Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6533 and GFF6534 are separated by 6 nucleotides GFF6534 and GFF6535 are separated by 39 nucleotides
GFF6533 - Methylphosphotriester-DNA--protein-cysteine S-methyltransferase (EC 2.1.1.n11) / DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 29,188 to 30,774
GFF6533
GFF6534 - Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 30,781 to 31,278
GFF6534
GFF6535 - Permease of the drug/metabolite transporter (DMT) superfamily, at 31,318 to 32,277
GFF6535
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 29.850 kb on + strand, within GFF6533 at 29.851 kb on + strand, within GFF6533 at 29.851 kb on + strand, within GFF6533 at 29.852 kb on - strand, within GFF6533 at 29.852 kb on - strand, within GFF6533 at 29.909 kb on + strand, within GFF6533 at 29.909 kb on + strand, within GFF6533 at 29.910 kb on - strand, within GFF6533 at 29.959 kb on + strand, within GFF6533 at 29.959 kb on + strand, within GFF6533 at 29.959 kb on + strand, within GFF6533 at 29.960 kb on - strand, within GFF6533 at 29.960 kb on - strand, within GFF6533 at 29.960 kb on - strand, within GFF6533 at 30.153 kb on + strand, within GFF6533 at 30.154 kb on - strand, within GFF6533 at 30.154 kb on - strand, within GFF6533 at 30.547 kb on + strand, within GFF6533 at 30.547 kb on + strand, within GFF6533 at 30.547 kb on + strand, within GFF6533 at 30.547 kb on + strand, within GFF6533 at 30.548 kb on - strand, within GFF6533 at 30.548 kb on - strand, within GFF6533 at 30.548 kb on - strand, within GFF6533 at 30.548 kb on - strand, within GFF6533 at 30.679 kb on + strand at 30.679 kb on + strand at 30.680 kb on - strand at 30.680 kb on - strand at 30.680 kb on - strand at 30.680 kb on - strand at 30.680 kb on - strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.806 kb on - strand at 30.806 kb on - strand at 30.806 kb on - strand at 30.806 kb on - strand at 30.806 kb on - strand at 31.090 kb on + strand, within GFF6534 at 31.090 kb on + strand, within GFF6534 at 31.090 kb on + strand, within GFF6534 at 31.090 kb on + strand, within GFF6534 at 31.091 kb on - strand, within GFF6534 at 31.091 kb on - strand, within GFF6534 at 31.277 kb on - strand at 31.277 kb on - strand at 31.870 kb on + strand, within GFF6535 at 31.871 kb on - strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.063 kb on - strand, within GFF6535
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 29,850 + GFF6533 0.42 -0.5 29,851 + GFF6533 0.42 -0.8 29,851 + GFF6533 0.42 -0.5 29,852 - GFF6533 0.42 -0.5 29,852 - GFF6533 0.42 -0.7 29,909 + GFF6533 0.45 -0.7 29,909 + GFF6533 0.45 -1.8 29,910 - GFF6533 0.45 -0.1 29,959 + GFF6533 0.49 -0.3 29,959 + GFF6533 0.49 +0.3 29,959 + GFF6533 0.49 +0.5 29,960 - GFF6533 0.49 +0.0 29,960 - GFF6533 0.49 -0.4 29,960 - GFF6533 0.49 -1.5 30,153 + GFF6533 0.61 -1.6 30,154 - GFF6533 0.61 -0.2 30,154 - GFF6533 0.61 -0.5 30,547 + GFF6533 0.86 -0.7 30,547 + GFF6533 0.86 +0.1 30,547 + GFF6533 0.86 -0.4 30,547 + GFF6533 0.86 -0.1 30,548 - GFF6533 0.86 -1.7 30,548 - GFF6533 0.86 +0.5 30,548 - GFF6533 0.86 -0.6 30,548 - GFF6533 0.86 +0.1 30,679 + +0.0 30,679 + -0.7 30,680 - +0.2 30,680 - -0.7 30,680 - -0.3 30,680 - -0.5 30,680 - +0.1 30,805 + +0.4 30,805 + +1.1 30,805 + +0.4 30,805 + -0.8 30,805 + +0.3 30,805 + -0.8 30,805 + -0.1 30,805 + +0.5 30,805 + -0.4 30,806 - +0.8 30,806 - -2.5 30,806 - -0.9 30,806 - -0.5 30,806 - -0.5 31,090 + GFF6534 0.62 -1.0 31,090 + GFF6534 0.62 -0.3 31,090 + GFF6534 0.62 -0.7 31,090 + GFF6534 0.62 -0.6 31,091 - GFF6534 0.62 +0.6 31,091 - GFF6534 0.62 -0.3 31,277 - -0.1 31,277 - +0.1 31,870 + GFF6535 0.57 +0.5 31,871 - GFF6535 0.58 -0.4 32,062 + GFF6535 0.78 -0.4 32,062 + GFF6535 0.78 -0.8 32,062 + GFF6535 0.78 -0.4 32,062 + GFF6535 0.78 -0.6 32,062 + GFF6535 0.78 +0.7 32,062 + GFF6535 0.78 -0.4 32,063 - GFF6535 0.78 +0.6 32,063 - GFF6535 0.78 +1.0 32,063 - GFF6535 0.78 +1.2 32,063 - GFF6535 0.78 +0.1 32,063 - GFF6535 0.78 -2.9
Or see this region's nucleotide sequence