Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4181 and GFF4182 are separated by 2 nucleotides GFF4182 and GFF4183 are separated by 115 nucleotides GFF4183 and GFF4184 are separated by 204 nucleotides
GFF4181 - hypothetical protein, at 86,694 to 87,257
GFF4181
GFF4182 - Extracytoplasmic function (ECF) sigma factor VreI, at 87,260 to 87,775
GFF4182
GFF4183 - hypothetical protein, at 87,891 to 88,889
GFF4183
GFF4184 - Filamentous haemagglutinin family outer membrane protein associated with VreARI signalling system, at 89,094 to 102,362
GFF4184
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-2
-1
0
1 at 87.024 kb on + strand, within GFF4181 at 87.024 kb on + strand, within GFF4181 at 87.024 kb on + strand, within GFF4181 at 87.025 kb on - strand, within GFF4181 at 87.308 kb on + strand at 87.308 kb on + strand at 87.309 kb on - strand at 87.437 kb on + strand, within GFF4182 at 87.438 kb on - strand, within GFF4182 at 87.717 kb on - strand, within GFF4182 at 88.053 kb on + strand, within GFF4183 at 88.053 kb on + strand, within GFF4183 at 88.054 kb on - strand, within GFF4183 at 88.054 kb on - strand, within GFF4183 at 88.054 kb on - strand, within GFF4183 at 88.448 kb on + strand, within GFF4183 at 88.605 kb on + strand, within GFF4183 at 88.606 kb on - strand, within GFF4183 at 88.729 kb on - strand, within GFF4183 at 89.153 kb on - strand at 89.874 kb on - strand at 89.874 kb on - strand at 89.877 kb on + strand at 89.877 kb on + strand at 89.878 kb on - strand at 89.878 kb on - strand at 89.878 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 87,024 + GFF4181 0.59 -1.4 87,024 + GFF4181 0.59 -1.9 87,024 + GFF4181 0.59 -0.5 87,025 - GFF4181 0.59 -0.9 87,308 + -0.7 87,308 + +0.1 87,309 - +0.1 87,437 + GFF4182 0.34 -1.4 87,438 - GFF4182 0.34 -0.4 87,717 - GFF4182 0.89 +0.7 88,053 + GFF4183 0.16 -0.5 88,053 + GFF4183 0.16 +0.5 88,054 - GFF4183 0.16 +1.3 88,054 - GFF4183 0.16 +0.3 88,054 - GFF4183 0.16 -0.4 88,448 + GFF4183 0.56 -0.4 88,605 + GFF4183 0.71 -0.0 88,606 - GFF4183 0.72 +0.7 88,729 - GFF4183 0.84 +0.7 89,153 - +1.0 89,874 - +0.5 89,874 - +0.2 89,877 + +0.4 89,877 + -0.1 89,878 - +0.1 89,878 - -1.4 89,878 - -0.1
Or see this region's nucleotide sequence