Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6329 and GFF6330 are separated by 148 nucleotides GFF6330 and GFF6331 overlap by 8 nucleotides
GFF6329 - Leucyl-tRNA synthetase (EC 6.1.1.4), at 121,832 to 124,462
GFF6329
GFF6330 - BUG/TctC family periplasmic protein, at 124,611 to 125,585
GFF6330
GFF6331 - D-octopine dehydrogenase (EC 1.5.1.11), at 125,578 to 126,648
GFF6331
Position (kb)
124
125
126 Strain fitness (log2 ratio)
-2
-1
0
1 at 124.546 kb on - strand at 124.546 kb on - strand at 124.560 kb on + strand at 124.560 kb on + strand at 124.560 kb on + strand at 124.560 kb on + strand at 124.561 kb on - strand at 124.561 kb on - strand at 124.561 kb on - strand at 124.561 kb on - strand at 124.935 kb on + strand, within GFF6330 at 124.935 kb on + strand, within GFF6330 at 124.936 kb on - strand, within GFF6330 at 124.936 kb on - strand, within GFF6330 at 124.936 kb on - strand, within GFF6330 at 125.016 kb on + strand, within GFF6330 at 125.016 kb on + strand, within GFF6330 at 125.017 kb on - strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.583 kb on + strand at 125.584 kb on - strand at 125.584 kb on - strand at 125.584 kb on - strand at 125.593 kb on + strand at 125.593 kb on + strand at 125.594 kb on - strand at 125.594 kb on - strand at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 126.069 kb on + strand, within GFF6331 at 126.070 kb on - strand, within GFF6331 at 126.235 kb on + strand, within GFF6331 at 126.236 kb on - strand, within GFF6331 at 126.318 kb on - strand, within GFF6331 at 126.318 kb on - strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.469 kb on + strand, within GFF6331 at 126.469 kb on + strand, within GFF6331
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 124,546 - -0.2 124,546 - -1.5 124,560 + -0.4 124,560 + -0.6 124,560 + +0.6 124,560 + +0.2 124,561 - +0.9 124,561 - -0.1 124,561 - +0.5 124,561 - -0.4 124,935 + GFF6330 0.33 +0.6 124,935 + GFF6330 0.33 +0.5 124,936 - GFF6330 0.33 -0.1 124,936 - GFF6330 0.33 +0.2 124,936 - GFF6330 0.33 -0.4 125,016 + GFF6330 0.42 -0.3 125,016 + GFF6330 0.42 +1.2 125,017 - GFF6330 0.42 -0.6 125,073 + GFF6330 0.47 +0.1 125,073 + GFF6330 0.47 -0.3 125,073 + GFF6330 0.47 +0.9 125,073 + GFF6330 0.47 -0.3 125,073 + GFF6330 0.47 -0.8 125,073 + GFF6330 0.47 +0.4 125,074 - GFF6330 0.47 +0.0 125,074 - GFF6330 0.47 +0.7 125,074 - GFF6330 0.47 -0.2 125,074 - GFF6330 0.47 -0.2 125,074 - GFF6330 0.47 +0.1 125,074 - GFF6330 0.47 -0.1 125,074 - GFF6330 0.47 -0.6 125,583 + -0.3 125,584 - -1.1 125,584 - -0.5 125,584 - -2.1 125,593 + -0.3 125,593 + -0.2 125,594 - +0.0 125,594 - -0.2 125,807 + GFF6331 0.21 -0.8 125,807 + GFF6331 0.21 -0.5 125,807 + GFF6331 0.21 -0.5 125,807 + GFF6331 0.21 -0.4 125,807 + GFF6331 0.21 -0.4 125,807 + GFF6331 0.21 -0.0 125,807 + GFF6331 0.21 -0.8 125,807 + GFF6331 0.21 -0.4 125,808 - GFF6331 0.21 -0.3 125,808 - GFF6331 0.21 +1.7 125,808 - GFF6331 0.21 -0.5 125,808 - GFF6331 0.21 -1.4 125,808 - GFF6331 0.21 +1.7 125,808 - GFF6331 0.21 -0.1 125,808 - GFF6331 0.21 +0.4 125,920 + GFF6331 0.32 +0.7 125,920 + GFF6331 0.32 -0.6 125,920 + GFF6331 0.32 -2.1 125,920 + GFF6331 0.32 -0.5 125,920 + GFF6331 0.32 -0.7 125,921 - GFF6331 0.32 +0.4 125,921 - GFF6331 0.32 +0.2 125,921 - GFF6331 0.32 -0.2 125,921 - GFF6331 0.32 -0.0 125,921 - GFF6331 0.32 +0.3 126,069 + GFF6331 0.46 -0.8 126,070 - GFF6331 0.46 +0.0 126,235 + GFF6331 0.61 +1.5 126,236 - GFF6331 0.61 +0.1 126,318 - GFF6331 0.69 +0.7 126,318 - GFF6331 0.69 +1.7 126,448 + GFF6331 0.81 -0.3 126,448 + GFF6331 0.81 +0.5 126,448 + GFF6331 0.81 -0.6 126,449 - GFF6331 0.81 +1.7 126,449 - GFF6331 0.81 -0.2 126,449 - GFF6331 0.81 +0.2 126,449 - GFF6331 0.81 -0.2 126,469 + GFF6331 0.83 +0.7 126,469 + GFF6331 0.83 -0.6
Or see this region's nucleotide sequence