Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF941 and GFF942 overlap by 4 nucleotides GFF942 and GFF943 are separated by 19 nucleotides GFF943 and GFF944 are separated by 158 nucleotides
GFF941 - Transporter, LysE family, at 254,505 to 255,143
GFF941
GFF942 - Iron-sulfur cluster-binding protein, at 255,140 to 255,775
GFF942
GFF943 - Intracellular PHB depolymerase, at 255,795 to 257,237
GFF943
GFF944 - Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 257,396 to 258,592
GFF944
Position (kb) 255
256
257
258 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 254.874 kb on + strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.899 kb on + strand, within GFF941 at 254.899 kb on + strand, within GFF941 at 254.899 kb on + strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.919 kb on + strand, within GFF941 at 254.920 kb on - strand, within GFF941 at 254.920 kb on - strand, within GFF941 at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.309 kb on + strand, within GFF942 at 255.310 kb on - strand, within GFF942 at 255.310 kb on - strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.694 kb on - strand, within GFF942 at 255.694 kb on - strand, within GFF942 at 255.694 kb on - strand, within GFF942 at 256.015 kb on + strand, within GFF943 at 256.054 kb on + strand, within GFF943 at 256.054 kb on + strand, within GFF943 at 256.078 kb on + strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.278 kb on + strand, within GFF943 at 256.278 kb on + strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.301 kb on - strand, within GFF943 at 256.301 kb on - strand, within GFF943 at 256.301 kb on - strand, within GFF943 at 256.301 kb on - strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.304 kb on - strand, within GFF943 at 256.304 kb on - strand, within GFF943 at 256.304 kb on - strand, within GFF943 at 256.304 kb on - strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.334 kb on - strand, within GFF943 at 256.342 kb on + strand, within GFF943 at 256.342 kb on + strand, within GFF943 at 256.342 kb on + strand, within GFF943 at 256.343 kb on - strand, within GFF943 at 256.343 kb on - strand, within GFF943 at 256.343 kb on - strand, within GFF943 at 256.399 kb on + strand, within GFF943 at 256.399 kb on + strand, within GFF943 at 256.400 kb on - strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.472 kb on - strand, within GFF943 at 256.513 kb on + strand, within GFF943 at 256.513 kb on + strand, within GFF943 at 256.514 kb on - strand, within GFF943 at 256.514 kb on - strand, within GFF943 at 256.514 kb on - strand, within GFF943 at 256.714 kb on + strand, within GFF943 at 256.715 kb on - strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.825 kb on + strand, within GFF943 at 256.825 kb on + strand, within GFF943 at 256.825 kb on + strand, within GFF943 at 256.825 kb on + strand, within GFF943 at 256.825 kb on + strand, within GFF943 at 256.825 kb on + strand, within GFF943 at 256.826 kb on - strand, within GFF943 at 256.826 kb on - strand, within GFF943 at 256.826 kb on - strand, within GFF943 at 256.826 kb on - strand, within GFF943 at 256.826 kb on - strand, within GFF943 at 256.826 kb on - strand, within GFF943 at 256.826 kb on - strand, within GFF943 at 256.880 kb on - strand, within