Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF896 and GFF897 are separated by 34 nucleotides GFF897 and GFF898 are separated by 7 nucleotides
GFF896 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 206,491 to 207,282
GFF896
GFF897 - putative oxygenase subunit, at 207,317 to 208,558
GFF897
GFF898 - putative oxygenase subunit, at 208,566 to 210,299
GFF898
Position (kb) 207
208
209 Strain fitness (log2 ratio) -2
-1
0
1
2 at 206.324 kb on - strand at 206.431 kb on - strand at 206.431 kb on - strand at 206.431 kb on - strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.435 kb on + strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.436 kb on - strand at 206.546 kb on + strand at 206.546 kb on + strand at 206.546 kb on + strand at 206.547 kb on - strand at 206.547 kb on - strand at 206.547 kb on - strand at 206.547 kb on - strand at 206.547 kb on - strand at 206.764 kb on + strand, within GFF896 at 206.764 kb on + strand, within GFF896 at 206.764 kb on + strand, within GFF896 at 206.764 kb on + strand, within GFF896 at 206.765 kb on - strand, within GFF896 at 206.765 kb on - strand, within GFF896 at 206.765 kb on - strand, within GFF896 at 206.765 kb on - strand, within GFF896 at 206.765 kb on - strand, within GFF896 at 206.765 kb on - strand, within GFF896 at 206.765 kb on - strand, within GFF896 at 206.944 kb on + strand, within GFF896 at 207.208 kb on + strand at 207.280 kb on + strand at 207.280 kb on + strand at 207.281 kb on - strand at 207.281 kb on - strand at 207.429 kb on + strand at 207.429 kb on + strand at 207.429 kb on + strand at 207.429 kb on + strand at 207.429 kb on + strand at 207.429 kb on + strand at 207.429 kb on + strand at 207.430 kb on - strand at 207.430 kb on - strand at 207.430 kb on - strand at 207.430 kb on - strand at 207.842 kb on + strand, within GFF897 at 207.842 kb on + strand, within GFF897 at 207.842 kb on + strand, within GFF897 at 207.843 kb on - strand, within GFF897 at 207.914 kb on + strand, within GFF897 at 207.915 kb on - strand, within GFF897 at 208.265 kb on + strand, within GFF897 at 208.349 kb on + strand, within GFF897 at 208.526 kb on + strand at 208.527 kb on - strand at 208.527 kb on - strand at 208.656 kb on + strand at 209.088 kb on + strand, within GFF898 at 209.088 kb on + strand, within GFF898 at 209.089 kb on - strand, within GFF898 at 209.089 kb on - strand, within GFF898 at 209.089 kb on - strand, within GFF898 at 209.161 kb on - strand, within GFF898 at 209.511 kb on + strand, within GFF898 at 209.511 kb on + strand, within GFF898 at 209.511 kb on + strand, within GFF898 at 209.511 kb on + strand, within GFF898
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 206,324 - +1.1 206,431 - +1.5 206,431 - -0.8 206,431 - +0.5 206,435 + -0.4 206,435 + -0.3 206,435 + -0.1 206,435 + -0.1 206,435 + -0.8 206,435 + -2.0 206,435 + -2.1 206,435 + -0.1 206,435 + +0.1 206,435 + +1.0 206,435 + +0.2 206,435 + +0.6 206,436 - -0.8 206,436 - -0.3 206,436 - -0.4 206,436 - -0.6 206,436 - -1.8 206,436 - -0.1 206,436 - -0.3 206,436 - -0.3 206,436 - -0.0 206,436 - +0.7 206,436 - -0.5 206,546 + +0.9 206,546 + +1.2 206,546 + +1.2 206,547 - +0.4 206,547 - +0.6 206,547 - +0.1 206,547 - +0.0 206,547 - -0.1 206,764 + GFF896 0.34 +0.3 206,764 + GFF896 0.34 -0.3 206,764 + GFF896 0.34 -0.8 206,764 + GFF896 0.34 -0.7 206,765 - GFF896 0.35 -0.1 206,765 - GFF896 0.35 -0.1 206,765 - GFF896 0.35 -1.3 206,765 - GFF896 0.35 -0.1 206,765 - GFF896 0.35 -0.8 206,765 - GFF896 0.35 +0.1 206,765 - GFF896 0.35 -1.5 206,944 + GFF896 0.57 +0.2 207,208 + +1.7 207,280 + +0.5 207,280 + -1.0 207,281 - -0.0 207,281 - -1.6 207,429 + -0.4 207,429 + -1.5 207,429 + -1.8 207,429 + -2.1 207,429 + -0.6 207,429 + +0.4 207,429 + -0.4 207,430 - +1.0 207,430 - -1.2 207,430 - +1.5 207,430 - +0.7 207,842 + GFF897 0.42 +0.3 207,842 + GFF897 0.42 -2.0 207,842 + GFF897 0.42 -0.5 207,843 - GFF897 0.42 +1.3 207,914 + GFF897 0.48 +0.1 207,915 - GFF897 0.48 +1.0 208,265 + GFF897 0.76 +0.5 208,349 + GFF897 0.83 -0.5 208,526 + +0.7 208,527 - +0.8 208,527 - +0.3 208,656 + +0.0 209,088 + GFF898 0.30 +1.8 209,088 + GFF898 0.30 -0.5 209,089 - GFF898 0.30 -0.2 209,089 - GFF898 0.30 -1.8 209,089 - GFF898 0.30 -1.1 209,161 - GFF898 0.34 -0.1 209,511 + GFF898 0.54 +0.3 209,511 + GFF898 0.54 -2.0 209,511 + GFF898 0.54 -0.8 209,511 + GFF898 0.54 -0.2
Or see this region's nucleotide sequence