Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6409 and GFF6410 are separated by 61 nucleotides GFF6410 and GFF6411 are separated by 11 nucleotides GFF6411 and GFF6412 overlap by 4 nucleotides
GFF6409 - Histidine ammonia-lyase (EC 4.3.1.3), at 83,649 to 85,208
GFF6409
GFF6410 - Histidine utilization repressor, at 85,270 to 86,007
GFF6410
GFF6411 - Histidine ABC transporter, ATP-binding protein, at 86,019 to 86,801
GFF6411
GFF6412 - Histidine ABC transporter, permease protein, at 86,798 to 87,640
GFF6412
Position (kb) 85
86
87 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 84.579 kb on + strand, within GFF6409 at 85.021 kb on + strand, within GFF6409 at 85.221 kb on - strand at 85.221 kb on - strand at 85.222 kb on + strand at 85.223 kb on - strand at 85.223 kb on - strand at 85.223 kb on - strand at 85.246 kb on + strand at 85.246 kb on + strand at 85.246 kb on + strand at 85.246 kb on + strand at 85.247 kb on - strand at 85.247 kb on - strand at 85.247 kb on - strand at 85.247 kb on - strand at 85.271 kb on + strand at 85.298 kb on + strand at 85.319 kb on + strand at 85.319 kb on + strand at 85.320 kb on - strand at 85.450 kb on + strand, within GFF6410 at 85.450 kb on + strand, within GFF6410 at 85.502 kb on + strand, within GFF6410 at 85.502 kb on + strand, within GFF6410 at 85.502 kb on + strand, within GFF6410 at 85.502 kb on + strand, within GFF6410 at 85.502 kb on + strand, within GFF6410 at 85.503 kb on - strand, within GFF6410 at 85.503 kb on - strand, within GFF6410 at 85.503 kb on - strand, within GFF6410 at 85.503 kb on - strand, within GFF6410 at 85.503 kb on - strand, within GFF6410 at 85.541 kb on + strand, within GFF6410 at 85.541 kb on + strand, within GFF6410 at 85.542 kb on - strand, within GFF6410 at 85.568 kb on + strand, within GFF6410 at 85.568 kb on + strand, within GFF6410 at 85.568 kb on + strand, within GFF6410 at 85.568 kb on + strand, within GFF6410 at 85.569 kb on - strand, within GFF6410 at 85.569 kb on - strand, within GFF6410 at 85.569 kb on - strand, within GFF6410 at 85.569 kb on - strand, within GFF6410 at 85.712 kb on + strand, within GFF6410 at 85.712 kb on + strand, within GFF6410 at 85.712 kb on + strand, within GFF6410 at 85.713 kb on - strand, within GFF6410 at 85.976 kb on + strand at 86.110 kb on + strand, within GFF6411 at 86.110 kb on + strand, within GFF6411 at 86.110 kb on + strand, within GFF6411 at 86.110 kb on + strand, within GFF6411 at 86.110 kb on + strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.543 kb on - strand, within GFF6411 at 86.543 kb on - strand, within GFF6411 at 86.543 kb on - strand, within GFF6411 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 84,579 + GFF6409 0.60 -0.2 85,021 + GFF6409 0.88 -0.2 85,221 - -0.4 85,221 - +0.2 85,222 + +0.0 85,223 - -1.5 85,223 - -0.6 85,223 - +0.9 85,246 + -0.4 85,246 + -0.0 85,246 + +0.6 85,246 + -0.1 85,247 - -0.4 85,247 - +1.0 85,247 - +0.1 85,247 - -0.4 85,271 + -0.2 85,298 + +2.4 85,319 + -0.8 85,319 + -0.4 85,320 - -0.2 85,450 + GFF6410 0.24 +0.2 85,450 + GFF6410 0.24 -1.1 85,502 + GFF6410 0.31 -0.8 85,502 + GFF6410 0.31 -2.0 85,502 + GFF6410 0.31 -1.5 85,502 + GFF6410 0.31 -1.3 85,502 + GFF6410 0.31 -1.0 85,503 - GFF6410 0.32 +0.2 85,503 - GFF6410 0.32 +0.3 85,503 - GFF6410 0.32 -0.8 85,503 - GFF6410 0.32 -1.3 85,503 - GFF6410 0.32 -2.7 85,541 + GFF6410 0.37 -0.9 85,541 + GFF6410 0.37 +3.4 85,542 - GFF6410 0.37 -2.5 85,568 + GFF6410 0.40 +2.3 85,568 + GFF6410 0.40 -0.1 85,568 + GFF6410 0.40 +0.4 85,568 + GFF6410 0.40 -0.8 85,569 - GFF6410 0.41 +0.2 85,569 - GFF6410 0.41 +0.0 85,569 - GFF6410 0.41 +0.5 85,569 - GFF6410 0.41 -0.2 85,712 + GFF6410 0.60 -0.8 85,712 + GFF6410 0.60 +0.2 85,712 + GFF6410 0.60 -0.4 85,713 - GFF6410 0.60 -0.9 85,976 + -0.2 86,110 + GFF6411 0.12 +1.0 86,110 + GFF6411 0.12 -1.9 86,110 + GFF6411 0.12 -0.7 86,110 + GFF6411 0.12 +0.6 86,110 + GFF6411 0.12 +1.1 86,111 - GFF6411 0.12 +0.0 86,111 - GFF6411 0.12 +0.3 86,111 - GFF6411 0.12 -0.0 86,111 - GFF6411 0.12 -0.3 86,111 - GFF6411 0.12 +1.8 86,111 - GFF6411 0.12 +1.5 86,542 + GFF6411 0.67 -2.6 86,542 + GFF6411 0.67 -1.1 86,542 + GFF6411 0.67 -0.2 86,542 + GFF6411 0.67 -1.0 86,542 + GFF6411 0.67 -0.7 86,543 - GFF6411 0.67 -0.7 86,543 - GFF6411 0.67 -1.0 86,543 - GFF6411 0.67 -0.5 86,982 + GFF6412 0.22 +0.2 86,982 + GFF6412 0.22 -0.1 86,982 + GFF6412 0.22 +0.3 86,982 + GFF6412 0.22 +0.3 86,982 + GFF6412 0.22 -0.3 86,982 + GFF6412 0.22 -0.3 86,982 + GFF6412 0.22 -1.3 86,982 + GFF6412 0.22 -0.8 86,983 - GFF6412 0.22 -0.3 86,983 - GFF6412 0.22 -0.2 86,983 - GFF6412 0.22 +0.2 86,983 - GFF6412 0.22 -0.1 86,983 - GFF6412 0.22 -0.8 86,983 - GFF6412 0.22 +0.2
Or see this region's nucleotide sequence