Strain Fitness in Variovorax sp. SCN45 around GFF322

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF319 and GFF320 overlap by 4 nucleotidesGFF320 and GFF321 overlap by 19 nucleotidesGFF321 and GFF322 are separated by 33 nucleotidesGFF322 and GFF323 overlap by 4 nucleotides GFF319 - EXTRACYTOPLASMIC FUNCTION ALTERNATIVE SIGMA FACTOR, at 352,499 to 353,140 GFF319 GFF320 - RNA polymerase ECF-type sigma factor, at 353,137 to 353,727 GFF320 GFF321 - hypothetical protein, at 353,709 to 353,975 GFF321 GFF322 - GTP cyclohydrolase II homolog, at 354,009 to 355,151 GFF322 GFF323 - Possible pyrimidine-degrading protein DUF1688, at 355,148 to 356,554 GFF323 Position (kb) 354 355 356Strain fitness (log2 ratio) -2 -1 0 1 2at 353.283 kb on + strand, within GFF320at 353.283 kb on + strand, within GFF320at 353.283 kb on + strand, within GFF320at 353.284 kb on - strand, within GFF320at 353.284 kb on - strand, within GFF320at 353.447 kb on + strand, within GFF320at 353.447 kb on + strand, within GFF320at 353.447 kb on + strand, within GFF320at 353.448 kb on - strand, within GFF320at 353.448 kb on - strand, within GFF320at 353.495 kb on + strand, within GFF320at 353.495 kb on + strand, within GFF320at 353.495 kb on + strand, within GFF320at 353.495 kb on + strand, within GFF320at 353.496 kb on - strand, within GFF320at 353.496 kb on - strand, within GFF320at 353.496 kb on - strand, within GFF320at 353.710 kb on + strandat 353.710 kb on + strandat 353.710 kb on + strandat 353.711 kb on - strandat 353.711 kb on - strandat 353.711 kb on - strandat 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.069 kb on + strandat 354.069 kb on + strandat 354.069 kb on + strandat 354.069 kb on + strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.079 kb on - strandat 354.079 kb on - strandat 354.261 kb on + strand, within GFF322at 354.261 kb on + strand, within GFF322at 354.417 kb on + strand, within GFF322at 354.418 kb on - strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.681 kb on + strand, within GFF322at 354.681 kb on + strand, within GFF322at 354.682 kb on - strand, within GFF322at 354.798 kb on + strand, within GFF322at 354.798 kb on + strand, within GFF322at 354.798 kb on + strand, within GFF322at 354.799 kb on - strand, within GFF322at 354.799 kb on - strand, within GFF322at 354.799 kb on - strand, within GFF322at 354.843 kb on + strand, within GFF322at 354.844 kb on - strand, within GFF322at 355.080 kb on + strandat 355.081 kb on - strandat 355.081 kb on - strandat 355.242 kb on - strandat 355.242 kb on - strandat 355.613 kb on + strand, within GFF323at 355.613 kb on + strand, within GFF323at 355.613 kb on + strand, within GFF323at 355.614 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.722 kb on - strand, within GFF323at 355.722 kb on - strand, within GFF323

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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353,283 + GFF320 0.25 -1.4
353,283 + GFF320 0.25 -0.8
353,283 + GFF320 0.25 -0.4
353,284 - GFF320 0.25 +1.0
353,284 - GFF320 0.25 -1.1
353,447 + GFF320 0.52 -1.0
353,447 + GFF320 0.52 +0.2
353,447 + GFF320 0.52 -0.2
353,448 - GFF320 0.53 +0.0
353,448 - GFF320 0.53 -2.1
353,495 + GFF320 0.61 -0.8
353,495 + GFF320 0.61 +1.4
353,495 + GFF320 0.61 -0.2
353,495 + GFF320 0.61 +2.1
353,496 - GFF320 0.61 +0.6
353,496 - GFF320 0.61 +1.8
353,496 - GFF320 0.61 +0.6
353,710 + +0.9
353,710 + +0.4
353,710 + +0.0
353,711 - -0.8
353,711 - +0.2
353,711 - -0.3
353,815 + GFF321 0.40 -0.3
353,815 + GFF321 0.40 +0.5
353,815 + GFF321 0.40 -1.1
353,815 + GFF321 0.40 -0.3
353,815 + GFF321 0.40 +0.8
353,815 + GFF321 0.40 -1.8
353,815 + GFF321 0.40 +0.6
353,815 + GFF321 0.40 -0.7
353,816 - GFF321 0.40 -0.5
353,816 - GFF321 0.40 -0.0
353,816 - GFF321 0.40 -1.6
353,816 - GFF321 0.40 +0.0
353,816 - GFF321 0.40 -0.2
353,816 - GFF321 0.40 -0.4
354,049 + +1.3
354,049 + +0.1
354,049 + -1.1
354,049 + -0.2
354,049 + -0.1
354,049 + -1.0
354,049 + +0.4
354,050 - -0.6
354,050 - -1.2
354,050 - -0.1
354,050 - -1.4
354,050 - -0.7
354,050 - -0.3
354,050 - -0.6
354,069 + +0.7
354,069 + -0.6
354,069 + -0.1
354,069 + -0.1
354,070 - +0.4
354,070 - +0.8
354,070 - -0.4
354,070 - +0.5
354,070 - -0.6
354,070 - +0.6
354,070 - +0.1
354,079 - -0.4
354,079 - -0.9
354,261 + GFF322 0.22 -0.3
354,261 + GFF322 0.22 -0.6
354,417 + GFF322 0.36 -0.2
354,418 - GFF322 0.36 -0.2
354,498 + GFF322 0.43 -0.2
354,498 + GFF322 0.43 +0.4
354,498 + GFF322 0.43 -0.8
354,499 - GFF322 0.43 -1.0
354,499 - GFF322 0.43 -0.4
354,499 - GFF322 0.43 -2.1
354,499 - GFF322 0.43 +0.6
354,564 + GFF322 0.49 -1.8
354,564 + GFF322 0.49 +0.0
354,564 + GFF322 0.49 -1.6
354,564 + GFF322 0.49 +1.1
354,564 + GFF322 0.49 -1.1
354,564 + GFF322 0.49 -0.7
354,565 - GFF322 0.49 -0.3
354,565 - GFF322 0.49 -0.3
354,565 - GFF322 0.49 -0.5
354,565 - GFF322 0.49 -0.8
354,565 - GFF322 0.49 +0.0
354,681 + GFF322 0.59 -1.0
354,681 + GFF322 0.59 -0.2
354,682 - GFF322 0.59 -0.7
354,798 + GFF322 0.69 -0.5
354,798 + GFF322 0.69 -1.6
354,798 + GFF322 0.69 -0.3
354,799 - GFF322 0.69 -0.8
354,799 - GFF322 0.69 +0.3
354,799 - GFF322 0.69 -0.1
354,843 + GFF322 0.73 +0.1
354,844 - GFF322 0.73 -0.2
355,080 + -2.0
355,081 - -1.2
355,081 - -0.9
355,242 - -0.8
355,242 - -0.8
355,613 + GFF323 0.33 -1.4
355,613 + GFF323 0.33 +0.6
355,613 + GFF323 0.33 +0.7
355,614 - GFF323 0.33 -0.3
355,674 - GFF323 0.37 +0.3
355,674 - GFF323 0.37 -1.1
355,674 - GFF323 0.37 -0.6
355,674 - GFF323 0.37 -0.4
355,674 - GFF323 0.37 -0.5
355,722 - GFF323 0.41 +1.7
355,722 - GFF323 0.41 +0.0

Or see this region's nucleotide sequence