Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3078 and GFF3079 are separated by 261 nucleotides GFF3079 and GFF3080 are separated by 78 nucleotides GFF3080 and GFF3081 are separated by 33 nucleotides GFF3081 and GFF3082 are separated by 127 nucleotides GFF3082 and GFF3084 are separated by 43 nucleotides
GFF3078 - SAM-dependent methyltransferase, at 511,489 to 513,090
GFF3078
GFF3079 - hypothetical protein, at 513,352 to 513,627
GFF3079
GFF3080 - no description, at 513,706 to 513,840
GFF3080
GFF3081 - no description, at 513,874 to 513,993
GFF3081
GFF3082 - hypothetical protein, at 514,121 to 514,561
GFF3082
GFF3084 - no description, at 514,605 to 514,880
GFF3084
Position (kb) 513
514 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 512.381 kb on + strand, within GFF3078 at 512.381 kb on + strand, within GFF3078 at 512.381 kb on + strand, within GFF3078 at 512.381 kb on + strand, within GFF3078 at 512.382 kb on - strand, within GFF3078 at 512.382 kb on - strand, within GFF3078 at 512.382 kb on - strand, within GFF3078 at 512.382 kb on - strand, within GFF3078 at 512.423 kb on + strand, within GFF3078 at 512.423 kb on + strand, within GFF3078 at 512.424 kb on - strand, within GFF3078 at 512.424 kb on - strand, within GFF3078 at 512.531 kb on + strand, within GFF3078 at 512.531 kb on + strand, within GFF3078 at 512.531 kb on + strand, within GFF3078 at 512.531 kb on + strand, within GFF3078 at 512.532 kb on - strand, within GFF3078 at 512.532 kb on - strand, within GFF3078 at 512.619 kb on - strand, within GFF3078 at 512.619 kb on - strand, within GFF3078 at 512.619 kb on - strand, within GFF3078 at 512.619 kb on - strand, within GFF3078 at 512.627 kb on + strand, within GFF3078 at 512.627 kb on + strand, within GFF3078 at 512.627 kb on + strand, within GFF3078 at 512.627 kb on + strand, within GFF3078 at 512.627 kb on + strand, within GFF3078 at 512.627 kb on + strand, within GFF3078 at 512.628 kb on - strand, within GFF3078 at 512.628 kb on - strand, within GFF3078 at 512.628 kb on - strand, within GFF3078 at 512.636 kb on + strand, within GFF3078 at 512.636 kb on + strand, within GFF3078 at 512.637 kb on - strand, within GFF3078 at 512.637 kb on - strand, within GFF3078 at 512.637 kb on - strand, within GFF3078 at 512.637 kb on - strand, within GFF3078 at 512.714 kb on + strand, within GFF3078 at 512.715 kb on - strand, within GFF3078 at 512.735 kb on + strand, within GFF3078 at 512.735 kb on + strand, within GFF3078 at 512.735 kb on + strand, within GFF3078 at 512.735 kb on + strand, within GFF3078 at 512.736 kb on - strand, within GFF3078 at 512.736 kb on - strand, within GFF3078 at 512.736 kb on - strand, within GFF3078 at 512.736 kb on - strand, within GFF3078 at 512.736 kb on - strand, within GFF3078 at 512.736 kb on - strand, within GFF3078 at 513.033 kb on - strand at 513.033 kb on - strand at 513.047 kb on + strand at 513.047 kb on + strand at 513.047 kb on + strand at 513.047 kb on + strand at 513.047 kb on + strand at 513.048 kb on - strand at 513.048 kb on - strand at 513.048 kb on - strand at 513.050 kb on + strand at 513.050 kb on + strand at 513.050 kb on + strand at 513.051 kb on - strand at 513.101 kb on + strand at 513.101 kb on + strand at 513.101 kb on + strand at 513.101 kb on + strand at 513.102 kb on - strand at 513.102 kb on - strand at 513.102 kb on - strand at 513.102 kb on - strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.120 kb on + strand at 513.120 kb on + strand at 513.120 kb on + strand at 513.120 kb on + strand at 513.120 kb on + strand at 513.120 kb on + strand at 513.120 kb on + strand at 513.120 kb on + strand at 513.121 kb on - strand at 513.121 kb on - strand at 513.121 kb on - strand at 513.121 kb on - strand at 513.176 kb on - strand at 513.313 kb on - strand at 513.317 kb on + strand at 513.318 kb on - strand at 513.318 kb on - strand at 513.326 kb on - strand at 513.505 kb on + strand, within GFF3079 at 513.505 kb on + strand, within GFF3079 at 513.505 kb on + strand, within GFF3079 at 513.506 kb on - strand, within GFF3079 at 513.506 kb on - strand, within GFF3079 at 513.506 kb on - strand, within GFF3079 at 513.580 kb on + strand, within