Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5350 and GFF5351 are separated by 63 nucleotides GFF5351 and GFF5352 are separated by 34 nucleotides GFF5352 and GFF5353 are separated by 21 nucleotides GFF5353 and GFF5354 overlap by 4 nucleotides
GFF5350 - Imidazole glycerol phosphate synthase amidotransferase subunit HisH, at 227,670 to 228,326
GFF5350
GFF5351 - Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16), at 228,390 to 229,130
GFF5351
GFF5352 - Split AAA-ATPase protein PA0787, at 229,165 to 230,337
GFF5352
GFF5353 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 230,359 to 231,267
GFF5353
GFF5354 - C4-dicarboxylate transport protein, at 231,264 to 232,571
GFF5354
Position (kb) 229
230
231 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 229.145 kb on + strand at 229.145 kb on + strand at 229.145 kb on + strand at 229.145 kb on + strand at 229.192 kb on + strand at 229.192 kb on + strand at 229.192 kb on + strand at 229.192 kb on + strand at 229.193 kb on - strand at 229.193 kb on - strand at 229.276 kb on + strand at 229.468 kb on - strand, within GFF5352 at 229.489 kb on + strand, within GFF5352 at 229.489 kb on + strand, within GFF5352 at 229.489 kb on + strand, within GFF5352 at 229.489 kb on + strand, within GFF5352 at 229.490 kb on - strand, within GFF5352 at 229.490 kb on - strand, within GFF5352 at 229.490 kb on - strand, within GFF5352 at 229.490 kb on - strand, within GFF5352 at 229.566 kb on + strand, within GFF5352 at 229.566 kb on + strand, within GFF5352 at 229.567 kb on - strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.769 kb on - strand, within GFF5352 at 229.769 kb on - strand, within GFF5352 at 230.047 kb on + strand, within GFF5352 at 230.047 kb on + strand, within GFF5352 at 230.047 kb on + strand, within GFF5352 at 230.047 kb on + strand, within GFF5352 at 230.048 kb on - strand, within GFF5352 at 230.048 kb on - strand, within GFF5352 at 230.360 kb on + strand at 230.360 kb on + strand at 230.360 kb on + strand at 230.361 kb on - strand at 230.361 kb on - strand at 230.361 kb on - strand at 230.465 kb on + strand, within GFF5353 at 230.465 kb on + strand, within GFF5353 at 230.465 kb on + strand, within GFF5353 at 230.465 kb on + strand, within GFF5353 at 230.465 kb on + strand, within GFF5353 at 230.466 kb on - strand, within GFF5353 at 230.466 kb on - strand, within GFF5353 at 230.466 kb on - strand, within GFF5353 at 230.549 kb on + strand, within GFF5353 at 230.549 kb on + strand, within GFF5353 at 230.550 kb on - strand, within GFF5353 at 230.550 kb on - strand, within GFF5353 at 230.550 kb on - strand, within GFF5353 at 230.550 kb on - strand, within GFF5353 at 230.550 kb on - strand, within GFF5353 at 230.570 kb on + strand, within GFF5353 at 230.570 kb on + strand, within GFF5353 at 230.570 kb on + strand, within GFF5353 at 230.570 kb on + strand, within GFF5353 at 230.570 kb on + strand, within GFF5353 at 230.570 kb on + strand, within GFF5353 at 230.571 kb on - strand, within GFF5353 at 230.571 kb on - strand, within GFF5353 at 230.571 kb on - strand, within GFF5353 at 230.571 kb on - strand, within GFF5353 at 230.634 kb on - strand, within GFF5353 at 230.634 kb on - strand, within GFF5353 at 231.065 kb on + strand, within GFF5353 at 231.066 kb on - strand, within GFF5353
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 229,145 + +0.3 229,145 + +1.2 229,145 + -0.5 229,145 + -0.0 229,192 + -0.7 229,192 + -0.6 229,192 + -1.1 229,192 + -0.5 229,193 - -0.3 229,193 - -0.1 229,276 + +0.3 229,468 - GFF5352 0.26 +0.1 229,489 + GFF5352 0.28 -0.9 229,489 + GFF5352 0.28 -0.4 229,489 + GFF5352 0.28 -0.7 229,489 + GFF5352 0.28 +1.4 229,490 - GFF5352 0.28 +0.8 229,490 - GFF5352 0.28 -0.9 229,490 - GFF5352 0.28 -0.8 229,490 - GFF5352 0.28 -0.9 229,566 + GFF5352 0.34 +0.2 229,566 + GFF5352 0.34 +0.1 229,567 - GFF5352 0.34 +0.7 229,768 + GFF5352 0.51 +0.3 229,768 + GFF5352 0.51 +0.1 229,768 + GFF5352 0.51 +0.6 229,768 + GFF5352 0.51 -0.4 229,768 + GFF5352 0.51 +3.7 229,768 + GFF5352 0.51 -0.7 229,768 + GFF5352 0.51 -0.5 229,768 + GFF5352 0.51 +0.1 229,768 + GFF5352 0.51 -0.6 229,768 + GFF5352 0.51 -0.6 229,769 - GFF5352 0.51 +2.1 229,769 - GFF5352 0.51 +0.5 230,047 + GFF5352 0.75 +0.2 230,047 + GFF5352 0.75 +0.8 230,047 + GFF5352 0.75 +0.2 230,047 + GFF5352 0.75 -1.0 230,048 - GFF5352 0.75 +1.0 230,048 - GFF5352 0.75 +0.2 230,360 + +1.3 230,360 + -1.7 230,360 + +0.1 230,361 - +0.2 230,361 - +0.6 230,361 - -0.3 230,465 + GFF5353 0.12 +0.2 230,465 + GFF5353 0.12 +0.1 230,465 + GFF5353 0.12 +0.0 230,465 + GFF5353 0.12 +1.0 230,465 + GFF5353 0.12 -0.8 230,466 - GFF5353 0.12 -2.0 230,466 - GFF5353 0.12 -1.1 230,466 - GFF5353 0.12 -0.9 230,549 + GFF5353 0.21 -0.2 230,549 + GFF5353 0.21 -0.5 230,550 - GFF5353 0.21 -0.0 230,550 - GFF5353 0.21 +0.3 230,550 - GFF5353 0.21 +0.0 230,550 - GFF5353 0.21 +0.6 230,550 - GFF5353 0.21 +0.7 230,570 + GFF5353 0.23 +0.9 230,570 + GFF5353 0.23 +0.5 230,570 + GFF5353 0.23 -0.3 230,570 + GFF5353 0.23 +0.7 230,570 + GFF5353 0.23 -0.2 230,570 + GFF5353 0.23 -0.4 230,571 - GFF5353 0.23 -0.6 230,571 - GFF5353 0.23 -0.6 230,571 - GFF5353 0.23 -0.5 230,571 - GFF5353 0.23 -0.3 230,634 - GFF5353 0.30 -0.4 230,634 - GFF5353 0.30 +0.0 231,065 + GFF5353 0.78 +0.7 231,066 - GFF5353 0.78 -0.5
Or see this region's nucleotide sequence