Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5511 and GFF5512 are separated by 36 nucleotides GFF5512 and GFF5513 overlap by 4 nucleotides GFF5513 and GFF5514 overlap by 4 nucleotides
GFF5511 - FIG000875: Thioredoxin domain-containing protein EC-YbbN, at 141,849 to 142,769
GFF5511
GFF5512 - N5-carboxyaminoimidazole ribonucleotide mutase (EC 5.4.99.18), at 142,806 to 143,360
GFF5512
GFF5513 - N5-carboxyaminoimidazole ribonucleotide synthase (EC 6.3.4.18), at 143,357 to 144,547
GFF5513
GFF5514 - Threonylcarbamoyl-AMP synthase (EC 2.7.7.87) / SUA5 domain with internal deletion, at 144,544 to 145,545
GFF5514
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 142.627 kb on + strand, within GFF5511 at 142.627 kb on + strand, within GFF5511 at 142.628 kb on - strand, within GFF5511 at 142.628 kb on - strand, within GFF5511 at 142.793 kb on + strand at 142.793 kb on + strand at 142.794 kb on - strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.956 kb on + strand, within GFF5512 at 142.957 kb on - strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.110 kb on - strand, within GFF5512 at 143.110 kb on - strand, within GFF5512 at 143.459 kb on + strand at 143.459 kb on + strand at 143.534 kb on + strand, within GFF5513 at 143.534 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.200 kb on + strand, within GFF5513 at 144.200 kb on + strand, within GFF5513 at 144.404 kb on + strand, within GFF5513 at 144.404 kb on + strand, within GFF5513 at 144.404 kb on + strand, within GFF5513 at 144.405 kb on - strand, within GFF5513
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 142,627 + GFF5511 0.84 +0.5 142,627 + GFF5511 0.84 +0.7 142,628 - GFF5511 0.85 +0.0 142,628 - GFF5511 0.85 -0.8 142,793 + +0.1 142,793 + +0.4 142,794 - -0.5 142,831 + +0.8 142,831 + +0.8 142,831 + -0.2 142,831 + +0.2 142,831 + -1.2 142,832 - +1.8 142,832 - +0.2 142,832 - +0.8 142,832 - +0.2 142,832 - -1.4 142,832 - +0.8 142,832 - -0.2 142,956 + GFF5512 0.27 +0.2 142,957 - GFF5512 0.27 -2.0 143,109 + GFF5512 0.55 -0.8 143,109 + GFF5512 0.55 -2.2 143,109 + GFF5512 0.55 -0.8 143,109 + GFF5512 0.55 -1.4 143,109 + GFF5512 0.55 -2.3 143,109 + GFF5512 0.55 -2.6 143,109 + GFF5512 0.55 -2.0 143,109 + GFF5512 0.55 -0.5 143,110 - GFF5512 0.55 +0.2 143,110 - GFF5512 0.55 +0.2 143,459 + -1.5 143,459 + -0.5 143,534 + GFF5513 0.15 -1.5 143,534 + GFF5513 0.15 +1.8 144,107 + GFF5513 0.63 -0.2 144,107 + GFF5513 0.63 -1.2 144,107 + GFF5513 0.63 -1.7 144,107 + GFF5513 0.63 -0.9 144,107 + GFF5513 0.63 -0.2 144,200 + GFF5513 0.71 -1.5 144,200 + GFF5513 0.71 -0.5 144,404 + GFF5513 0.88 -1.0 144,404 + GFF5513 0.88 -1.7 144,404 + GFF5513 0.88 -0.2 144,405 - GFF5513 0.88 -1.5
Or see this region's nucleotide sequence