Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5354 and GFF5355 are separated by 60 nucleotides GFF5355 and GFF5356 are separated by 7 nucleotides GFF5356 and GFF5357 are separated by 19 nucleotides GFF5357 and GFF5358 are separated by 37 nucleotides
GFF5354 - C4-dicarboxylate transport protein, at 231,264 to 232,571
GFF5354
GFF5355 - no description, at 232,632 to 232,772
GFF5355
GFF5356 - Aldose 1-epimerase, at 232,780 to 233,538
GFF5356
GFF5357 - Imidazole glycerol phosphate synthase cyclase subunit, at 233,558 to 234,337
GFF5357
GFF5358 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19), at 234,375 to 234,764
GFF5358
Position (kb) 232
233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 231.880 kb on + strand, within GFF5354 at 231.880 kb on + strand, within GFF5354 at 231.880 kb on + strand, within GFF5354 at 231.880 kb on + strand, within GFF5354 at 231.880 kb on + strand, within GFF5354 at 231.881 kb on - strand, within GFF5354 at 231.881 kb on - strand, within GFF5354 at 231.881 kb on - strand, within GFF5354 at 231.881 kb on - strand, within GFF5354 at 231.881 kb on - strand, within GFF5354 at 231.988 kb on + strand, within GFF5354 at 231.989 kb on - strand, within GFF5354 at 232.450 kb on + strand at 232.450 kb on + strand at 232.451 kb on - strand at 232.451 kb on - strand at 232.451 kb on - strand at 232.504 kb on + strand at 232.504 kb on + strand at 232.504 kb on + strand at 232.504 kb on + strand at 232.504 kb on + strand at 232.504 kb on + strand at 232.504 kb on + strand at 232.504 kb on + strand at 232.505 kb on - strand at 232.505 kb on - strand at 232.505 kb on - strand at 232.505 kb on - strand at 232.505 kb on - strand at 232.505 kb on - strand at 232.575 kb on + strand at 232.576 kb on - strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.593 kb on - strand at 232.593 kb on - strand at 232.593 kb on - strand at 232.593 kb on - strand at 232.593 kb on - strand at 232.598 kb on + strand at 232.598 kb on + strand at 232.598 kb on + strand at 232.598 kb on + strand at 232.598 kb on + strand at 232.598 kb on + strand at 232.598 kb on + strand at 232.599 kb on - strand at 232.599 kb on - strand at 232.599 kb on - strand at 232.599 kb on - strand at 232.599 kb on - strand at 232.599 kb on - strand at 232.599 kb on - strand at 232.692 kb on + strand, within GFF5355 at 232.692 kb on + strand, within GFF5355 at 232.692 kb on + strand, within GFF5355 at 232.692 kb on + strand, within GFF5355 at 232.693 kb on - strand, within GFF5355 at 232.693 kb on - strand, within GFF5355 at 232.693 kb on - strand, within GFF5355 at 232.693 kb on - strand, within GFF5355 at 232.770 kb on + strand at 232.771 kb on - strand at 232.771 kb on - strand at 232.771 kb on - strand at 232.771 kb on - strand at 232.802 kb on + strand at 232.802 kb on + strand at 232.802 kb on + strand at 232.802 kb on + strand at 232.802 kb on + strand at 232.802 kb on + strand at 232.802 kb on + strand at 232.803 kb on - strand at 232.803 kb on - strand at 232.803 kb on - strand at 232.803 kb on - strand at 232.803 kb on - strand at 232.803 kb on - strand at 232.803 kb on - strand at 232.840 kb on + strand at 232.840 kb on + strand at 232.840 kb on + strand at 232.840 kb on + strand at 232.898 kb on + strand, within GFF5356 at 232.898 kb on + strand, within GFF5356 at 232.898 kb on + strand, within GFF5356 at 232.899 kb on - strand, within GFF5356 at 232.899 kb on - strand, within GFF5356 at 232.899 kb