Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5257 and GFF5258 are separated by 94 nucleotides GFF5258 and GFF5259 overlap by 1 nucleotides GFF5259 and GFF5260 are separated by 73 nucleotides
GFF5257 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 122,543 to 123,580
GFF5257
GFF5258 - hypothetical protein, at 123,675 to 123,854
GFF5258
GFF5259 - Na+/solute symporter, at 123,854 to 125,359
GFF5259
GFF5260 - Uncharacterized protein ygiD, at 125,433 to 126,341
GFF5260
Position (kb) 123
124
125
126 Strain fitness (log2 ratio) -2
-1
0
1
2 at 122.909 kb on + strand, within GFF5257 at 122.910 kb on - strand, within GFF5257 at 123.059 kb on + strand, within GFF5257 at 123.059 kb on + strand, within GFF5257 at 123.059 kb on + strand, within GFF5257 at 123.060 kb on - strand, within GFF5257 at 123.060 kb on - strand, within GFF5257 at 123.060 kb on - strand, within GFF5257 at 123.060 kb on - strand, within GFF5257 at 123.230 kb on + strand, within GFF5257 at 123.230 kb on + strand, within GFF5257 at 123.230 kb on + strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.356 kb on + strand, within GFF5257 at 123.357 kb on - strand, within GFF5257 at 123.357 kb on - strand, within GFF5257 at 123.578 kb on + strand at 123.592 kb on - strand at 123.627 kb on + strand at 123.627 kb on + strand at 123.627 kb on + strand at 123.627 kb on + strand at 123.628 kb on - strand at 123.628 kb on - strand at 123.629 kb on + strand at 123.629 kb on + strand at 123.629 kb on + strand at 123.629 kb on + strand at 123.630 kb on - strand at 123.630 kb on - strand at 123.630 kb on - strand at 123.726 kb on + strand, within GFF5258 at 123.727 kb on - strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.769 kb on - strand, within GFF5258 at 123.769 kb on - strand, within GFF5258 at 123.769 kb on - strand, within GFF5258 at 123.774 kb on + strand, within GFF5258 at 123.774 kb on + strand, within GFF5258 at 123.774 kb on + strand, within GFF5258 at 123.774 kb on + strand, within GFF5258 at 123.775 kb on - strand, within GFF5258 at 123.775 kb on - strand, within GFF5258 at 123.775 kb on - strand, within GFF5258 at 123.823 kb on - strand, within GFF5258 at 123.834 kb on + strand, within GFF5258 at 123.835 kb on - strand, within GFF5258 at 124.049 kb on + strand, within GFF5259 at 124.050 kb on - strand, within GFF5259 at 124.050 kb on - strand, within GFF5259 at 124.050 kb on - strand, within GFF5259 at 124.058 kb on + strand, within GFF5259 at 124.058 kb on + strand, within GFF5259 at 124.059 kb on - strand, within GFF5259 at 124.059 kb on - strand, within GFF5259 at 124.059 kb on - strand, within GFF5259 at 124.059 kb on - strand, within GFF5259 at 124.088 kb on + strand, within GFF5259 at 124.089 kb on - strand, within GFF5259 at 124.103 kb on + strand, within GFF5259 at 124.103 kb on + strand, within GFF5259 at 124.103 