Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5366 and GFF5367 overlap by 4 nucleotides GFF5367 and GFF5368 overlap by 4 nucleotides
GFF5366 - L-Proline/Glycine betaine transporter ProP, at 239,159 to 240,511
GFF5366
GFF5367 - GTP cyclohydrolase 1 type 2 homolog YbgI, at 240,508 to 241,281
GFF5367
GFF5368 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 241,278 to 242,321
GFF5368
Position (kb)
240
241
242 Strain fitness (log2 ratio)
-2
-1
0
1 at 239.516 kb on + strand, within GFF5366 at 239.516 kb on + strand, within GFF5366 at 239.516 kb on + strand, within GFF5366 at 239.516 kb on + strand, within GFF5366 at 239.516 kb on + strand, within GFF5366 at 239.517 kb on - strand, within GFF5366 at 239.517 kb on - strand, within GFF5366 at 239.517 kb on - strand, within GFF5366 at 239.517 kb on - strand, within GFF5366 at 239.729 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.807 kb on + strand, within GFF5366 at 239.808 kb on - strand, within GFF5366 at 239.808 kb on - strand, within GFF5366 at 239.808 kb on - strand, within GFF5366 at 239.924 kb on + strand, within GFF5366 at 239.925 kb on - strand, within GFF5366 at 239.925 kb on - strand, within GFF5366 at 239.925 kb on - strand, within GFF5366 at 239.978 kb on + strand, within GFF5366 at 239.978 kb on + strand, within GFF5366 at 239.979 kb on - strand, within GFF5366 at 239.979 kb on - strand, within GFF5366 at 239.979 kb on - strand, within GFF5366 at 239.990 kb on + strand, within GFF5366 at 239.990 kb on + strand, within GFF5366 at 239.991 kb on - strand, within GFF5366 at 239.991 kb on - strand, within GFF5366 at 239.991 kb on - strand, within GFF5366 at 239.991 kb on - strand, within GFF5366 at 239.991 kb on - strand, within GFF5366 at 240.155 kb on + strand, within GFF5366 at 240.155 kb on + strand, within GFF5366 at 240.164 kb on + strand, within GFF5366 at 240.164 kb on + strand, within GFF5366 at 240.164 kb on + strand, within GFF5366 at 240.164 kb on + strand, within GFF5366 at 240.165 kb on - strand, within GFF5366 at 240.206 kb on + strand, within GFF5366 at 240.206 kb on + strand, within GFF5366 at 240.308 kb on + strand, within GFF5366 at 240.308 kb on + strand, within GFF5366 at 240.308 kb on + strand, within GFF5366 at 240.308 kb on + strand, within GFF5366 at 240.308 kb on + strand, within GFF5366 at 240.308 kb on + strand, within GFF5366 at 240.309 kb on - strand, within GFF5366 at 240.309 kb on - strand, within GFF5366 at 240.309 kb on - strand, within GFF5366 at 240.309 kb on - strand, within GFF5366 at 240.309 kb on - strand, within GFF5366 at 240.320 kb on + strand, within GFF5366 at 240.320 kb on + strand, within GFF5366 at 240.320 kb on + strand, within GFF5366 at 240.320 kb on + strand, within GFF5366 at 240.320 kb on + strand, within GFF5366 at 240.320 kb on + strand, within GFF5366 at 240.321 kb on - strand, within GFF5366 at 240.321 kb on - strand, within GFF5366 at 240.321 kb on - strand, within