Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5106 and GFF5107 are separated by 94 nucleotides GFF5107 and GFF5108 overlap by 47 nucleotides GFF5108 and GFF5109 overlap by 4 nucleotides
GFF5106 - Permease of the drug/metabolite transporter (DMT) superfamily, at 207,579 to 208,523
GFF5106
GFF5107 - Transcriptional regulator, LysR family, at 208,618 to 209,568
GFF5107
GFF5108 - FIG00348319: hypothetical protein, at 209,522 to 210,280
GFF5108
GFF5109 - Alanine racemase (EC 5.1.1.1), at 210,277 to 211,380
GFF5109
Position (kb)
208
209
210 Strain fitness (log2 ratio)
-1
0
1 at 207.679 kb on + strand, within GFF5106 at 207.679 kb on + strand, within GFF5106 at 207.679 kb on + strand, within GFF5106 at 207.680 kb on - strand, within GFF5106 at 207.680 kb on - strand, within GFF5106 at 207.805 kb on + strand, within GFF5106 at 207.805 kb on + strand, within GFF5106 at 207.806 kb on - strand, within GFF5106 at 207.806 kb on - strand, within GFF5106 at 207.806 kb on - strand, within GFF5106 at 207.806 kb on - strand, within GFF5106 at 207.806 kb on - strand, within GFF5106 at 208.402 kb on + strand, within GFF5106 at 208.402 kb on + strand, within GFF5106 at 208.402 kb on + strand, within GFF5106 at 208.403 kb on - strand, within GFF5106 at 208.403 kb on - strand, within GFF5106 at 208.403 kb on - strand, within GFF5106 at 208.523 kb on + strand at 208.524 kb on - strand at 208.524 kb on - strand at 208.524 kb on - strand at 208.524 kb on - strand at 208.524 kb on - strand at 208.991 kb on - strand, within GFF5107 at 209.150 kb on - strand, within GFF5107 at 209.150 kb on - strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.290 kb on + strand, within GFF5107 at 209.291 kb on - strand, within GFF5107 at 209.291 kb on - strand, within GFF5107 at 209.291 kb on - strand, within GFF5107 at 209.291 kb on - strand, within GFF5107 at 209.291 kb on - strand, within GFF5107 at 209.291 kb on - strand, within GFF5107 at 209.405 kb on + strand, within GFF5107 at 209.434 kb on + strand, within GFF5107 at 209.434 kb on + strand, within GFF5107 at 209.434 kb on + strand, within GFF5107 at 209.435 kb on - strand, within GFF5107 at 209.435 kb on - strand, within GFF5107 at 209.435 kb on - strand, within GFF5107 at 209.435 kb on - strand, within GFF5107 at 209.459 kb on - strand, within GFF5107 at 209.459 kb on - strand, within GFF5107 at 209.709 kb on + strand, within GFF5108 at 209.709 kb on + strand, within GFF5108 at 209.709 kb on + strand, within GFF5108 at 209.709 kb on + strand, within GFF5108 at 209.709 kb on + strand, within GFF5108 at 209.709 kb on + strand, within GFF5108 at 209.709 kb on + strand, within GFF5108 at 209.709 kb on + strand, within GFF5108 at 209.710 kb on - strand, within GFF5108 at 209.710 kb on - strand, within GFF5108 at 209.710 kb on - strand, within GFF5108 at 209.710 kb on - strand, within GFF5108 at 209.710 kb