Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5690 and GFF5691 overlap by 4 nucleotides GFF5691 and GFF5692 are separated by 157 nucleotides GFF5692 and GFF5693 are separated by 161 nucleotides
GFF5690 - Glutamate/aspartate ABC transporter, ATP-binding protein GltL (TC 3.A.1.3.4), at 65,319 to 66,062
GFF5690
GFF5691 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 66,059 to 66,769
GFF5691
GFF5692 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 66,927 to 67,622
GFF5692
GFF5693 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 67,784 to 68,611
GFF5693
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 66.023 kb on - strand at 66.023 kb on - strand at 66.031 kb on + strand at 66.031 kb on + strand at 66.123 kb on + strand at 66.124 kb on - strand at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.173 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.364 kb on - strand, within GFF5691 at 66.528 kb on + strand, within GFF5691 at 66.528 kb on + strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.738 kb on + strand at 66.738 kb on + strand at 66.738 kb on + strand at 66.738 kb on + strand at 66.739 kb on - strand at 66.747 kb on + strand at 66.958 kb on + strand at 66.958 kb on + strand at 66.958 kb on + strand at 66.959 kb on - strand at 66.959 kb on - strand at 67.000 kb on + strand, within GFF5692 at 67.000 kb on + strand, within GFF5692 at 67.000 kb on + strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.069 kb on + strand, within GFF5692 at 67.069 kb on + strand, within GFF5692 at 67.069 kb on + strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.179 kb on + strand, within GFF5692 at 67.202 kb on - strand, within GFF5692 at 67.295 kb on - strand, within GFF5692 at 67.342 kb on + strand, within GFF5692 at 67.343 kb on - strand, within GFF5692 at 67.343 kb on - strand, within GFF5692 at 67.343 kb on - strand, within GFF5692 at 67.343 kb on - strand, within GFF5692 at 67.441 kb on + strand, within GFF5692 at 67.441 kb on + strand, within GFF5692 at 67.442 kb on - strand, within GFF5692 at 67.582 kb on + strand at 67.582 kb on + strand at 67.582 kb on + strand at 67.583 kb on - strand at 67.583 kb on - strand at 67.606 kb on + strand at 67.607 kb on - strand at 67.635 kb on + strand at 67.635 kb on + strand at 67.635 kb on + strand at 67.636 kb on - strand at 67.636 kb on - strand at 67.636 kb on - strand at 67.636 kb on - strand at 67.653 kb on + strand at 67.653 kb on + strand at 67.654 kb on - strand at 67.851 kb on + strand at 67.852 kb on - strand at 67.995 kb on + strand, within GFF5693 at 67.995 kb on + strand, within GFF5693 at 67.995 kb on + strand, within GFF5693 at 67.995 kb on + strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 68.026 kb on - strand, within GFF5693 at 68.058 kb on + strand, within GFF5693 at 68.058 kb on + strand, within GFF5693 at 68.058 kb on + strand, within GFF5693 at 68.059 kb on - strand, within GFF5693
