Strain Fitness in Variovorax sp. SCN45 around GFF4176

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4174 and GFF4175 are separated by 45 nucleotidesGFF4175 and GFF4176 are separated by 90 nucleotidesGFF4176 and GFF4177 overlap by 4 nucleotidesGFF4177 and GFF4178 are separated by 11 nucleotides GFF4174 - FIG00919961: hypothetical protein, at 79,327 to 80,310 GFF4174 GFF4175 - FMN reductase (NADH) (EC 1.5.1.42), at 80,356 to 80,949 GFF4175 GFF4176 - Transcriptional activator MetR, at 81,040 to 81,939 GFF4176 GFF4177 - Epoxyqueuosine reductase (EC 1.17.99.6) QueH, at 81,936 to 82,160 GFF4177 GFF4178 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 82,172 to 83,029 GFF4178 Position (kb) 81 82Strain fitness (log2 ratio) -2 -1 0 1 2at 80.108 kb on + strand, within GFF4174at 80.109 kb on - strand, within GFF4174at 80.212 kb on + strandat 80.212 kb on + strandat 80.213 kb on - strandat 80.213 kb on - strandat 80.258 kb on + strandat 80.258 kb on + strandat 80.258 kb on + strandat 80.338 kb on + strandat 80.338 kb on + strandat 80.338 kb on + strandat 80.339 kb on - strandat 80.339 kb on - strandat 80.339 kb on - strandat 80.339 kb on - strandat 80.675 kb on + strand, within GFF4175at 80.675 kb on + strand, within GFF4175at 80.676 kb on - strand, within GFF4175at 80.676 kb on - strand, within GFF4175at 80.676 kb on - strand, within GFF4175at 80.917 kb on + strandat 80.918 kb on - strandat 80.918 kb on - strandat 80.918 kb on - strandat 80.996 kb on + strandat 81.230 kb on - strand, within GFF4176at 81.349 kb on + strand, within GFF4176at 81.349 kb on + strand, within GFF4176at 81.529 kb on + strand, within GFF4176at 81.529 kb on + strand, within GFF4176at 81.530 kb on - strand, within GFF4176at 81.581 kb on - strand, within GFF4176at 81.623 kb on - strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.752 kb on + strand, within GFF4176at 81.784 kb on + strand, within GFF4176at 81.784 kb on + strand, within GFF4176at 81.883 kb on + strandat 81.883 kb on + strandat 81.884 kb on - strandat 81.966 kb on + strand, within GFF4177at 81.966 kb on + strand, within GFF4177at 81.978 kb on + strand, within GFF4177at 81.978 kb on + strand, within GFF4177at 81.978 kb on + strand, within GFF4177at 81.979 kb on - strand, within GFF4177at 81.979 kb on - strand, within GFF4177at 81.979 kb on - strand, within GFF4177at 82.065 kb on + strand, within GFF4177at 82.066 kb on - strand, within GFF4177at 82.066 kb on - strand, within GFF4177at 82.206 kb on - strandat 82.284 kb on - strand, within GFF4178at 82.292 kb on + strand, within GFF4178at 82.292 kb on + strand, within GFF4178at 82.701 kb on + strand, within GFF4178at 82.701 kb on + strand, within GFF4178at 82.701 kb on + strand, within GFF4178at 82.701 kb on + strand, within GFF4178at 82.702 kb on - strand, within GFF4178at 82.702 kb on - strand, within GFF4178at 82.785 kb on + strand, within GFF4178at 82.786 kb on - strand, within GFF4178at 82.796 kb on + strand, within GFF4178at 82.844 kb on + strand, within GFF4178

