Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5712 and GFF5713 are separated by 91 nucleotides GFF5713 and GFF5714 are separated by 72 nucleotides GFF5714 and GFF5715 are separated by 28 nucleotides GFF5715 and GFF5716 are separated by 3 nucleotides GFF5716 and GFF5717 are separated by 22 nucleotides
GFF5712 - no description, at 84,608 to 84,916
GFF5712
GFF5713 - Flagellar basal-body P-ring formation protein FlgA, at 85,008 to 85,724
GFF5713
GFF5714 - no description, at 85,797 to 85,916
GFF5714
GFF5715 - Flagellar basal-body rod protein FlgB, at 85,945 to 86,352
GFF5715
GFF5716 - Flagellar basal-body rod protein FlgC, at 86,356 to 86,772
GFF5716
GFF5717 - Flagellar basal-body rod modification protein FlgD, at 86,795 to 87,475
GFF5717
Position (kb)
85
86 Strain fitness (log2 ratio)
-2
-1
0
1 at 84.941 kb on + strand at 84.941 kb on + strand at 84.942 kb on - strand at 84.942 kb on - strand at 84.943 kb on + strand at 84.944 kb on - strand at 84.944 kb on - strand at 84.944 kb on - strand at 84.944 kb on - strand at 85.009 kb on + strand at 85.009 kb on + strand at 85.010 kb on - strand at 85.378 kb on + strand, within GFF5713 at 85.378 kb on + strand, within GFF5713 at 85.378 kb on + strand, within GFF5713 at 85.379 kb on - strand, within GFF5713 at 85.379 kb on - strand, within GFF5713 at 85.379 kb on - strand, within GFF5713 at 85.379 kb on - strand, within GFF5713 at 85.588 kb on + strand, within GFF5713 at 85.588 kb on + strand, within GFF5713 at 85.588 kb on + strand, within GFF5713 at 85.880 kb on + strand, within GFF5714 at 85.880 kb on + strand, within GFF5714 at 85.880 kb on + strand, within GFF5714 at 85.880 kb on + strand, within GFF5714 at 85.881 kb on - strand, within GFF5714 at 85.881 kb on - strand, within GFF5714 at 85.881 kb on - strand, within GFF5714 at 86.031 kb on + strand, within GFF5715 at 86.056 kb on + strand, within GFF5715 at 86.056 kb on + strand, within GFF5715 at 86.056 kb on + strand, within GFF5715 at 86.057 kb on - strand, within GFF5715 at 86.057 kb on - strand, within GFF5715 at 86.057 kb on - strand, within GFF5715 at 86.057 kb on - strand, within GFF5715 at 86.057 kb on - strand, within GFF5715 at 86.201 kb on - strand, within GFF5715 at 86.287 kb on + strand, within GFF5715 at 86.351 kb on - strand at 86.351 kb on - strand at 86.635 kb on + strand, within GFF5716 at 86.635 kb on + strand, within GFF5716 at 86.635 kb on + strand, within GFF5716 at 86.635 kb on + strand, within GFF5716 at 86.635 kb on + strand, within GFF5716 at 86.635 kb on + strand, within GFF5716 at 86.635 kb on + strand, within GFF5716 at 86.636 kb on - strand, within GFF5716 at 86.636 kb on - strand, within GFF5716 at 86.636 kb on - strand, within GFF5716 at 86.701 kb on + strand, within GFF5716 at 86.701 kb on + strand, within GFF5716
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 84,941 + -0.8 84,941 + +0.7 84,942 - +0.2 84,942 - -0.7 84,943 + -0.0 84,944 - -0.2 84,944 - -0.7 84,944 - -0.5 84,944 - -0.4 85,009 + -0.7 85,009 + +0.4 85,010 - -1.1 85,378 + GFF5713 0.52 -0.2 85,378 + GFF5713 0.52 -2.6 85,378 + GFF5713 0.52 +0.8 85,379 - GFF5713 0.52 +1.3 85,379 - GFF5713 0.52 -0.3 85,379 - GFF5713 0.52 -0.1 85,379 - GFF5713 0.52 +1.7 85,588 + GFF5713 0.81 -0.1 85,588 + GFF5713 0.81 -1.3 85,588 + GFF5713 0.81 +0.4 85,880 + GFF5714 0.69 -0.5 85,880 + GFF5714 0.69 -0.4 85,880 + GFF5714 0.69 -0.8 85,880 + GFF5714 0.69 -0.9 85,881 - GFF5714 0.70 +0.1 85,881 - GFF5714 0.70 -0.3 85,881 - GFF5714 0.70 -1.2 86,031 + GFF5715 0.21 +0.9 86,056 + GFF5715 0.27 -0.9 86,056 + GFF5715 0.27 +1.7 86,056 + GFF5715 0.27 -1.0 86,057 - GFF5715 0.27 -0.6 86,057 - GFF5715 0.27 -0.9 86,057 - GFF5715 0.27 -0.3 86,057 - GFF5715 0.27 +0.2 86,057 - GFF5715 0.27 -0.2 86,201 - GFF5715 0.63 -0.1 86,287 + GFF5715 0.84 -0.2 86,351 - -0.1 86,351 - -0.1 86,635 + GFF5716 0.67 -0.2 86,635 + GFF5716 0.67 -0.3 86,635 + GFF5716 0.67 -0.1 86,635 + GFF5716 0.67 +0.5 86,635 + GFF5716 0.67 -0.8 86,635 + GFF5716 0.67 -0.3 86,635 + GFF5716 0.67 -0.5 86,636 - GFF5716 0.67 +0.3 86,636 - GFF5716 0.67 -0.0 86,636 - GFF5716 0.67 +0.7 86,701 + GFF5716 0.83 +1.7 86,701 + GFF5716 0.83 -0.5
Or see this region's nucleotide sequence