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 66,023 - -0.4 66,023 - +0.3 66,031 + -1.2 66,031 + -1.6 66,123 + -0.2 66,124 - -0.6 66,159 + GFF5691 0.14 +1.1 66,159 + GFF5691 0.14 +0.0 66,159 + GFF5691 0.14 +0.2 66,159 + GFF5691 0.14 -1.9 66,160 - GFF5691 0.14 -1.1 66,160 - GFF5691 0.14 +0.2 66,160 - GFF5691 0.14 +0.3 66,173 + GFF5691 0.16 +1.0 66,180 + GFF5691 0.17 -0.5 66,180 + GFF5691 0.17 -0.8 66,180 + GFF5691 0.17 -0.4 66,180 + GFF5691 0.17 -2.0 66,180 + GFF5691 0.17 -0.4 66,181 - GFF5691 0.17 +0.0 66,181 - GFF5691 0.17 -0.7 66,181 - GFF5691 0.17 -1.3 66,181 - GFF5691 0.17 +0.1 66,181 - GFF5691 0.17 -0.2 66,181 - GFF5691 0.17 +0.4 66,258 + GFF5691 0.28 +2.4 66,258 + GFF5691 0.28 -1.2 66,258 + GFF5691 0.28 -0.2 66,258 + GFF5691 0.28 -0.1 66,258 + GFF5691 0.28 -0.2 66,259 - GFF5691 0.28 -1.5 66,259 - GFF5691 0.28 +0.5 66,259 - GFF5691 0.28 +0.0 66,259 - GFF5691 0.28 -0.2 66,364 - GFF5691 0.43 +0.5 66,528 + GFF5691 0.66 +0.5 66,528 + GFF5691 0.66 -0.3 66,529 - GFF5691 0.66 -0.9 66,529 - GFF5691 0.66 -2.0 66,529 - GFF5691 0.66 +0.7 66,738 + -0.1 66,738 + +0.1 66,738 + -0.3 66,738 + +0.2 66,739 - +0.7 66,747 + +0.4 66,958 + +0.7 66,958 + +0.9 66,958 + +0.4 66,959 - -0.8 66,959 - -0.3 67,000 + GFF5692 0.10 -1.0 67,000 + GFF5692 0.10 +1.0 67,000 + GFF5692 0.10 -0.5 67,001 - GFF5692 0.11 -0.6 67,001 - GFF5692 0.11 -0.2 67,001 - GFF5692 0.11 +1.1 67,001 - GFF5692 0.11 -0.5 67,069 + GFF5692 0.20 +0.7 67,069 + GFF5692 0.20 -0.1 67,069 + GFF5692 0.20 -0.4 67,070 - GFF5692 0.21 -1.1 67,070 - GFF5692 0.21 -2.0 67,070 - GFF5692 0.21 -1.3 67,070 - GFF5692 0.21 -0.2 67,070 - GFF5692 0.21 +0.1 67,070 - GFF5692 0.21 +3.0 67,179 + GFF5692 0.36 +0.1 67,202 - GFF5692 0.40 +1.0 67,295 - GFF5692 0.53 +0.7 67,342 + GFF5692 0.60 +0.6 67,343 - GFF5692 0.60 +0.8 67,343 - GFF5692 0.60 +0.7 67,343 - GFF5692 0.60 -0.1 67,343 - GFF5692 0.60 -1.3 67,441 + GFF5692 0.74 -1.0 67,441 + GFF5692 0.74 +0.5 67,442 - GFF5692 0.74 -0.4 67,582 + -0.0 67,582 + +0.5 67,582 + -0.8 67,583 - +0.2 67,583 - +0.3 67,606 + +0.1 67,607 - +0.4 67,635 + -0.2 67,635 + -0.6 67,635 + -0.9 67,636 - +0.5 67,636 - -0.7 67,636 - -0.1 67,636 - +0.0 67,653 + -0.1 67,653 + -0.3 67,654 - -0.3 67,851 + +0.3 67,852 - -0.4 67,995 + GFF5693 0.25 -0.5 67,995 + GFF5693 0.25 +1.0 67,995 + GFF5693 0.25 +0.7 67,995 + GFF5693 0.25 -0.4 67,996 - GFF5693 0.26 -1.2 67,996 - GFF5693 0.26 -0.0 67,996 - GFF5693 0.26 -0.1 67,996 - GFF5693 0.26 +0.5 67,996 - GFF5693 0.26 -0.3 67,996 - GFF5693 0.26 -0.6 68,026 - GFF5693 0.29 -0.4 68,058 + GFF5693 0.33 -0.3 68,058 + GFF5693 0.33 -0.8 68,058 + GFF5693 0.33 +0.4 68,059 - GFF5693 0.33 +0.1
Or see this region's nucleotide sequence