GFF943 at 256.880 kb on - strand, within GFF943 at 257.071 kb on + strand, within GFF943 at 257.071 kb on + strand, within GFF943 at 257.071 kb on + strand, within GFF943 at 257.071 kb on + strand, within GFF943 at 257.072 kb on - strand, within GFF943 at 257.072 kb on - strand, within GFF943 at 257.072 kb on - strand, within GFF943 at 257.072 kb on - strand, within GFF943 at 257.072 kb on - strand, within GFF943 at 257.072 kb on - strand, within GFF943 at 257.089 kb on + strand, within GFF943 at 257.089 kb on + strand, within GFF943 at 257.089 kb on + strand, within GFF943 at 257.090 kb on - strand, within GFF943 at 257.090 kb on - strand, within GFF943 at 257.101 kb on + strand at 257.101 kb on + strand at 257.101 kb on + strand at 257.101 kb on + strand at 257.101 kb on + strand at 257.101 kb on + strand at 257.101 kb on + strand at 257.102 kb on - strand at 257.102 kb on - strand at 257.102 kb on - strand at 257.158 kb on + strand at 257.158 kb on + strand at 257.158 kb on + strand at 257.159 kb on - strand at 257.159 kb on - strand at 257.159 kb on - strand at 257.159 kb on - strand at 257.221 kb on + strand at 257.221 kb on + strand at 257.221 kb on + strand at 257.221 kb on + strand at 257.222 kb on - strand at 257.222 kb on - strand at 257.222 kb on - strand at 257.222 kb on - strand at 257.222 kb on - strand at 257.222 kb on - strand at 257.230 kb on + strand at 257.230 kb on + strand at 257.230 kb on + strand at 257.231 kb on - strand at 257.231 kb on - strand at 257.231 kb on - strand at 257.231 kb on - strand at 257.231 kb on - strand at 257.231 kb on - strand at 257.270 kb on + strand at 257.270 kb on + strand at 257.270 kb on + strand at 257.270 kb on + strand at 257.270 kb on + strand at 257.270 kb on + strand at 257.270 kb on + strand at 257.270 kb on + strand at 257.270 kb on + strand at 257.271 kb on - strand at 257.271 kb on - strand at 257.271 kb on - strand at 257.271 kb on - strand at 257.272 kb on + strand at 257.272 kb on + strand at 257.272 kb on + strand at 257.273 kb on - strand at 257.273 kb on - strand at 257.273 kb on - strand at 257.304 kb on - strand at 257.304 kb on - strand at 257.304 kb on - strand at 257.304 kb on - strand at 257.310 kb on + strand at 257.311 kb on - strand at 257.311 kb on - strand at 257.342 kb on + strand at 257.342 kb on + strand at 257.342 kb on + strand at 257.342 kb on + strand at 257.343 kb on - strand at 257.343 kb on - strand at 257.343 kb on - strand at 257.397 kb on + strand at 257.397 kb on + strand at 257.397 kb on + strand at 257.397 kb on + strand at 257.397 kb on + strand at 257.397 kb on + strand at 257.397 kb on + strand at 257.398 kb on - strand at 257.398 kb on - strand at 257.398 kb on - strand at 257.398 kb on - strand at 257.398 kb on - strand at 257.398 kb on - strand at 257.398 kb on - strand at 257.484 kb on + strand at 257.538 kb on + strand, within GFF944 at 257.539 kb on - strand, within GFF944 at 257.740 kb on - strand, within GFF944 at 258.139 kb on - strand, within GFF944 at 258.139 kb on - strand, within GFF944 at 258.142 kb on - strand, within GFF944