on - strand, within GFF5356 at 232.899 kb on - strand, within GFF5356 at 232.899 kb on - strand, within GFF5356 at 233.116 kb on - strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.158 kb on + strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.159 kb on - strand, within GFF5356 at 233.294 kb on - strand, within GFF5356 at 233.294 kb on - strand, within GFF5356 at 233.294 kb on - strand, within GFF5356 at 234.343 kb on - strand at 234.356 kb on + strand at 234.368 kb on + strand at 234.368 kb on + strand at 234.368 kb on + strand at 234.368 kb on + strand at 234.368 kb on + strand at 234.369 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 231,880 + GFF5354 0.47 +0.6 231,880 + GFF5354 0.47 +0.4 231,880 + GFF5354 0.47 -0.1 231,880 + GFF5354 0.47 -0.6 231,880 + GFF5354 0.47 +0.9 231,881 - GFF5354 0.47 +0.5 231,881 - GFF5354 0.47 -0.2 231,881 - GFF5354 0.47 +0.1 231,881 - GFF5354 0.47 -1.0 231,881 - GFF5354 0.47 +2.0 231,988 + GFF5354 0.55 +0.7 231,989 - GFF5354 0.55 -0.8 232,450 + -0.6 232,450 + -0.2 232,451 - +0.2 232,451 - -0.8 232,451 - -1.1 232,504 + +0.4 232,504 + -0.3 232,504 + -0.8 232,504 + +0.5 232,504 + -0.2 232,504 + -0.9 232,504 + +3.3 232,504 + +1.1 232,505 - +1.7 232,505 - -2.2 232,505 - +0.7 232,505 - -1.4 232,505 - -1.1 232,505 - +1.1 232,575 + +0.1 232,576 - +0.8 232,592 + +0.0 232,592 + -0.9 232,592 + -0.0 232,592 + +0.3 232,592 + -0.0 232,592 + +0.3 232,592 + +0.4 232,592 + +1.7 232,593 - -0.6 232,593 - -0.4 232,593 - +1.7 232,593 - +0.1 232,593 - -0.9 232,598 + +0.5 232,598 + -2.9 232,598 + +0.3 232,598 + -0.2 232,598 + +0.1 232,598 + -0.3 232,598 + -0.6 232,599 - +0.0 232,599 - -0.4 232,599 - +0.7 232,599 - -0.2 232,599 - +0.7 232,599 - -0.6 232,599 - -0.2 232,692 + GFF5355 0.43 -0.4 232,692 + GFF5355 0.43 -1.6 232,692 + GFF5355 0.43 +0.8 232,692 + GFF5355 0.43 -1.8 232,693 - GFF5355 0.43 -0.2 232,693 - GFF5355 0.43 -0.9 232,693 - GFF5355 0.43 +1.7 232,693 - GFF5355 0.43 -0.4 232,770 + +1.7 232,771 - -0.5 232,771 - -0.3 232,771 - +0.4 232,771 - -0.7 232,802 + -0.6 232,802 + -0.1 232,802 + -0.4 232,802 + +0.1 232,802 + +1.2 232,802 + +1.3 232,802 + +0.5 232,803 - +1.7 232,803 - -0.5 232,803 - -0.0 232,803 - +0.0 232,803 - +0.1 232,803 - -0.3 232,803 - -0.1 232,840 + +0.4 232,840 + -0.2 232,840 + -1.0 232,840 + +0.0 232,898 + GFF5356 0.16 -1.7 232,898 + GFF5356 0.16 +0.3 232,898 + GFF5356 0.16 -2.8 232,899 - GFF5356 0.16 -0.3 232,899 - GFF5356 0.16 +0.6 232,899 - GFF5356 0.16 -0.5 232,899 - GFF5356 0.16 -2.5 232,899 - GFF5356 0.16 +0.6 233,116 - GFF5356 0.44 -1.1 233,158 + GFF5356 0.50 -1.1 233,158 + GFF5356 0.50 -0.4 233,158 + GFF5356 0.50 +0.1 233,158 + GFF5356 0.50 -1.0 233,158 + GFF5356 0.50 -0.6 233,158 + GFF5356 0.50 -0.6 233,158 + GFF5356 0.50 -1.3 233,158 + GFF5356 0.50 -1.1 233,158 + GFF5356 0.50 -1.6 233,159 - GFF5356 0.50 -1.0 233,159 - GFF5356 0.50 -0.3 233,159 - GFF5356 0.50 -0.5 233,159 - GFF5356 0.50 +0.5 233,159 - GFF5356 0.50 -0.3 233,159 - GFF5356 0.50 -0.5 233,159 - GFF5356 0.50 -0.6 233,159 - GFF5356 0.50 -0.9 233,159 - GFF5356 0.50 +0.1 233,159 - GFF5356 0.50 -1.9 233,159 - GFF5356 0.50 -0.2 233,159 - GFF5356 0.50 -1.3 233,159 - GFF5356 0.50 -2.1 233,159 - GFF5356 0.50 -1.5 233,159 - GFF5356 0.50 +0.5 233,294 - GFF5356 0.68 +0.9 233,294 - GFF5356 0.68 -1.4 233,294 - GFF5356 0.68 -0.2 234,343 - +0.7 234,356 + -0.5 234,368 + +0.3 234,368 + -0.3 234,368 + -0.3 234,368 + +0.3 234,368 + +0.4 234,369 - -1.1
Or see this region's nucleotide sequence