Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4412 and GFF4413 overlap by 4 nucleotides GFF4413 and GFF4414 overlap by 4 nucleotides GFF4414 and GFF4415 overlap by 4 nucleotides GFF4415 and GFF4416 overlap by 4 nucleotides GFF4416 and GFF4417 are separated by 12 nucleotides
GFF4412 - Coupling protein VirD4, ATPase required for T-DNA transfer, at 48,447 to 50,480
GFF4412
GFF4413 - CopG domain-containing protein, at 50,477 to 50,953
GFF4413
GFF4414 - Conjugative transfer protein TrbB, at 50,950 to 52,014
GFF4414
GFF4415 - Conjugative transfer protein TrbC, at 52,011 to 52,400
GFF4415
GFF4416 - Conjugative transfer protein TrbD, at 52,397 to 52,669
GFF4416
GFF4417 - Conjugative transfer protein TrbE, at 52,682 to 55,156
GFF4417
Position (kb)
50
51
52
53 Strain fitness (log2 ratio)
-2
-1
0
1 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.026 kb on + strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.027 kb on - strand, within GFF4412 at 50.091 kb on + strand, within GFF4412 at 50.091 kb on + strand, within GFF4412 at 50.091 kb on + strand, within GFF4412 at 50.092 kb on - strand, within GFF4412 at 50.092 kb on - strand, within GFF4412 at 50.092 kb on - strand, within GFF4412 at 50.092 kb on - strand, within GFF4412 at 50.094 kb on + strand, within GFF4412 at 50.094 kb on + strand, within GFF4412 at 50.094 kb on + strand, within GFF4412 at 50.094 kb on + strand, within GFF4412 at 50.166 kb on + strand, within GFF4412 at 50.166 kb on + strand, within GFF4412 at 50.167 kb on - strand, within GFF4412 at 50.167 kb on - strand, within GFF4412 at 50.311 kb on + strand at 50.311 kb on + strand at 50.311 kb on + strand at 50.311 kb on + strand at 50.312 kb on - strand at 50.312 kb on - strand at 50.312 kb on - strand at 50.312 kb on - strand at 50.328 kb on + strand at 50.328 kb on + strand at 50.328 kb on + strand at 50.328 kb on + strand at 50.328 kb on + strand at 50.328 kb on + strand at 50.329 kb on - strand at 50.329 kb on - strand at 50.329 kb on - strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.476 kb on + strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.477 kb on - strand at 50.498 kb on + strand at 50.499 kb on - strand at 50.515 kb on + strand at 50.515 kb on + strand at 50.686 kb on + strand, within GFF4413 at 50.686 kb on + strand, within GFF4413 at 50.686 kb on + strand, within GFF4413 at 50.687 kb on - strand, within GFF4413 at 50.687 kb on - strand, within GFF4413 at 50.687 kb on - strand, within GFF4413 at 50.687 kb on - strand, within GFF4413 at 50.738 kb on + strand, within GFF4413 at 50.741 kb on + strand, within GFF4413 at 50.741 kb on + strand, within GFF4413 at 50.741 kb on + strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.742 kb on - strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.874 kb on + strand, within GFF4413 at 50.875 kb on - strand, within GFF4413 at 50.875 kb on - strand, within GFF4413 at 50.875 kb on - strand, within GFF4413 at 50.875 kb on - strand, within GFF4413 at 50.875 kb on - strand, within GFF4413 at 50.875 kb on - strand, within GFF4413 at 50.875 kb on - strand, within GFF4413 at 50.875 kb on - strand, within GFF4413 at 51.069 kb on - strand, within GFF4414 at 51.337 kb on + strand, within GFF4414 at 51.337 kb on + strand, within GFF4414 at 51.338 kb on - strand, within GFF4414 at 51.338 kb on - strand, within GFF4414 at 51.338 kb on - strand, within GFF4414 at 51.474 kb on - strand, within GFF4414 at 51.474 kb on - strand, within GFF4414 at 51.474 kb on - strand, within GFF4414 at 51.474 kb on - strand, within GFF4414 at 51.889 kb on + strand, within GFF4414 at 51.983 kb on + strand at 51.984 kb on - strand at 51.984 kb on - strand at 52.045 kb on + strand at 52.045 kb on + strand at 52.045 kb on + strand at 52.100 kb on + strand, within GFF4415 at 52.100 kb on + strand, within GFF4415 at 52.396 kb on + strand at 52.396 kb on + strand at 52.411 kb on + strand at 52.411 kb on + strand at 52.498 kb on + strand, within GFF4416 at 52.591 kb on - strand, within GFF4416 at 52.940 kb on + strand, within GFF4417 at 52.941 kb on - strand, within GFF4417