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 254,874 + GFF941 0.58 -2.3 254,874 + GFF941 0.58 -0.0 254,874 + GFF941 0.58 +0.9 254,874 + GFF941 0.58 -1.7 254,874 + GFF941 0.58 -0.2 254,875 - GFF941 0.58 -0.6 254,875 - GFF941 0.58 -0.3 254,875 - GFF941 0.58 +0.2 254,875 - GFF941 0.58 -0.6 254,875 - GFF941 0.58 -0.0 254,899 + GFF941 0.62 +1.5 254,899 + GFF941 0.62 -1.3 254,899 + GFF941 0.62 -2.2 254,900 - GFF941 0.62 -0.2 254,900 - GFF941 0.62 +1.1 254,900 - GFF941 0.62 +0.1 254,900 - GFF941 0.62 +2.5 254,900 - GFF941 0.62 +0.2 254,900 - GFF941 0.62 -1.0 254,900 - GFF941 0.62 +0.5 254,919 + GFF941 0.65 +0.2 254,920 - GFF941 0.65 -0.9 254,920 - GFF941 0.65 +0.5 255,118 + +0.5 255,118 + -0.1 255,118 + +0.7 255,118 + +0.6 255,118 + +0.4 255,118 + -0.3 255,118 + -0.7 255,118 + -0.3 255,118 + +0.7 255,118 + -2.7 255,118 + -2.1 255,118 + -0.5 255,118 + -1.5 255,118 + +0.4 255,118 + -0.2 255,118 + +0.3 255,118 + +1.5 255,118 + -0.4 255,118 + -0.6 255,118 + +0.1 255,118 + +0.7 255,118 + +0.1 255,118 + +0.6 255,118 + -0.0 255,118 + +0.1 255,119 - -1.3 255,119 - +0.3 255,119 - +0.1 255,119 - -0.1 255,119 - +0.3 255,119 - -0.4 255,119 - -1.3 255,119 - -0.3 255,119 - -1.0 255,119 - +0.9 255,119 - +0.2 255,119 - -1.7 255,119 - +0.4 255,119 - -1.4 255,119 - -0.5 255,119 - -0.4 255,119 - +0.6 255,119 - -2.0 255,119 - -0.3 255,119 - -1.6 255,119 - +0.1 255,119 - -0.3 255,119 - -0.2 255,119 - -0.1 255,309 + GFF942 0.27 -0.4 255,310 - GFF942 0.27 +2.5 255,310 - GFF942 0.27 -0.6 255,693 + GFF942 0.87 -2.3 255,693 + GFF942 0.87 -0.3 255,693 + GFF942 0.87 +0.2 255,693 + GFF942 0.87 -0.1 255,693 + GFF942 0.87 -0.9 255,693 + GFF942 0.87 -0.6 255,693 + GFF942 0.87 -0.8 255,694 - GFF942 0.87 +0.3 255,694 - GFF942 0.87 -0.5 255,694 - GFF942 0.87 +1.5 256,015 + GFF943 0.15 -0.4 256,054 + GFF943 0.18 -1.0 256,054 + GFF943 0.18 -1.2 256,078 + GFF943 0.20 +0.1 256,079 - GFF943 0.20 -1.0 256,079 - GFF943 0.20 -1.7 256,079 - GFF943 0.20 +0.6 256,079 - GFF943 0.20 -0.7 256,079 - GFF943 0.20 -0.6 256,278 + GFF943 0.33 +1.2 256,278 + GFF943 0.33 -0.3 256,279 - GFF943 0.34 -1.3 256,279 - GFF943 0.34 -1.2 256,279 - GFF943 0.34 -1.0 256,279 - GFF943 0.34 -1.0 256,279 - GFF943 0.34 -1.5 256,300 + GFF943 0.35 -1.0 256,300 + GFF943 0.35 -1.1 256,300 + GFF943 0.35 +0.1 256,300 + GFF943 0.35 -2.6 256,300 + GFF943 0.35 +0.6 256,301 - GFF943 0.35 -0.3 256,301 - GFF943 0.35 -0.5 256,301 - GFF943 0.35 -0.3 256,301 - GFF943 0.35 -0.3 256,303 + GFF943 0.35 -1.5 256,303 + GFF943 0.35 -1.3 256,303 + GFF943 0.35 -0.5 256,303 + GFF943 0.35 -1.7 256,303 + GFF943 0.35 -0.5 256,303 + GFF943 0.35 -0.7 256,304 - GFF943 0.35 -0.5 256,304 - GFF943 0.35 -0.6 256,304 - GFF943 0.35 -0.5 256,304 - GFF943 0.35 -0.7 256,333 + GFF943 0.37 +0.2 256,333 + GFF943 0.37 +0.7 256,333 + GFF943 0.37 +0.1 256,333 + GFF943 0.37 +0.0 256,333 + GFF943 0.37 -0.8 256,333 + GFF943 0.37 -0.4 256,333 + GFF943 0.37 -0.5 256,333 + GFF943 0.37 -2.2 256,334 - GFF943 0.37 -0.1 256,342 + GFF943 0.38 +0.0 256,342 + GFF943 0.38 -2.0 256,342 + GFF943 0.38 -0.4 256,343 - GFF943 0.38 -0.5 256,343 - GFF943 0.38 +0.1 256,343 - GFF943 0.38 -1.3 256,399 + GFF943 0.42 -1.1 256,399 + GFF943 0.42 -1.1 256,400 - GFF943 0.42 -3.1 256,405 + GFF943 0.42 -2.5 256,405 + GFF943 0.42 +1.0 256,405 + GFF943 0.42 +0.1 256,405 + GFF943 0.42 -0.6 256,405 + GFF943 0.42 +2.5 256,405 + GFF943 0.42 -0.2 256,405 + GFF943 0.42 -1.6 256,406 - GFF943 0.42 -1.1 256,406 - GFF943 0.42 -0.8 256,406 - GFF943 0.42 -0.5 256,406 - GFF943 