kb on + strand, within GFF5259 at 124.124 kb on + strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.220 kb on + strand, within GFF5259 at 124.221 kb on - strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.388 kb on + strand, within GFF5259 at 124.388 kb on + strand, within GFF5259 at 124.388 kb on + strand, within GFF5259 at 124.389 kb on - strand, within GFF5259 at 124.389 kb on - strand, within GFF5259 at 124.394 kb on + strand, within GFF5259 at 124.394 kb on + strand, within GFF5259 at 124.394 kb on + strand, within GFF5259 at 124.395 kb on - strand, within GFF5259 at 124.395 kb on - strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.583 kb on + strand, within GFF5259 at 124.583 kb on + strand, within GFF5259 at 124.583 kb on + strand, within GFF5259 at 124.584 kb on - strand, within GFF5259 at 124.584 kb on - strand, within GFF5259 at 124.584 kb on - strand, within GFF5259 at 124.584 kb on - strand, within GFF5259 at 124.592 kb on + strand, within GFF5259 at 124.592 kb on + strand, within GFF5259 at 124.593 kb on - strand, within GFF5259 at 124.593 kb on - strand, within GFF5259 at 124.593 kb on - strand, within GFF5259 at 124.593 kb on - strand, within GFF5259 at 124.634 kb on + strand, within GFF5259 at 124.634 kb on + strand, within GFF5259 at 124.634 kb on + strand, within GFF5259 at 124.635 kb on - strand, within GFF5259 at 124.635 kb on - strand, within GFF5259 at 124.635 kb on - strand, within GFF5259 at 124.712 kb on + strand, within GFF5259 at 124.712 kb on + strand, within GFF5259 at 124.712 kb on + strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 125.179 kb on + strand, within GFF5259 at 125.179 kb on + strand, within GFF5259 at 125.252 kb on + strand at 125.358 kb on - strand at 125.417 kb on - strand at 125.417 kb on - strand at 125.794 kb on - strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.911 kb on - strand, within GFF5260 at 125.959 kb on - strand, within GFF5260 at 125.982 kb on + strand, within GFF5260 at 125.983 kb on - strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.229 kb on - strand, within GFF5260 at 126.229 kb on - strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 122,909 + GFF5257 0.35 -0.1 122,910 - GFF5257 0.35 -0.5 123,059 + GFF5257 0.50 -0.1 123,059 + GFF5257 0.50 -1.2 123,059 + GFF5257 0.50 +0.8 123,060 - GFF5257 0.50 -0.3 123,060 - GFF5257 0.50 -0.4 123,060 - GFF5257 0.50 -0.3 123,060 - GFF5257 0.50 +2.5 123,230 + GFF5257 0.66 -0.0 123,230 + GFF5257 0.66 -0.6 123,230 + GFF5257 0.66 +0.1 123,231 - GFF5257 0.66 -1.0 123,231 - GFF5257 0.66 +0.3 123,231 - GFF5257 0.66 -0.0 123,231 - GFF5257 0.66 -0.1 123,231 - GFF5257 0.66 -0.2 123,231 - GFF5257 0.66 -0.7 123,293 + GFF5257 0.72 +0.3 123,293 + GFF5257 0.72 -0.7 123,293 + GFF5257 0.72 -1.9 123,293 + GFF5257 0.72 +0.1 123,293 + GFF5257 0.72 -0.4 123,293 + GFF5257 0.72 +0.7 123,294 - GFF5257 0.72 +2.5 123,294 - GFF5257 0.72 -0.5 123,294 - GFF5257 0.72 -0.4 123,294 - GFF5257 0.72 -2.1 123,294 - GFF5257 0.72 -1.0 123,294 - GFF5257 0.72 -0.6 123,294 - GFF5257 0.72 +1.7 123,356 + GFF5257 0.78 +1.0 123,357 - GFF5257 0.78 +1.2 123,357 - GFF5257 0.78 -1.2 123,578 + +0.6 123,592 - +0.4 123,627 + -1.1 123,627 + -1.3 123,627 + +0.5 123,627 + +0.1 123,628 - -0.2 123,628 - +1.3 123,629 + -0.2 123,629 + -1.2 123,629 + +0.0 123,629 + -1.3 123,630 - +0.5 123,630 - -0.2 123,630 - -0.5 123,726 + GFF5258 0.28 +0.4 123,727 - GFF5258 0.29 +0.5 123,768 + GFF5258 0.52 -0.6 123,768 + GFF5258 0.52 -0.1 123,768 + GFF5258 0.52 +0.2 123,768 + GFF5258 0.52 +0.2 123,768 + GFF5258 0.52 -0.1 123,769 - GFF5258 0.52 -1.6 123,769 - GFF5258 0.52 +1.1 123,769 - GFF5258 0.52 -0.2 123,774 + GFF5258 0.55 -0.9 123,774 + GFF5258 0.55 +0.0 123,774 + GFF5258 0.55 +0.2 123,774 + GFF5258 0.55 -0.0 123,775 - GFF5258 0.56 -0.8 123,775 - GFF5258 0.56 -1.2 123,775 - GFF5258 0.56 +0.5 123,823 - GFF5258 0.82 +0.5 123,834 + GFF5258 0.88 -0.4 123,835 - GFF5258 0.89 -0.3 124,049 + GFF5259 0.13 -0.4 124,050 - GFF5259 0.13 +0.2 124,050 - GFF5259 0.13 +0.7 124,050 - GFF5259 0.13 +0.6 124,058 + GFF5259 0.14 -0.3 124,058 + GFF5259 0.14 +0.2 124,059 - GFF5259 0.14 -0.4 124,059 - GFF5259 0.14 +1.3 124,059 - GFF5259 0.14 +0.2 124,059 - GFF5259 0.14 -0.2 124,088 + GFF5259 0.16 -0.3 124,089 - GFF5259 0.16 -0.0 124,103 + GFF5259 0.17 -0.0 124,103 + GFF5259 0.17 +0.1 124,103 + GFF5259 0.17 -0.0 124,124 + GFF5259 0.18 -0.5 124,125 - GFF5259 0.18 -0.1 124,125 - GFF5259 0.18 +0.0 124,125 - GFF5259 0.18 -0.6 124,125 - GFF5259 0.18 -0.5 124,125 - GFF5259 0.18 -0.1 124,125 - GFF5259 0.18 +0.3 124,220 + GFF5259 0.24 +1.0 124,221 - GFF5259 0.24 +1.2 124,289 + GFF5259 0.29 -0.2 124,289 + GFF5259 0.29 -0.3 124,289 + GFF5259 0.29 +0.1 124,289 + GFF5259 0.29 +1.0 124,289 + GFF5259 0.29 +0.3 124,289 + GFF5259 0.29 -0.2 124,290 - GFF5259 0.29 +0.5 124,290 - GFF5259 0.29 -0.3 124,290 - GFF5259 0.29 -0.4 124,290 - GFF5259 0.29 +0.0 124,290 - GFF5259 0.29 -1.2 124,290 - GFF5259 0.29 -0.1 124,290 - GFF5259 0.29 +0.9 124,290 - GFF5259 0.29 +0.3 124,388 + GFF5259 0.35 -0.9 124,388 + GFF5259 0.35 +0.4 124,388 + GFF5259 0.35 +0.9 124,389 - GFF5259 0.36 -0.9 124,389 - GFF5259 0.36 +1.1 124,394 + GFF5259 0.36 -0.4 124,394 + GFF5259 0.36 +0.4 124,394 + GFF5259 0.36 +1.0 124,395 - GFF5259 0.36 -0.1 124,395 - GFF5259 0.36 -1.1 124,451 + GFF5259 0.40 -0.6 124,451 + GFF5259 0.40 +2.2 124,451 + GFF5259 0.40 -1.6 124,451 + GFF5259 0.40 +0.5 124,451 + GFF5259 0.40 +0.5 124,452 - GFF5259 0.40 +0.1 124,452 - GFF5259 0.40 -0.2 124,452 - GFF5259 0.40 -0.2 124,452 - GFF5259 0.40 -0.6 124,452 - GFF5259 0.40 -0.9 124,452 - GFF5259 0.40 -0.1 124,452 - GFF5259 0.40 -0.4 124,452 - GFF5259 0.40 -0.5 124,452 - GFF5259 0.40 -1.6 124,452 - GFF5259 0.40 -1.6 124,452 - GFF5259 