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 50,026 + GFF4412 0.78 -0.6 50,026 + GFF4412 0.78 -0.0 50,026 + GFF4412 0.78 -0.4 50,026 + GFF4412 0.78 +0.0 50,026 + GFF4412 0.78 +1.3 50,026 + GFF4412 0.78 -2.2 50,026 + GFF4412 0.78 -0.2 50,026 + GFF4412 0.78 +0.0 50,026 + GFF4412 0.78 -1.4 50,026 + GFF4412 0.78 -1.6 50,026 + GFF4412 0.78 -0.9 50,027 - GFF4412 0.78 -2.3 50,027 - GFF4412 0.78 -1.9 50,027 - GFF4412 0.78 -0.2 50,027 - GFF4412 0.78 +0.5 50,027 - GFF4412 0.78 -1.8 50,027 - GFF4412 0.78 -2.1 50,027 - GFF4412 0.78 -1.3 50,027 - GFF4412 0.78 +0.9 50,027 - GFF4412 0.78 +0.9 50,091 + GFF4412 0.81 -0.3 50,091 + GFF4412 0.81 +0.3 50,091 + GFF4412 0.81 -1.1 50,092 - GFF4412 0.81 -0.9 50,092 - GFF4412 0.81 -0.2 50,092 - GFF4412 0.81 -0.8 50,092 - GFF4412 0.81 +0.5 50,094 + GFF4412 0.81 +0.1 50,094 + GFF4412 0.81 +0.4 50,094 + GFF4412 0.81 -0.5 50,094 + GFF4412 0.81 -0.2 50,166 + GFF4412 0.85 -0.1 50,166 + GFF4412 0.85 -0.7 50,167 - GFF4412 0.85 -0.9 50,167 - GFF4412 0.85 -0.4 50,311 + -1.3 50,311 + -0.8 50,311 + -2.3 50,311 + -0.2 50,312 - +0.2 50,312 - +0.5 50,312 - +0.7 50,312 - +0.4 50,328 + -0.4 50,328 + +1.3 50,328 + -0.1 50,328 + -0.4 50,328 + -0.6 50,328 + +0.7 50,329 - -0.9 50,329 - +0.1 50,329 - -1.3 50,476 + -0.4 50,476 + -0.4 50,476 + -1.7 50,476 + +0.1 50,476 + +1.0 50,476 + -0.8 50,476 + -1.6 50,476 + +0.8 50,476 + -1.1 50,476 + +0.4 50,476 + +1.5 50,476 + +1.1 50,477 - -0.5 50,477 - +0.7 50,477 - -1.0 50,477 - -1.5 50,477 - +0.7 50,477 - +0.1 50,477 - -0.6 50,477 - +0.0 50,477 - +0.2 50,477 - +0.6 50,477 - -0.3 50,477 - -1.1 50,498 + -1.3 50,499 - +0.1 50,515 + -0.7 50,515 + +0.0 50,686 + GFF4413 0.44 -0.2 50,686 + GFF4413 0.44 +0.1 50,686 + GFF4413 0.44 +0.2 50,687 - GFF4413 0.44 -0.6 50,687 - GFF4413 0.44 -0.3 50,687 - GFF4413 0.44 -0.1 50,687 - GFF4413 0.44 -1.9 50,738 + GFF4413 0.55 -0.5 50,741 + GFF4413 0.55 -0.2 50,741 + GFF4413 0.55 +0.7 50,741 + GFF4413 0.55 -0.1 50,742 - GFF4413 0.56 +0.5 50,742 - GFF4413 0.56 +0.0 50,742 - GFF4413 0.56 -1.6 50,742 - GFF4413 0.56 -0.3 50,742 - GFF4413 0.56 -0.4 50,742 - GFF4413 0.56 +0.4 50,742 - GFF4413 0.56 -0.5 50,742 - GFF4413 0.56 -0.2 50,742 - GFF4413 0.56 -0.9 50,742 - GFF4413 0.56 -0.0 50,742 - GFF4413 0.56 -0.5 50,742 - GFF4413 0.56 -0.6 50,874 + GFF4413 0.83 +0.9 50,874 + GFF4413 0.83 -0.8 50,874 + GFF4413 0.83 -0.3 50,874 + GFF4413 0.83 -0.4 50,874 + GFF4413 0.83 -2.1 50,874 + GFF4413 0.83 -1.6 50,874 + GFF4413 0.83 -1.1 50,874 + GFF4413 0.83 -1.0 50,874 + GFF4413 0.83 -0.8 50,875 - GFF4413 0.83 -0.3 50,875 - GFF4413 0.83 -1.3 50,875 - GFF4413 0.83 -0.4 50,875 - GFF4413 0.83 +0.3 50,875 - GFF4413 0.83 -0.0 50,875 - GFF4413 0.83 +0.6 50,875 - GFF4413 0.83 -1.8 50,875 - GFF4413 0.83 -0.1 51,069 - GFF4414 0.11 -0.0 51,337 + GFF4414 0.36 +0.7 51,337 + GFF4414 0.36 +0.4 51,338 - GFF4414 0.36 +0.2 51,338 - GFF4414 0.36 +1.0 51,338 - GFF4414 0.36 +1.7 51,474 - GFF4414 0.49 -0.7 51,474 - GFF4414 0.49 +0.7 51,474 - GFF4414 0.49 -0.5 51,474 - GFF4414 0.49 +0.2 51,889 + GFF4414 0.88 -0.8 51,983 + +1.7 51,984 - +0.4 51,984 - -2.2 52,045 + -1.6 52,045 + -0.5 52,045 + -0.4 52,100 + GFF4415 0.23 -0.1 52,100 + GFF4415 0.23 -0.4 52,396 + -0.0 52,396 + -0.8 52,411 + -0.2 52,411 + -0.5 52,498 + GFF4416 0.37 -0.2 52,591 - GFF4416 0.71 -0.8 52,940 + GFF4417 0.10 +0.8 52,941 - GFF4417 0.10 -0.5
Or see this region's nucleotide sequence