Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF876 and GFF877 overlap by 4 nucleotides GFF877 and GFF878 are separated by 112 nucleotides GFF878 and GFF879 are separated by 228 nucleotides
GFF876 - hypothetical protein, at 184,848 to 186,509
GFF876
GFF877 - putative membrane protein, at 186,506 to 187,498
GFF877
GFF878 - COGs COG2343, at 187,611 to 188,000
GFF878
GFF879 - BUG/TctC family periplasmic protein, at 188,229 to 189,305
GFF879
Position (kb) 186
187
188 Strain fitness (log2 ratio) -1
0
1 at 185.680 kb on + strand, within GFF876 at 185.681 kb on - strand, within GFF876 at 186.307 kb on + strand, within GFF876 at 186.307 kb on + strand, within GFF876 at 186.307 kb on + strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.308 kb on - strand, within GFF876 at 186.787 kb on - strand, within GFF877 at 187.011 kb on + strand, within GFF877 at 187.012 kb on - strand, within GFF877 at 187.150 kb on - strand, within GFF877 at 187.161 kb on + strand, within GFF877 at 187.162 kb on - strand, within GFF877 at 187.162 kb on - strand, within GFF877 at 187.388 kb on + strand, within GFF877 at 187.476 kb on + strand at 187.476 kb on + strand at 187.651 kb on + strand, within GFF878 at 187.691 kb on - strand, within GFF878 at 187.702 kb on + strand, within GFF878 at 187.702 kb on + strand, within GFF878 at 187.703 kb on - strand, within GFF878 at 187.750 kb on + strand, within GFF878 at 187.750 kb on + strand, within GFF878 at 187.751 kb on - strand, within GFF878 at 187.751 kb on - strand, within GFF878 at 187.753 kb on + strand, within GFF878 at 187.754 kb on - strand, within GFF878 at 187.754 kb on - strand, within GFF878 at 187.771 kb on + strand, within GFF878 at 187.772 kb on - strand, within GFF878 at 187.772 kb on - strand, within GFF878 at 187.780 kb on + strand, within GFF878 at 187.780 kb on + strand, within GFF878 at 187.781 kb on - strand, within GFF878 at 187.837 kb on + strand, within GFF878 at 187.837 kb on + strand, within GFF878 at 187.837 kb on + strand, within GFF878 at 187.837 kb on + strand, within GFF878 at 187.837 kb on + strand, within GFF878 at 187.838 kb on - strand, within GFF878 at 187.838 kb on - strand, within GFF878 at 187.838 kb on - strand, within GFF878 at 187.838 kb on - strand, within GFF878 at 188.212 kb on + strand at 188.212 kb on + strand at 188.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 185,680 + GFF876 0.50 -0.4 185,681 - GFF876 0.50 -0.5 186,307 + GFF876 0.88 +0.4 186,307 + GFF876 0.88 -0.1 186,307 + GFF876 0.88 -0.6 186,308 - GFF876 0.88 +0.1 186,308 - GFF876 0.88 -0.6 186,308 - GFF876 0.88 -0.3 186,308 - GFF876 0.88 +0.2 186,308 - GFF876 0.88 -1.7 186,308 - GFF876 0.88 -0.7 186,787 - GFF877 0.28 -0.9 187,011 + GFF877 0.51 -0.3 187,012 - GFF877 0.51 -1.4 187,150 - GFF877 0.65 -0.2 187,161 + GFF877 0.66 +0.9 187,162 - GFF877 0.66 -0.5 187,162 - GFF877 0.66 -0.1 187,388 + GFF877 0.89 -0.3 187,476 + -0.8 187,476 + +0.2 187,651 + GFF878 0.10 -0.4 187,691 - GFF878 0.21 +0.7 187,702 + GFF878 0.23 -0.1 187,702 + GFF878 0.23 +0.7 187,703 - GFF878 0.24 -1.1 187,750 + GFF878 0.36 +0.5 187,750 + GFF878 0.36 +0.2 187,751 - GFF878 0.36 -0.2 187,751 - GFF878 0.36 -0.4 187,753 + GFF878 0.36 +0.5 187,754 - GFF878 0.37 -0.2 187,754 - GFF878 0.37 -0.2 187,771 + GFF878 0.41 +0.0 187,772 - GFF878 0.41 -0.2 187,772 - GFF878 0.41 -0.1 187,780 + GFF878 0.43 -0.6 187,780 + GFF878 0.43 +0.3 187,781 - GFF878 0.44 -0.4 187,837 + GFF878 0.58 -1.1 187,837 + GFF878 0.58 -0.8 187,837 + GFF878 0.58 +0.3 187,837 + GFF878 0.58 +0.2 187,837 + GFF878 0.58 -0.0 187,838 - GFF878 0.58 -1.0 187,838 - GFF878 0.58 +0.3 187,838 - GFF878 0.58 -0.1 187,838 - GFF878 0.58 +0.9 188,212 + -1.4 188,212 + -0.2 188,228 - -0.3
Or see this region's nucleotide sequence