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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80,108 + GFF4174 0.79 +0.0
80,109 - GFF4174 0.79 +2.0
80,212 + +0.1
80,212 + +0.7
80,213 - +1.4
80,213 - -0.4
80,258 + -0.3
80,258 + +0.5
80,258 + +0.9
80,338 + -0.4
80,338 + -0.6
80,338 + +1.0
80,339 - +0.0
80,339 - -0.1
80,339 - -0.3
80,339 - -0.2
80,675 + GFF4175 0.54 +1.1
80,675 + GFF4175 0.54 +0.4
80,676 - GFF4175 0.54 +0.2
80,676 - GFF4175 0.54 +0.2
80,676 - GFF4175 0.54 -2.6
80,917 + -0.3
80,918 - -0.5
80,918 - +0.7
80,918 - +0.1
80,996 + -0.1
81,230 - GFF4176 0.21 -0.3
81,349 + GFF4176 0.34 +0.7
81,349 + GFF4176 0.34 +0.7
81,529 + GFF4176 0.54 +0.2
81,529 + GFF4176 0.54 +1.4
81,530 - GFF4176 0.54 +0.0
81,581 - GFF4176 0.60 +0.4
81,623 - GFF4176 0.65 +0.4
81,652 + GFF4176 0.68 -0.4
81,652 + GFF4176 0.68 +0.6
81,652 + GFF4176 0.68 +0.5
81,652 + GFF4176 0.68 +0.4
81,652 + GFF4176 0.68 +0.0
81,652 + GFF4176 0.68 +0.5
81,652 + GFF4176 0.68 -0.5
81,652 + GFF4176 0.68 -0.1
81,652 + GFF4176 0.68 -1.5
81,652 + GFF4176 0.68 +0.1
81,652 + GFF4176 0.68 +0.9
81,652 + GFF4176 0.68 -0.4
81,652 + GFF4176 0.68 +0.6
81,652 + GFF4176 0.68 +0.4
81,653 - GFF4176 0.68 -0.6
81,653 - GFF4176 0.68 -0.5
81,653 - GFF4176 0.68 -0.4
81,653 - GFF4176 0.68 -0.2
81,653 - GFF4176 0.68 -0.3
81,653 - GFF4176 0.68 -0.7
81,653 - GFF4176 0.68 +0.9
81,653 - GFF4176 0.68 -0.6
81,653 - GFF4176 0.68 +0.9
81,653 - GFF4176 0.68 +0.8
81,653 - GFF4176 0.68 -0.1
81,653 - GFF4176 0.68 -2.0
81,653 - GFF4176 0.68 +0.1
81,653 - GFF4176 0.68 -1.0
81,752 + GFF4176 0.79 +1.2
81,784 + GFF4176 0.83 -0.8
81,784 + GFF4176 0.83 -0.6
81,883 + -0.5
81,883 + +0.4
81,884 - -0.8
81,966 + GFF4177 0.13 +0.1
81,966 + GFF4177 0.13 +0.8
81,978 + GFF4177 0.19 +1.3
81,978 + GFF4177 0.19 -0.6
81,978 + GFF4177 0.19 -1.8
81,979 - GFF4177 0.19 -1.4
81,979 - GFF4177 0.19 +0.4
81,979 - GFF4177 0.19 +0.2
82,065 + GFF4177 0.57 +0.9
82,066 - GFF4177 0.58 +1.0
82,066 - GFF4177 0.58 -0.1
82,206 - +0.4
82,284 - GFF4178 0.13 -0.3
82,292 + GFF4178 0.14 +0.5
82,292 + GFF4178 0.14 -0.2
82,701 + GFF4178 0.62 -0.2
82,701 + GFF4178 0.62 -1.7
82,701 + GFF4178 0.62 +0.6
82,701 + GFF4178 0.62 +0.3
82,702 - GFF4178 0.62 -0.3
82,702 - GFF4178 0.62 +1.1
82,785 + GFF4178 0.71 -0.3
82,786 - GFF4178 0.72 -0.6
82,796 + GFF4178 0.73 +0.1
82,844 + GFF4178 0.78 -0.7

Or see this region's nucleotide sequence