GFF5366 at 240.321 kb on - strand, within GFF5366 at 240.321 kb on - strand, within GFF5366 at 240.395 kb on + strand at 240.395 kb on + strand at 240.395 kb on + strand at 240.396 kb on - strand at 240.396 kb on - strand at 240.578 kb on - strand at 240.799 kb on + strand, within GFF5367 at 241.069 kb on + strand, within GFF5367 at 241.070 kb on - strand, within GFF5367 at 241.070 kb on - strand, within GFF5367 at 241.070 kb on - strand, within GFF5367 at 241.070 kb on - strand, within GFF5367 at 241.070 kb on - strand, within GFF5367 at 241.141 kb on + strand, within GFF5367 at 241.199 kb on - strand, within GFF5367
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 239,516 + GFF5366 0.26 +1.0 239,516 + GFF5366 0.26 -0.2 239,516 + GFF5366 0.26 +0.1 239,516 + GFF5366 0.26 +1.3 239,516 + GFF5366 0.26 +0.1 239,517 - GFF5366 0.26 -0.2 239,517 - GFF5366 0.26 +1.2 239,517 - GFF5366 0.26 -1.8 239,517 - GFF5366 0.26 -0.3 239,729 + GFF5366 0.42 +0.8 239,807 + GFF5366 0.48 +1.0 239,807 + GFF5366 0.48 +0.0 239,807 + GFF5366 0.48 +0.0 239,807 + GFF5366 0.48 +0.0 239,807 + GFF5366 0.48 +0.0 239,807 + GFF5366 0.48 +0.6 239,807 + GFF5366 0.48 -0.7 239,808 - GFF5366 0.48 +0.5 239,808 - GFF5366 0.48 -0.2 239,808 - GFF5366 0.48 +0.5 239,924 + GFF5366 0.57 -0.6 239,925 - GFF5366 0.57 +0.1 239,925 - GFF5366 0.57 +1.0 239,925 - GFF5366 0.57 +0.1 239,978 + GFF5366 0.61 +0.7 239,978 + GFF5366 0.61 -1.0 239,979 - GFF5366 0.61 -0.9 239,979 - GFF5366 0.61 -0.0 239,979 - GFF5366 0.61 -0.2 239,990 + GFF5366 0.61 +0.5 239,990 + GFF5366 0.61 -1.3 239,991 - GFF5366 0.61 +1.4 239,991 - GFF5366 0.61 +1.5 239,991 - GFF5366 0.61 +0.2 239,991 - GFF5366 0.61 +0.2 239,991 - GFF5366 0.61 -0.9 240,155 + GFF5366 0.74 +0.4 240,155 + GFF5366 0.74 -0.3 240,164 + GFF5366 0.74 +0.1 240,164 + GFF5366 0.74 -1.2 240,164 + GFF5366 0.74 +0.3 240,164 + GFF5366 0.74 +0.7 240,165 - GFF5366 0.74 -0.9 240,206 + GFF5366 0.77 +0.5 240,206 + GFF5366 0.77 +0.2 240,308 + GFF5366 0.85 -0.6 240,308 + GFF5366 0.85 -0.2 240,308 + GFF5366 0.85 -0.3 240,308 + GFF5366 0.85 -2.7 240,308 + GFF5366 0.85 -2.7 240,308 + GFF5366 0.85 +1.3 240,309 - GFF5366 0.85 -1.6 240,309 - GFF5366 0.85 -1.4 240,309 - GFF5366 0.85 +0.8 240,309 - GFF5366 0.85 +0.0 240,309 - GFF5366 0.85 -1.1 240,320 + GFF5366 0.86 +0.3 240,320 + GFF5366 0.86 -1.1 240,320 + GFF5366 0.86 +0.7 240,320 + GFF5366 0.86 +0.1 240,320 + GFF5366 0.86 +0.0 240,320 + GFF5366 0.86 -0.0 240,321 - GFF5366 0.86 -0.2 240,321 - GFF5366 0.86 -0.3 240,321 - GFF5366 0.86 +1.2 240,321 - GFF5366 0.86 -0.7 240,321 - GFF5366 0.86 -0.2 240,395 + -0.3 240,395 + -0.6 240,395 + -0.2 240,396 - -0.1 240,396 - +1.5 240,578 - +1.7 240,799 + GFF5367 0.38 +0.1 241,069 + GFF5367 0.72 -0.9 241,070 - GFF5367 0.73 +1.0 241,070 - GFF5367 0.73 +1.0 241,070 - GFF5367 0.73 +1.4 241,070 - GFF5367 0.73 +1.5 241,070 - GFF5367 0.73 +1.2 241,141 + GFF5367 0.82 -0.4 241,199 - GFF5367 0.89 -0.3
Or see this region's nucleotide sequence