Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6361 and GFF6362 overlap by 4 nucleotides GFF6362 and GFF6363 overlap by 4 nucleotides GFF6363 and GFF6364 are separated by 7 nucleotides
GFF6361 - Pentapeptide repeat family protein, at 25,920 to 26,990
GFF6361
GFF6362 - Pentapeptide repeat family protein, at 26,987 to 29,590
GFF6362
GFF6363 - hypothetical protein, at 29,587 to 29,982
GFF6363
GFF6364 - VgrG protein, at 29,990 to 32,356
GFF6364
Position (kb) 26
27
28
29
30 Strain fitness (log2 ratio) -2
-1
0
1
2 at 27.043 kb on - strand at 27.076 kb on - strand at 27.237 kb on - strand at 27.578 kb on + strand, within GFF6362 at 27.826 kb on + strand, within GFF6362 at 28.047 kb on + strand, within GFF6362 at 28.048 kb on - strand, within GFF6362 at 28.938 kb on + strand, within GFF6362 at 28.998 kb on + strand, within GFF6362 at 28.998 kb on + strand, within GFF6362 at 28.998 kb on + strand, within GFF6362 at 29.269 kb on - strand, within GFF6362 at 29.358 kb on + strand at 29.359 kb on - strand at 29.359 kb on - strand at 29.359 kb on - strand at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.765 kb on + strand, within GFF6363 at 29.766 kb on - strand, within GFF6363 at 29.766 kb on - strand, within GFF6363 at 29.806 kb on + strand, within GFF6363 at 29.806 kb on + strand, within GFF6363 at 29.807 kb on - strand, within GFF6363 at 29.960 kb on + strand at 29.967 kb on - strand at 30.019 kb on - strand at 30.048 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 27,043 - -1.7 27,076 - +0.6 27,237 - +1.4 27,578 + GFF6362 0.23 +0.4 27,826 + GFF6362 0.32 -0.3 28,047 + GFF6362 0.41 -1.3 28,048 - GFF6362 0.41 +0.1 28,938 + GFF6362 0.75 +0.4 28,998 + GFF6362 0.77 +2.4 28,998 + GFF6362 0.77 +2.7 28,998 + GFF6362 0.77 -1.3 29,269 - GFF6362 0.88 -0.4 29,358 + -2.0 29,359 - -1.5 29,359 - -0.2 29,359 - -0.1 29,765 + GFF6363 0.45 -0.4 29,765 + GFF6363 0.45 +0.0 29,765 + GFF6363 0.45 -0.8 29,765 + GFF6363 0.45 -0.7 29,765 + GFF6363 0.45 -0.4 29,765 + GFF6363 0.45 -0.9 29,766 - GFF6363 0.45 +1.2 29,766 - GFF6363 0.45 +2.0 29,806 + GFF6363 0.55 +0.8 29,806 + GFF6363 0.55 -1.4 29,807 - GFF6363 0.56 +0.4 29,960 + +0.3 29,967 - -0.8 30,019 - -0.5 30,048 + -0.1
Or see this region's nucleotide sequence