Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF359 and GFF360 overlap by 4 nucleotides GFF360 and GFF361 are separated by 131 nucleotides
GFF359 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 389,167 to 390,174
GFF359
GFF360 - Putative transporter, at 390,171 to 391,328
GFF360
GFF361 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 391,460 to 392,980
GFF361
Position (kb) 390
391
392 Strain fitness (log2 ratio) -2
-1
0
1
2 at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.191 kb on + strand at 389.191 kb on + strand at 389.191 kb on + strand at 389.192 kb on - strand at 389.192 kb on - strand at 389.192 kb on - strand at 389.239 kb on + strand at 389.239 kb on + strand at 389.239 kb on + strand at 389.240 kb on - strand at 389.240 kb on - strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.650 kb on + strand, within GFF359 at 389.681 kb on - strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.073 kb on + strand, within GFF359 at 390.073 kb on + strand, within GFF359 at 390.074 kb on - strand at 390.074 kb on - strand at 390.074 kb on - strand at 390.074 kb on - strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.335 kb on - strand, within GFF360 at 390.335 kb on - strand, within GFF360 at 390.417 kb on + strand, within GFF360 at 390.417 kb on + strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.846 kb on + strand, within GFF360 at 390.847 kb on - strand, within GFF360 at 390.847 kb on - strand, within GFF360 at 390.847 kb on - strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.345 kb on - strand at 391.431 kb on - strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.491 kb on + strand at 391.491 kb on + strand at 391.492 kb on - strand at 391.492 kb on - strand at 391.492 kb on - strand at 391.664 kb on + strand, within GFF361 at 391.664 kb on + strand, within GFF361 at 391.664 kb on + strand, within GFF361 at 391.664 kb on + strand, within GFF361 at 391.665 kb on - strand, within GFF361 at 391.665 kb on - strand, within GFF361 at 391.993 kb on + strand, within GFF361 at 391.993 kb on + strand, within GFF361 at 392.052 kb on - strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.216 kb on + strand, within GFF361 at 392.216 kb on + strand, within GFF361 at 392.216 kb on + strand, within GFF361 at 392.217 kb on - strand, within GFF361 at 392.217 kb on - strand, within GFF361 at 392.306 kb on + strand, within GFF361 at 392.306 kb on + strand, within GFF361 at 392.306 kb on + strand, within GFF361 at 392.307 kb on - strand, within GFF361 at 392.307 kb on - strand, within GFF361 at 392.312 kb on + strand, within GFF361 at 392.313 kb on - strand, within GFF361
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 389,172 + +0.9 389,172 + -0.2 389,172 + -0.6 389,172 + -0.0 389,172 + +0.4 389,172 + -0.7 389,173 - +1.4 389,173 - +0.3 389,173 - -0.3 389,173 - -0.2 389,173 - -0.3 389,173 - -0.1 389,173 - +0.0 389,173 - -1.0 389,173 - +0.1 389,191 + -1.1 389,191 + +0.5 389,191 + -0.3 389,192 - +0.1 389,192 - +0.4 389,192 - -1.0 389,239 + -0.1 389,239 + -1.1 389,239 + -0.1 389,240 - -0.3 389,240 - -0.0 389,254 + -0.4 389,254 + -0.4 389,254 + -0.5 389,254 + +1.9 389,254 + -0.6 389,254 + +1.6 389,254 + +0.0 389,254 + +0.1 389,255 - -0.8 389,255 - +0.0 389,255 - -1.2 389,255 - -1.8 389,255 - +0.1 389,255 - -0.4 389,255 - +1.2 389,255 - -0.3 389,256 + -0.6 389,256 + -0.1 389,256 + +0.5 389,256 + +1.8 389,256 + +0.3 389,256 + +0.4 389,256 + -0.0 389,256 + +0.4 389,257 - +0.2 389,257 - +0.6 389,257 - +0.8 389,257 - -1.5 389,257 - +0.1 389,257 - +0.2 389,257 - -1.6 389,257 - +1.6 389,257 - -0.6 389,257 - -0.8 389,533 + GFF359 0.36 +0.8 389,533 + GFF359 0.36 -0.6 389,533 + GFF359 0.36 -1.8 389,533 + GFF359 0.36 -1.4 389,533 + GFF359 0.36 +1.1 389,533 + GFF359 0.36 -0.5 389,533 + GFF359 0.36 +0.6 389,533 + GFF359 0.36 -1.3 389,533 + GFF359 0.36 +0.2 389,533 + GFF359 0.36 +0.5 389,533 + GFF359 0.36 -0.6 389,533 + GFF359 0.36 -1.0 389,534 - GFF359 0.36 -0.6 389,534 - GFF359 0.36 +0.3 389,534 - GFF359 0.36 -0.2 389,534 - GFF359 0.36 -0.1 389,534 - GFF359 0.36 -0.2 389,534 - GFF359 0.36 -0.5 389,534 - GFF359 0.36 -0.1 389,534 - GFF359 0.36 -1.8 389,534 - GFF359 0.36 -0.1 389,534 - GFF359 0.36 -0.1 389,534 - GFF359 0.36 +0.1 389,650 + GFF359 0.48 -0.3 389,681 - GFF359 0.51 -0.6 390,055 + GFF359 0.88 -0.8 390,055 + GFF359 0.88 -1.3 390,055 + GFF359 0.88 +0.0 390,056 - GFF359 0.88 -0.3 390,056 - GFF359 0.88 -1.9 390,056 - GFF359 0.88 -0.4 390,056 - GFF359 0.88 +0.1 390,073 + GFF359 0.90 -0.2 390,073 + GFF359 0.90 -0.8 390,074 - +0.4 390,074 - +0.1 390,074 - -0.3 390,074 - -0.4 390,082 + -0.2 390,082 + +2.4 390,082 + -1.1 390,082 + -0.5 390,083 - +0.4 390,083 - +0.4 390,083 - -0.8 390,083 - -0.3 390,083 - +0.6 390,083 - +0.3 390,083 - -0.6 390,115 + -0.4 390,115 + -1.4 390,115 + -0.0 390,115 + -0.4 390,115 + -0.0 390,115 + -0.5 390,115 + -0.1 390,115 + -0.2 390,115 + +0.0 390,115 + +0.4 390,115 + -0.2 390,115 + -1.0 390,116 - -0.3 390,116 - +0.3 390,116 - -0.0 390,116 - +0.2 390,116 - -0.5 390,116 - +0.5 390,116 - -1.1 390,116 - -1.4 390,116 - +0.9 390,116 - -0.8 390,116 - -0.6 390,116 - -0.4 390,116 - -2.1 390,335 - GFF360 0.14 +1.0 390,335 - GFF360 0.14 -0.8 390,417 + GFF360 0.21 +0.7 390,417 + GFF360 0.21 +0.0 390,418 - GFF360 0.21 +1.5 390,418 - GFF360 0.21 -2.5 390,418 - GFF360 0.21 -0.2 390,646 + GFF360 0.41 -0.9 390,646 + GFF360 0.41 -0.4 390,646 + GFF360 0.41 +0.4 390,646 + GFF360 0.41 +0.4 390,646 + GFF360 0.41 -1.0 390,646 + GFF360 0.41 -0.6 390,647 - GFF360 0.41 -1.3 390,647 - GFF360 0.41 +0.2 390,647 - GFF360 0.41 -0.3 390,647 - GFF360 0.41 -0.3 390,647 - GFF360 0.41 -0.6 390,647 - GFF360 0.41 -0.2 390,647 - GFF360 0.41 +1.4 390,647 - GFF360 0.41 +0.5 390,647 - GFF360 0.41 -1.5 390,647 - GFF360 0.41 +0.8 390,846 + GFF360 0.58 +0.6 390,847 - GFF360 0.58 +0.3 390,847 - GFF360 0.58 +0.1 390,847 - GFF360 0.58 -0.2 391,182 + GFF360 0.87 +0.6 391,182 + GFF360 0.87 -0.3 391,182 + GFF360 0.87 +0.2 391,182 + GFF360 0.87 -0.1 391,182 + GFF360 0.87 -0.4 391,182 + GFF360 0.87 +1.4 391,182 + GFF360 0.87 -1.1 391,182 + GFF360 0.87 -0.0 391,182 + GFF360 0.87 +0.3 391,183 - GFF360 0.87 -1.4 391,183 - GFF360 0.87 +0.8 391,183 - GFF360 0.87 +0.9 391,183 - GFF360 0.87 -1.0 391,183 - GFF360 0.87 +0.4 391,183 - GFF360 0.87 +0.6 391,183 - GFF360 0.87 -1.0 391,183 - GFF360 0.87 -1.2 391,183 - GFF360 0.87 +2.4 391,345 - -0.1 391,431 - +0.1 391,472 + +0.9 391,472 + -0.3 391,472 + -0.5 391,472 + -0.7 391,472 + -1.1 391,472 + +1.9 391,472 + -0.7 391,472 + -0.2 391,473 - -2.0 391,473 - +0.4 391,473 - +0.8 391,473 - -0.4 391,473 - +1.1 391,473 - +0.4 391,491 + -0.2 391,491 + -0.3 391,492 - -0.4 391,492 - -0.9 391,492 - -0.2 391,664 + GFF361 0.13 +0.8 391,664 + GFF361 0.13 +1.4 391,664 + GFF361 0.13 +0.2 391,664 + GFF361 0.13 -0.6 391,665 - GFF361 0.13 -0.2 391,665 - GFF361 0.13 +0.1 391,993 + GFF361 0.35 -1.8 391,993 + GFF361 0.35 -1.2 392,052 - GFF361 0.39 -0.1 392,207 + GFF361 0.49 -1.0 392,207 + GFF361 0.49 +1.6 392,207 + GFF361 0.49 -1.4 392,207 + GFF361 0.49 -0.1 392,207 + GFF361 0.49 +1.9 392,207 + GFF361 0.49 +0.6 392,207 + GFF361 0.49 -1.1 392,208 - GFF361 0.49 +0.0 392,208 - GFF361 0.49 -0.5 392,208 - GFF361 0.49 -2.0 392,208 - GFF361 0.49 -0.1 392,208 - GFF361 0.49 -0.6 392,208 - GFF361 0.49 +0.8 392,208 - GFF361 0.49 -0.0 392,208 - GFF361 0.49 +0.1 392,208 - GFF361 0.49 -0.1 392,208 - GFF361 0.49 +0.0 392,208 - GFF361 0.49 +0.5 392,216 + GFF361 0.50 -0.3 392,216 + GFF361 0.50 +0.7 392,216 + GFF361 0.50 -1.6 392,217 - GFF361 0.50 +0.1 392,217 - GFF361 0.50 -0.6 392,306 + GFF361 0.56 -0.0 392,306 + GFF361 0.56 -0.4 392,306 + GFF361 0.56 +0.4 392,307 - GFF361 0.56 +0.7 392,307 - GFF361 0.56 -0.5 392,312 + GFF361 0.56 -1.6 392,313 - GFF361 0.56 -0.6
Or see this region's nucleotide sequence