Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4058 and GFF4059 are separated by 137 nucleotides GFF4059 and GFF4060 are separated by 263 nucleotides GFF4060 and GFF4061 are separated by 946 nucleotides
GFF4058 - Copper/silver efflux RND transporter, outer membrane protein CusC, at 271,644 to 272,999
GFF4058
GFF4059 - hypothetical protein, at 273,137 to 273,583
GFF4059
GFF4060 - hypothetical protein, at 273,847 to 274,296
GFF4060
GFF4061 - Multicopper oxidase, at 275,243 to 277,054
GFF4061
Position (kb) 273
274
275 Strain fitness (log2 ratio) -2
-1
0
1 at 273.024 kb on + strand at 273.258 kb on + strand, within GFF4059 at 273.259 kb on - strand, within GFF4059 at 273.259 kb on - strand, within GFF4059 at 273.425 kb on + strand, within GFF4059 at 273.454 kb on + strand, within GFF4059 at 273.455 kb on - strand, within GFF4059 at 273.455 kb on - strand, within GFF4059 at 273.455 kb on - strand, within GFF4059 at 273.505 kb on - strand, within GFF4059 at 273.733 kb on + strand at 273.948 kb on - strand, within GFF4060 at 274.217 kb on + strand, within GFF4060 at 274.217 kb on + strand, within GFF4060 at 274.217 kb on + strand, within GFF4060 at 274.217 kb on + strand, within GFF4060 at 274.218 kb on - strand, within GFF4060 at 274.218 kb on - strand, within GFF4060 at 274.218 kb on - strand, within GFF4060 at 274.515 kb on + strand at 274.515 kb on + strand at 274.598 kb on - strand at 274.635 kb on - strand at 274.635 kb on - strand at 274.838 kb on + strand at 274.838 kb on + strand at 274.838 kb on + strand at 274.839 kb on - strand at 274.844 kb on + strand at 274.844 kb on + strand at 274.844 kb on + strand at 274.847 kb on - strand at 274.860 kb on + strand at 275.109 kb on + strand at 275.111 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 273,024 + -0.5 273,258 + GFF4059 0.27 -0.3 273,259 - GFF4059 0.27 -0.8 273,259 - GFF4059 0.27 +0.6 273,425 + GFF4059 0.64 -0.5 273,454 + GFF4059 0.71 +1.0 273,455 - GFF4059 0.71 +0.6 273,455 - GFF4059 0.71 +0.1 273,455 - GFF4059 0.71 -0.0 273,505 - GFF4059 0.82 -1.0 273,733 + -0.7 273,948 - GFF4060 0.22 +0.6 274,217 + GFF4060 0.82 +0.3 274,217 + GFF4060 0.82 -1.9 274,217 + GFF4060 0.82 +0.4 274,217 + GFF4060 0.82 +0.5 274,218 - GFF4060 0.82 +0.3 274,218 - GFF4060 0.82 +0.0 274,218 - GFF4060 0.82 +0.8 274,515 + +0.2 274,515 + +1.6 274,598 - -0.2 274,635 - -0.3 274,635 - -0.1 274,838 + +0.0 274,838 + +0.3 274,838 + -0.0 274,839 - -0.1 274,844 + +0.6 274,844 + -1.1 274,844 + -1.4 274,847 - +0.8 274,860 + -0.1 275,109 + -0.0 275,111 + -2.5
Or see this region's nucleotide sequence