Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5888 and GFF5889 are separated by 534 nucleotides GFF5889 and GFF5890 overlap by 4 nucleotides GFF5890 and GFF5891 are separated by 31 nucleotides
GFF5888 - Translation elongation factor Tu, at 107,144 to 108,337
GFF5888
GFF5889 - Uracil-DNA glycosylase, family 1 (EC 3.2.2.27), at 108,872 to 109,579
GFF5889
GFF5890 - Indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 109,576 to 110,376
GFF5890
GFF5891 - Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 110,408 to 111,430
GFF5891
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-2
-1
0
1 at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.369 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.496 kb on - strand at 108.643 kb on - strand at 108.643 kb on - strand at 108.659 kb on - strand at 108.659 kb on - strand at 108.659 kb on - strand at 108.659 kb on - strand at 108.659 kb on - strand at 108.797 kb on - strand at 108.821 kb on - strand at 108.994 kb on + strand, within GFF5889 at 108.994 kb on + strand, within GFF5889 at 108.994 kb on + strand, within GFF5889 at 108.995 kb on - strand, within GFF5889 at 108.995 kb on - strand, within GFF5889 at 108.995 kb on - strand, within GFF5889 at 109.025 kb on - strand, within GFF5889 at 109.025 kb on - strand, within GFF5889 at 109.240 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.256 kb on + strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.257 kb on - strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.270 kb on + strand, within GFF5889 at 109.271 kb on - strand, within GFF5889 at 109.271 kb on - strand, within GFF5889 at 109.347 kb on + strand, within GFF5889 at 109.348 kb on - strand, within GFF5889 at 109.393 kb on + strand, within GFF5889 at 109.394 kb on - strand, within GFF5889 at 109.394 kb on - strand, within GFF5889 at 109.394 kb on - strand, within GFF5889 at 110.377 kb on - strand at 110.377 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 108,369 - -1.7 108,369 - -0.9 108,369 - -0.1 108,369 - -1.6 108,369 - +0.1 108,369 - -1.1 108,369 - +0.4 108,369 - +0.1 108,369 - +0.6 108,496 - -0.7 108,496 - -0.3 108,496 - -1.0 108,496 - -0.1 108,496 - -1.1 108,496 - -0.9 108,496 - -0.9 108,643 - +0.2 108,643 - +0.4 108,659 - -0.9 108,659 - -0.0 108,659 - -0.9 108,659 - +0.4 108,659 - -0.5 108,797 - +0.6 108,821 - -0.3 108,994 + GFF5889 0.17 +1.1 108,994 + GFF5889 0.17 +0.4 108,994 + GFF5889 0.17 +0.4 108,995 - GFF5889 0.17 +0.2 108,995 - GFF5889 0.17 +0.9 108,995 - GFF5889 0.17 +0.3 109,025 - GFF5889 0.22 -2.0 109,025 - GFF5889 0.22 -0.4 109,240 + GFF5889 0.52 -0.7 109,256 + GFF5889 0.54 +0.4 109,256 + GFF5889 0.54 -1.4 109,256 + GFF5889 0.54 -0.1 109,256 + GFF5889 0.54 -0.8 109,256 + GFF5889 0.54 -0.2 109,256 + GFF5889 0.54 -0.3 109,256 + GFF5889 0.54 -0.9 109,256 + GFF5889 0.54 -1.0 109,256 + GFF5889 0.54 -1.2 109,256 + GFF5889 0.54 -0.1 109,257 - GFF5889 0.54 -0.1 109,257 - GFF5889 0.54 -0.3 109,257 - GFF5889 0.54 -0.4 109,257 - GFF5889 0.54 -0.3 109,257 - GFF5889 0.54 -0.3 109,270 + GFF5889 0.56 -0.8 109,270 + GFF5889 0.56 -1.5 109,270 + GFF5889 0.56 -0.3 109,270 + GFF5889 0.56 -1.3 109,270 + GFF5889 0.56 -0.7 109,270 + GFF5889 0.56 -0.6 109,271 - GFF5889 0.56 -1.7 109,271 - GFF5889 0.56 -0.9 109,347 + GFF5889 0.67 +0.3 109,348 - GFF5889 0.67 -0.6 109,393 + GFF5889 0.74 -0.0 109,394 - GFF5889 0.74 +0.3 109,394 - GFF5889 0.74 -0.9 109,394 - GFF5889 0.74 +0.1 110,377 - +0.3 110,377 - +0.1
Or see this region's nucleotide sequence