0.42 -0.3 256,406 - GFF943 0.42 -0.1 256,406 - GFF943 0.42 -1.5 256,472 - GFF943 0.47 -1.3 256,513 + GFF943 0.50 +0.1 256,513 + GFF943 0.50 -2.4 256,514 - GFF943 0.50 -0.4 256,514 - GFF943 0.50 -0.5 256,514 - GFF943 0.50 -0.6 256,714 + GFF943 0.64 -0.3 256,715 - GFF943 0.64 -0.6 256,750 + GFF943 0.66 -0.5 256,750 + GFF943 0.66 -0.8 256,750 + GFF943 0.66 -0.6 256,750 + GFF943 0.66 -1.3 256,750 + GFF943 0.66 -1.7 256,750 + GFF943 0.66 -1.6 256,750 + GFF943 0.66 -0.2 256,751 - GFF943 0.66 -1.3 256,751 - GFF943 0.66 +0.3 256,751 - GFF943 0.66 -1.0 256,751 - GFF943 0.66 -1.1 256,751 - GFF943 0.66 -2.0 256,751 - GFF943 0.66 -0.6 256,751 - GFF943 0.66 -0.0 256,759 + GFF943 0.67 -1.5 256,759 + GFF943 0.67 +0.3 256,759 + GFF943 0.67 +0.1 256,759 + GFF943 0.67 -1.1 256,759 + GFF943 0.67 -0.5 256,760 - GFF943 0.67 +0.9 256,760 - GFF943 0.67 -0.8 256,760 - GFF943 0.67 +0.5 256,760 - GFF943 0.67 +0.5 256,760 - GFF943 0.67 +0.1 256,760 - GFF943 0.67 +0.5 256,760 - GFF943 0.67 -0.1 256,760 - GFF943 0.67 -0.8 256,825 + GFF943 0.71 +0.2 256,825 + GFF943 0.71 +1.1 256,825 + GFF943 0.71 -0.5 256,825 + GFF943 0.71 -0.6 256,825 + GFF943 0.71 +0.1 256,825 + GFF943 0.71 -1.1 256,826 - GFF943 0.71 -1.8 256,826 - GFF943 0.71 -1.1 256,826 - GFF943 0.71 -1.7 256,826 - GFF943 0.71 -0.6 256,826 - GFF943 0.71 -0.1 256,826 - GFF943 0.71 -0.2 256,826 - GFF943 0.71 -0.5 256,880 - GFF943 0.75 -0.7 256,880 - GFF943 0.75 -1.4 257,071 + GFF943 0.88 -0.1 257,071 + GFF943 0.88 -1.1 257,071 + GFF943 0.88 -0.3 257,071 + GFF943 0.88 -0.6 257,072 - GFF943 0.88 +0.4 257,072 - GFF943 0.88 -0.2 257,072 - GFF943 0.88 +0.5 257,072 - GFF943 0.88 -1.0 257,072 - GFF943 0.88 +0.3 257,072 - GFF943 0.88 -1.1 257,089 + GFF943 0.90 -0.5 257,089 + GFF943 0.90 -2.2 257,089 + GFF943 0.90 -0.7 257,090 - GFF943 0.90 -1.1 257,090 - GFF943 0.90 +0.2 257,101 + +1.5 257,101 + -0.6 257,101 + -0.6 257,101 + -1.3 257,101 + -1.1 257,101 + -0.0 257,101 + -0.2 257,102 - -0.8 257,102 - -0.1 257,102 - -2.0 257,158 + -1.2 257,158 + -0.6 257,158 + -0.3 257,159 - +0.0 257,159 - -1.0 257,159 - -1.0 257,159 - -1.7 257,221 + -0.3 257,221 + -0.2 257,221 + -2.6 257,221 + -0.7 257,222 - -0.6 257,222 - +0.7 257,222 - -0.1 257,222 - +0.9 257,222 - -0.4 257,222 - -0.4 257,230 + +1.5 257,230 + -1.2 257,230 + +0.3 257,231 - +0.3 257,231 - -1.4 257,231 - -1.3 257,231 - -0.6 257,231 - -0.3 257,231 - +0.7 257,270 + -0.0 257,270 + -0.3 257,270 + -0.3 257,270 + +0.3 257,270 + -0.1 257,270 + -0.7 257,270 + +0.7 257,270 + +0.2 257,270 + +0.1 257,271 - +0.5 257,271 - -0.9 257,271 - -0.7 257,271 - -0.1 257,272 + -0.3 257,272 + -0.7 257,272 + +0.4 257,273 - -0.8 257,273 - -0.0 257,273 - -1.3 257,304 - +0.0 257,304 - +0.1 257,304 - -0.7 257,304 - -0.4 257,310 + +1.5 257,311 - -0.0 257,311 - +0.5 257,342 + -1.0 257,342 + +0.0 257,342 + +0.4 257,342 + -0.8 257,343 - +0.5 257,343 - +0.3 257,343 - +0.3 257,397 + +0.7 257,397 + -0.2 257,397 + -0.1 257,397 + +0.7 257,397 + +0.6 257,397 + -0.1 257,397 + -1.1 257,398 - -1.2 257,398 - -1.4 257,398 - -0.4 257,398 - -0.1 257,398 - -0.8 257,398 - +1.5 257,398 - -0.1 257,484 + -0.8 257,538 + GFF944 0.12 -1.8 257,539 - GFF944 0.12 -2.0 257,740 - GFF944 0.29 -2.5 258,139 - GFF944 0.62 -1.5 258,139 - GFF944 0.62 -1.8 258,142 - GFF944 0.62 -1.1
Or see this region's nucleotide sequence