0.40 -0.1 124,452 - GFF5259 0.40 -0.1 124,452 - GFF5259 0.40 +0.1 124,583 + GFF5259 0.48 -0.5 124,583 + GFF5259 0.48 +1.2 124,583 + GFF5259 0.48 -0.1 124,584 - GFF5259 0.48 -1.5 124,584 - GFF5259 0.48 -1.5 124,584 - GFF5259 0.48 +0.4 124,584 - GFF5259 0.48 -2.2 124,592 + GFF5259 0.49 -0.6 124,592 + GFF5259 0.49 +0.0 124,593 - GFF5259 0.49 -0.4 124,593 - GFF5259 0.49 +0.8 124,593 - GFF5259 0.49 -0.3 124,593 - GFF5259 0.49 +2.0 124,634 + GFF5259 0.52 +0.8 124,634 + GFF5259 0.52 +0.4 124,634 + GFF5259 0.52 +1.1 124,635 - GFF5259 0.52 +0.3 124,635 - GFF5259 0.52 -0.5 124,635 - GFF5259 0.52 +1.3 124,712 + GFF5259 0.57 -1.2 124,712 + GFF5259 0.57 +0.0 124,712 + GFF5259 0.57 +0.4 124,713 - GFF5259 0.57 -0.0 124,713 - GFF5259 0.57 -0.3 124,713 - GFF5259 0.57 -0.2 124,713 - GFF5259 0.57 -0.1 124,713 - GFF5259 0.57 -1.1 124,748 + GFF5259 0.59 +0.1 124,748 + GFF5259 0.59 +2.2 124,748 + GFF5259 0.59 +0.3 124,748 + GFF5259 0.59 -2.4 124,748 + GFF5259 0.59 -1.4 124,748 + GFF5259 0.59 +0.0 124,748 + GFF5259 0.59 +0.7 124,748 + GFF5259 0.59 -0.5 124,937 + GFF5259 0.72 -0.2 124,937 + GFF5259 0.72 +2.0 124,937 + GFF5259 0.72 +0.6 124,937 + GFF5259 0.72 -0.4 124,937 + GFF5259 0.72 +0.3 124,937 + GFF5259 0.72 +0.1 124,937 + GFF5259 0.72 +0.1 124,937 + GFF5259 0.72 -0.3 124,938 - GFF5259 0.72 +0.3 124,938 - GFF5259 0.72 -1.3 124,938 - GFF5259 0.72 +0.3 124,938 - GFF5259 0.72 -0.3 124,938 - GFF5259 0.72 +1.1 124,938 - GFF5259 0.72 +0.3 124,938 - GFF5259 0.72 +0.1 124,938 - GFF5259 0.72 -1.0 124,938 - GFF5259 0.72 -1.3 124,938 - GFF5259 0.72 -0.9 124,938 - GFF5259 0.72 -1.5 124,938 - GFF5259 0.72 +0.7 124,938 - GFF5259 0.72 +0.1 124,938 - GFF5259 0.72 -0.3 124,938 - GFF5259 0.72 -0.4 125,179 + GFF5259 0.88 -2.3 125,179 + GFF5259 0.88 +1.0 125,252 + -0.6 125,358 - +0.1 125,417 - +1.7 125,417 - -0.4 125,794 - GFF5260 0.40 -0.4 125,910 + GFF5260 0.52 +0.0 125,910 + GFF5260 0.52 -0.2 125,910 + GFF5260 0.52 +0.3 125,910 + GFF5260 0.52 +0.2 125,910 + GFF5260 0.52 -0.3 125,910 + GFF5260 0.52 +0.7 125,910 + GFF5260 0.52 -0.8 125,910 + GFF5260 0.52 -0.1 125,911 - GFF5260 0.53 -0.2 125,959 - GFF5260 0.58 +0.3 125,982 + GFF5260 0.60 -0.5 125,983 - GFF5260 0.61 -0.7 126,228 + GFF5260 0.87 +0.1 126,228 + GFF5260 0.87 +0.1 126,228 + GFF5260 0.87 +1.2 126,228 + GFF5260 0.87 -0.1 126,228 + GFF5260 0.87 -0.1 126,229 - GFF5260 0.88 +0.5 126,229 - GFF5260 0.88 +0.2 126,231 + GFF5260 0.88 +0.8 126,231 + GFF5260 0.88 +0.1 126,231 + GFF5260 0.88 +0.3 126,231 + GFF5260 0.88 -0.9 126,231 + GFF5260 0.88 +0.3 126,231 + GFF5260 0.88 -0.3 126,232 - GFF5260 0.88 +0.1 126,232 - GFF5260 0.88 +0.4 126,232 - GFF5260 0.88 -1.5 126,288 + +0.2 126,288 + -0.8 126,288 + -0.7 126,288 + -0.1 126,288 + -0.0 126,288 + +0.8 126,289 - +0.6 126,289 - +0.6 126,289 - +0.4 126,289 - -0.3 126,289 - +0.5 126,289 - +0.1 126,352 - +0.3
Or see this region's nucleotide sequence