -0.1 538,999 + GFF1218 0.56 -0.1 538,999 + GFF1218 0.56 -0.2 538,999 + GFF1218 0.56 -0.6 538,999 + GFF1218 0.56 -1.4 539,000 - GFF1218 0.56 -1.1 539,000 - GFF1218 0.56 +0.8 539,000 - GFF1218 0.56 +1.5 539,000 - GFF1218 0.56 +0.4 539,000 - GFF1218 0.56 +0.3 539,000 - GFF1218 0.56 -0.1 539,000 - GFF1218 0.56 +0.2 539,001 - GFF1218 0.57 +0.2 539,131 + GFF1218 0.68 -0.2 539,131 + GFF1218 0.68 -0.7 539,131 + GFF1218 0.68 -0.2 539,132 - GFF1218 0.68 +0.2 539,132 - GFF1218 0.68 +0.5 539,132 - GFF1218 0.68 -0.4
Or see this region's nucleotide sequence