on - strand, within GFF5108 at 209.710 kb on - strand, within GFF5108 at 209.710 kb on - strand, within GFF5108 at 209.760 kb on + strand, within GFF5108 at 209.761 kb on - strand, within GFF5108 at 209.920 kb on + strand, within GFF5108 at 209.920 kb on + strand, within GFF5108 at 209.920 kb on + strand, within GFF5108 at 210.003 kb on + strand, within GFF5108 at 210.063 kb on + strand, within GFF5108 at 210.207 kb on + strand at 210.207 kb on + strand at 210.208 kb on - strand at 210.208 kb on - strand at 210.208 kb on - strand at 210.208 kb on - strand at 210.208 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 207,679 + GFF5106 0.11 +1.4 207,679 + GFF5106 0.11 -0.9 207,679 + GFF5106 0.11 -0.0 207,680 - GFF5106 0.11 -0.4 207,680 - GFF5106 0.11 +0.2 207,805 + GFF5106 0.24 +0.7 207,805 + GFF5106 0.24 -0.1 207,806 - GFF5106 0.24 +0.5 207,806 - GFF5106 0.24 +1.3 207,806 - GFF5106 0.24 +0.2 207,806 - GFF5106 0.24 +0.3 207,806 - GFF5106 0.24 -0.3 208,402 + GFF5106 0.87 -0.4 208,402 + GFF5106 0.87 -0.2 208,402 + GFF5106 0.87 -0.3 208,403 - GFF5106 0.87 -1.3 208,403 - GFF5106 0.87 -0.6 208,403 - GFF5106 0.87 -1.2 208,523 + +0.9 208,524 - +0.2 208,524 - +0.6 208,524 - -0.1 208,524 - -0.3 208,524 - -0.4 208,991 - GFF5107 0.39 +0.6 209,150 - GFF5107 0.56 -0.4 209,150 - GFF5107 0.56 +0.6 209,290 + GFF5107 0.71 +1.3 209,290 + GFF5107 0.71 -0.9 209,290 + GFF5107 0.71 -0.1 209,290 + GFF5107 0.71 -1.0 209,290 + GFF5107 0.71 -0.4 209,290 + GFF5107 0.71 +0.7 209,290 + GFF5107 0.71 +0.7 209,290 + GFF5107 0.71 -1.5 209,290 + GFF5107 0.71 -0.7 209,290 + GFF5107 0.71 -0.3 209,290 + GFF5107 0.71 +0.7 209,290 + GFF5107 0.71 +0.3 209,290 + GFF5107 0.71 +0.3 209,291 - GFF5107 0.71 -0.3 209,291 - GFF5107 0.71 +0.3 209,291 - GFF5107 0.71 +0.7 209,291 - GFF5107 0.71 +1.7 209,291 - GFF5107 0.71 +0.4 209,291 - GFF5107 0.71 -0.1 209,405 + GFF5107 0.83 +0.3 209,434 + GFF5107 0.86 +1.3 209,434 + GFF5107 0.86 -0.9 209,434 + GFF5107 0.86 +0.3 209,435 - GFF5107 0.86 -1.0 209,435 - GFF5107 0.86 -0.4 209,435 - GFF5107 0.86 +0.3 209,435 - GFF5107 0.86 -0.3 209,459 - GFF5107 0.88 -1.1 209,459 - GFF5107 0.88 +0.3 209,709 + GFF5108 0.25 +0.7 209,709 + GFF5108 0.25 -0.2 209,709 + GFF5108 0.25 -0.1 209,709 + GFF5108 0.25 -0.2 209,709 + GFF5108 0.25 +0.2 209,709 + GFF5108 0.25 -0.3 209,709 + GFF5108 0.25 -0.3 209,709 + GFF5108 0.25 -1.0 209,710 - GFF5108 0.25 -1.0 209,710 - GFF5108 0.25 -0.8 209,710 - GFF5108 0.25 -0.4 209,710 - GFF5108 0.25 -0.4 209,710 - GFF5108 0.25 -0.1 209,710 - GFF5108 0.25 +0.7 209,710 - GFF5108 0.25 -0.4 209,760 + GFF5108 0.31 -0.1 209,761 - GFF5108 0.31 -0.1 209,920 + GFF5108 0.52 +1.2 209,920 + GFF5108 0.52 -0.3 209,920 + GFF5108 0.52 -0.5 210,003 + GFF5108 0.63 -0.2 210,063 + GFF5108 0.71 -0.6 210,207 + -0.4 210,207 + +0.4 210,208 - +0.1 210,208 - -0.3 210,208 - -0.7 210,208 - +1.1 210,208 - -0.5
Or see this region's nucleotide sequence