Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3345 and GFF3346 overlap by 4 nucleotides GFF3346 and GFF3347 are separated by 121 nucleotides GFF3347 and GFF3348 are separated by 4 nucleotides GFF3348 and GFF3349 are separated by 160 nucleotides
GFF3345 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), glycolate utilization, at 168,956 to 169,555
GFF3345
GFF3346 - hypothetical protein, at 169,552 to 169,914
GFF3346
GFF3347 - Adenylate cyclase (EC 4.6.1.1), at 170,036 to 170,557
GFF3347
GFF3348 - Putative lipase, at 170,562 to 171,203
GFF3348
GFF3349 - Branched-chain amino acid ABC transporter, substrate-binding protein LivJ (TC 3.A.1.4.1), at 171,364 to 172,545
GFF3349
Position (kb) 170
171 Strain fitness (log2 ratio) -1
0
1 at 169.406 kb on + strand, within GFF3345 at 169.481 kb on + strand, within GFF3345 at 169.913 kb on - strand at 169.924 kb on - strand at 169.924 kb on - strand at 170.125 kb on - strand, within GFF3347 at 170.223 kb on - strand, within GFF3347 at 170.261 kb on + strand, within GFF3347 at 170.262 kb on - strand, within GFF3347 at 170.262 kb on - strand, within GFF3347 at 170.528 kb on + strand at 170.529 kb on - strand at 170.618 kb on - strand at 170.680 kb on + strand, within GFF3348 at 170.680 kb on + strand, within GFF3348 at 170.681 kb on - strand, within GFF3348 at 170.681 kb on - strand, within GFF3348 at 170.728 kb on + strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.917 kb on + strand, within GFF3348 at 170.918 kb on - strand, within GFF3348 at 171.217 kb on + strand at 171.218 kb on - strand at 171.269 kb on + strand at 171.269 kb on + strand at 171.410 kb on + strand at 171.518 kb on - strand, within GFF3349 at 171.518 kb on - strand, within GFF3349
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 169,406 + GFF3345 0.75 -0.4 169,481 + GFF3345 0.88 -1.7 169,913 - -1.4 169,924 - +0.9 169,924 - -0.4 170,125 - GFF3347 0.17 -0.3 170,223 - GFF3347 0.36 -0.4 170,261 + GFF3347 0.43 +0.4 170,262 - GFF3347 0.43 -0.4 170,262 - GFF3347 0.43 -1.4 170,528 + -0.1 170,529 - +0.2 170,618 - -0.3 170,680 + GFF3348 0.18 -0.0 170,680 + GFF3348 0.18 +0.2 170,681 - GFF3348 0.19 -1.1 170,681 - GFF3348 0.19 -1.0 170,728 + GFF3348 0.26 +0.0 170,729 - GFF3348 0.26 +0.3 170,729 - GFF3348 0.26 -0.8 170,729 - GFF3348 0.26 +0.7 170,729 - GFF3348 0.26 -0.3 170,729 - GFF3348 0.26 -0.4 170,917 + GFF3348 0.55 -0.2 170,918 - GFF3348 0.55 +0.5 171,217 + +0.1 171,218 - +0.6 171,269 + +1.1 171,269 + -1.2 171,410 + +1.2 171,518 - GFF3349 0.13 -1.1 171,518 - GFF3349 0.13 +1.1
Or see this region's nucleotide sequence