Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4542 and GFF4543 overlap by 4 nucleotides GFF4543 and GFF4544 overlap by 4 nucleotides GFF4544 and GFF4545 are separated by 161 nucleotides
GFF4542 - hypothetical protein, at 180,870 to 181,364
GFF4542
GFF4543 - no description, at 181,361 to 181,795
GFF4543
GFF4544 - RNA polymerase sigma factor RpoE, at 181,792 to 182,292
GFF4544
GFF4545 - Transcriptional regulator, AraC family, at 182,454 to 183,251
GFF4545
Position (kb)
181
182
183 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 180.871 kb on + strand at 180.871 kb on + strand at 180.872 kb on - strand at 180.872 kb on - strand at 181.384 kb on - strand at 181.758 kb on + strand at 181.758 kb on + strand at 181.759 kb on - strand at 181.759 kb on - strand at 181.759 kb on - strand at 181.803 kb on - strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.811 kb on - strand at 181.811 kb on - strand at 181.811 kb on - strand at 181.811 kb on - strand at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.926 kb on - strand, within GFF4544 at 181.926 kb on - strand, within GFF4544 at 181.926 kb on - strand, within GFF4544 at 181.926 kb on - strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 182.102 kb on + strand, within GFF4544 at 182.103 kb on - strand, within GFF4544 at 182.103 kb on - strand, within GFF4544 at 182.103 kb on - strand, within GFF4544 at 182.327 kb on - strand at 182.408 kb on + strand at 182.408 kb on + strand at 182.408 kb on + strand at 182.485 kb on + strand at 182.548 kb on + strand, within GFF4545 at 182.548 kb on + strand, within GFF4545 at 182.549 kb on - strand, within GFF4545 at 182.549 kb on - strand, within GFF4545 at 182.797 kb on + strand, within GFF4545 at 182.797 kb on + strand, within GFF4545 at 182.797 kb on + strand, within GFF4545 at 182.798 kb on - strand, within GFF4545 at 182.983 kb on + strand, within GFF4545 at 182.983 kb on + strand, within GFF4545 at 182.983 kb on + strand, within GFF4545 at 182.983 kb on + strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.984 kb on - strand, within GFF4545 at 182.995 kb on + strand, within GFF4545 at 182.996 kb on - strand, within GFF4545 at 182.996 kb on - strand, within GFF4545 at 183.055 kb on + strand, within GFF4545 at 183.055 kb on + strand, within GFF4545 at 183.056 kb on - strand, within GFF4545 at 183.056 kb on - strand, within GFF4545 at 183.118 kb on + strand, within GFF4545 at 183.118 kb on + strand, within GFF4545 at 183.118 kb on + strand, within GFF4545 at 183.118 kb on + strand, within GFF4545 at 183.118 kb on + strand, within GFF4545 at 183.118 kb on + strand, within GFF4545 at 183.119 kb on - strand, within GFF4545 at 183.119 kb on - strand, within GFF4545 at 183.119 kb on - strand, within GFF4545 at 183.119 kb on - strand, within GFF4545 at 183.190 kb on + strand at 183.190 kb on + strand at 183.190 kb on + strand at 183.190 kb on + strand at 183.191 kb on - strand at 183.191 kb on - strand at 183.191 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 180,871 + -0.7 180,871 + -0.2 180,872 - -0.4 180,872 - -0.4 181,384 - -1.0 181,758 + -0.6 181,758 + -1.1 181,759 - +0.2 181,759 - -0.7 181,759 - -0.5 181,803 - -0.3 181,810 + +0.6 181,810 + +1.1 181,810 + -2.8 181,810 + -0.6 181,810 + -0.4 181,810 + -1.0 181,811 - -0.6 181,811 - -0.3 181,811 - -1.1 181,811 - -0.9 181,925 + GFF4544 0.27 +0.0 181,925 + GFF4544 0.27 -0.3 181,925 + GFF4544 0.27 -1.6 181,925 + GFF4544 0.27 -0.9 181,925 + GFF4544 0.27 -0.4 181,925 + GFF4544 0.27 +0.3 181,926 - GFF4544 0.27 -0.2 181,926 - GFF4544 0.27 -0.7 181,926 - GFF4544 0.27 -0.0 181,926 - GFF4544 0.27 +0.3 181,928 + GFF4544 0.27 +1.7 181,928 + GFF4544 0.27 -0.6 181,928 + GFF4544 0.27 +0.9 181,928 + GFF4544 0.27 -0.4 181,928 + GFF4544 0.27 -0.6 181,928 + GFF4544 0.27 -0.2 181,928 + GFF4544 0.27 -1.0 181,929 - GFF4544 0.27 -1.7 181,929 - GFF4544 0.27 -0.8 181,929 - GFF4544 0.27 -0.6 181,929 - GFF4544 0.27 +0.3 181,929 - GFF4544 0.27 -0.4 181,929 - GFF4544 0.27 -0.7 182,102 + GFF4544 0.62 +0.3 182,103 - GFF4544 0.62 +0.0 182,103 - GFF4544 0.62 +2.0 182,103 - GFF4544 0.62 -0.9 182,327 - +0.1 182,408 + -0.6 182,408 + -0.5 182,408 + -0.0 182,485 + +0.2 182,548 + GFF4545 0.12 -1.6 182,548 + GFF4545 0.12 -0.5 182,549 - GFF4545 0.12 -0.8 182,549 - GFF4545 0.12 -1.8 182,797 + GFF4545 0.43 -0.8 182,797 + GFF4545 0.43 -0.7 182,797 + GFF4545 0.43 -0.3 182,798 - GFF4545 0.43 -0.9 182,983 + GFF4545 0.66 -1.5 182,983 + GFF4545 0.66 +0.1 182,983 + GFF4545 0.66 +1.2 182,983 + GFF4545 0.66 -1.0 182,984 - GFF4545 0.66 -1.1 182,984 - GFF4545 0.66 -0.7 182,984 - GFF4545 0.66 +0.1 182,984 - GFF4545 0.66 +0.5 182,984 - GFF4545 0.66 -1.0 182,984 - GFF4545 0.66 -0.4 182,984 - GFF4545 0.66 +1.7 182,984 - GFF4545 0.66 -0.1 182,984 - GFF4545 0.66 -0.3 182,995 + GFF4545 0.68 -0.6 182,996 - GFF4545 0.68 -1.9 182,996 - GFF4545 0.68 -0.0 183,055 + GFF4545 0.75 +0.6 183,055 + GFF4545 0.75 +1.1 183,056 - GFF4545 0.75 +0.0 183,056 - GFF4545 0.75 -1.6 183,118 + GFF4545 0.83 -0.6 183,118 + GFF4545 0.83 +0.3 183,118 + GFF4545 0.83 +0.6 183,118 + GFF4545 0.83 -0.7 183,118 + GFF4545 0.83 -0.8 183,118 + GFF4545 0.83 +1.0 183,119 - GFF4545 0.83 +1.1 183,119 - GFF4545 0.83 -0.6 183,119 - GFF4545 0.83 -0.0 183,119 - GFF4545 0.83 -0.8 183,190 + -0.8 183,190 + -0.3 183,190 + -0.8 183,190 + -0.3 183,191 - -0.4 183,191 - -1.4 183,191 - -0.7
Or see this region's nucleotide sequence