Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF83 and GFF84 are separated by 112 nucleotides GFF84 and GFF85 are separated by 131 nucleotides GFF85 and GFF86 are separated by 41 nucleotides GFF86 and GFF87 overlap by 4 nucleotides
GFF83 - Alkanal monooxygenase alpha chain (EC 1.14.14.3), at 99,554 to 100,615
GFF83
GFF84 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 100,728 to 101,444
GFF84
GFF85 - Transcriptional regulator, LysR family, at 101,576 to 102,475
GFF85
GFF86 - Cell morphology protein, at 102,517 to 103,191
GFF86
GFF87 - no description, at 103,188 to 103,868
GFF87
Position (kb) 101
102
103 Strain fitness (log2 ratio) -2
-1
0
1
2 at 100.695 kb on + strand at 100.695 kb on + strand at 100.695 kb on + strand at 100.695 kb on + strand at 100.696 kb on - strand at 100.757 kb on - strand at 101.110 kb on + strand, within GFF84 at 101.110 kb on + strand, within GFF84 at 101.111 kb on - strand, within GFF84 at 101.420 kb on - strand at 101.470 kb on + strand at 101.470 kb on + strand at 101.471 kb on - strand at 101.486 kb on + strand at 101.486 kb on + strand at 101.487 kb on - strand at 101.487 kb on - strand at 101.777 kb on + strand, within GFF85 at 101.777 kb on + strand, within GFF85 at 101.777 kb on + strand, within GFF85 at 101.777 kb on + strand, within GFF85 at 101.778 kb on - strand, within GFF85 at 101.778 kb on - strand, within GFF85 at 102.098 kb on + strand, within GFF85 at 102.099 kb on - strand, within GFF85 at 102.444 kb on + strand at 102.444 kb on + strand at 102.482 kb on + strand at 102.483 kb on - strand at 102.483 kb on - strand at 102.589 kb on + strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.670 kb on + strand, within GFF86 at 102.968 kb on - strand, within GFF86 at 102.980 kb on - strand, within GFF86 at 102.980 kb on - strand, within GFF86 at 103.273 kb on - strand, within GFF87 at 103.273 kb on - strand, within GFF87 at 103.405 kb on - strand, within GFF87
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 100,695 + -0.6 100,695 + -0.8 100,695 + -0.8 100,695 + -0.4 100,696 - +0.9 100,757 - -2.2 101,110 + GFF84 0.53 +0.6 101,110 + GFF84 0.53 -1.1 101,111 - GFF84 0.53 -0.1 101,420 - -1.8 101,470 + +0.4 101,470 + -0.5 101,471 - -0.1 101,486 + -0.1 101,486 + +0.2 101,487 - +0.4 101,487 - -0.2 101,777 + GFF85 0.22 -0.0 101,777 + GFF85 0.22 -2.5 101,777 + GFF85 0.22 -1.0 101,777 + GFF85 0.22 +2.7 101,778 - GFF85 0.22 -1.8 101,778 - GFF85 0.22 -1.3 102,098 + GFF85 0.58 -0.6 102,099 - GFF85 0.58 -0.3 102,444 + -1.0 102,444 + +0.3 102,482 + -0.5 102,483 - +0.2 102,483 - -0.1 102,589 + GFF86 0.11 -0.5 102,590 - GFF86 0.11 +0.9 102,590 - GFF86 0.11 -0.2 102,590 - GFF86 0.11 -1.2 102,590 - GFF86 0.11 +0.1 102,590 - GFF86 0.11 -0.5 102,670 + GFF86 0.23 +0.2 102,968 - GFF86 0.67 +0.7 102,980 - GFF86 0.69 +0.6 102,980 - GFF86 0.69 +1.5 103,273 - GFF87 0.12 -1.1 103,273 - GFF87 0.12 +1.4 103,405 - GFF87 0.32 +0.4
Or see this region's nucleotide sequence