GFF3079 at 513.580 kb on + strand, within GFF3079 at 513.580 kb on + strand, within GFF3079 at 513.580 kb on + strand, within GFF3079 at 513.580 kb on + strand, within GFF3079 at 513.580 kb on + strand, within GFF3079 at 513.580 kb on + strand, within GFF3079 at 513.581 kb on - strand, within GFF3079 at 513.581 kb on - strand, within GFF3079 at 513.581 kb on - strand, within GFF3079 at 513.581 kb on - strand, within GFF3079 at 513.586 kb on + strand, within GFF3079 at 513.587 kb on - strand, within GFF3079 at 513.625 kb on + strand at 513.625 kb on + strand at 513.625 kb on + strand at 513.626 kb on - strand at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.742 kb on + strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.743 kb on - strand, within GFF3080 at 513.761 kb on - strand, within GFF3080 at 513.761 kb on - strand, within GFF3080 at 513.761 kb on - strand, within GFF3080 at 513.761 kb on - strand, within GFF3080 at 513.939 kb on + strand, within GFF3081 at 513.939 kb on + strand, within GFF3081 at 513.939 kb on + strand, within GFF3081 at 513.940 kb on - strand, within GFF3081 at 513.940 kb on - strand, within GFF3081 at 514.070 kb on + strand at 514.070 kb on + strand at 514.070 kb on + strand at 514.145 kb on + strand at 514.145 kb on + strand at 514.146 kb on - strand at 514.230 kb on + strand, within GFF3082 at 514.230 kb on + strand, within GFF3082 at 514.230 kb on + strand, within GFF3082 at 514.230 kb on + strand, within GFF3082 at 514.266 kb on + strand, within GFF3082 at 514.267 kb on - strand, within GFF3082 at 514.267 kb on - strand, within GFF3082 at 514.267 kb on - strand, within GFF3082 at 514.267 kb on - strand, within GFF3082 at 514.537 kb on + strand at 514.538 kb on - strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.576 kb on + strand at 514.576 kb on + strand at 514.576 kb on + strand at 514.576 kb on + strand at 514.577 kb on - strand at 514.577 kb on - strand at 514.585 kb on + strand at 514.585 kb on + strand at 514.585 kb on + strand at 514.585 kb on + strand at 514.585 kb on + strand at 514.586 kb on - strand at 514.586 kb on - strand at 514.586 kb on - strand at 514.606 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 512,381 + GFF3078 0.56 -0.7 512,381 + GFF3078 0.56 -0.4 512,381 + GFF3078 0.56 -0.2 512,381 + GFF3078 0.56 -0.8 512,382 - GFF3078 0.56 +1.3 512,382 - GFF3078 0.56 -0.1 512,382 - GFF3078 0.56 +3.4 512,382 - GFF3078 0.56 +1.0 512,423 + GFF3078 0.58 -0.7 512,423 + GFF3078 0.58 -0.1 512,424 - GFF3078 0.58 +1.0 512,424 - GFF3078 0.58 +0.4 512,531 + GFF3078 0.65 -2.1 512,531 + GFF3078 0.65 -0.5 512,531 + GFF3078 0.65 +1.2 512,531 + GFF3078 0.65 -0.4 512,532 - GFF3078 0.65 +0.3 512,532 - GFF3078 0.65 -0.6 512,619 - GFF3078 0.71 +0.3 512,619 - GFF3078 0.71 -0.4 512,619 - GFF3078 0.71 -0.6 512,619 - GFF3078 0.71 +0.7 512,627 + GFF3078 0.71 -1.6 512,627 + GFF3078 0.71 -0.3 512,627 + GFF3078 0.71 -0.3 512,627 + GFF3078 0.71 -1.1 512,627 + GFF3078 0.71 +0.1 512,627 + GFF3078 0.71 -0.4 512,628 - GFF3078 0.71 -1.8 512,628 - GFF3078 0.71 -0.8 512,628 - GFF3078 0.71 -1.8 512,636 + GFF3078 0.72 +1.0 512,636 + GFF3078 0.72 -1.2 512,637 - GFF3078 0.72 -0.1 512,637 - GFF3078 0.72 -0.3 512,637 - GFF3078 0.72 +0.3 512,637 - GFF3078 0.72 +0.3 512,714 + GFF3078 0.76 -0.6 512,715 - GFF3078 0.77 -0.9 512,735 + GFF3078 0.78 +0.9 512,735 + GFF3078 0.78 -3.6 512,735 + GFF3078 0.78 -1.6 512,735 + GFF3078 0.78 -2.0 512,736 - GFF3078 0.78 -1.0 512,736 - GFF3078 0.78 +0.6 512,736 - GFF3078 0.78 +0.2 512,736 - GFF3078 0.78 -0.3 512,736 - GFF3078 0.78 +0.2 512,736 - GFF3078 0.78 -0.9 513,033 - +0.2 513,033 - -0.5 513,047 + -0.0 513,047 + -0.2 513,047 + -1.0 513,047 + +0.1 513,047 + -0.4 513,048 - +0.0 513,048 - -0.6 513,048 - +0.1 513,050 + +0.2 513,050 + -0.0 513,050 + +0.3 513,051 - -0.8 513,101 + -0.0 513,101 + -0.2 513,101 + +0.6 513,101 + +0.9 513,102 - -0.2 513,102 - -0.6 513,102 - -0.5 513,102 - -0.5 513,118 + +0.4 513,118 + -0.2 513,118 + -2.0 513,118 + -0.8 513,118 + -0.8 513,118 + +0.0 513,119 - -0.2 513,119 - +0.6 513,119 - -1.6 513,119 - -0.5 513,119 - -0.7 513,119 - -0.3 513,119 - -0.7 513,119 - -2.2 513,120 + -0.7 513,120 + +0.9 513,120 + +0.6 513,120 + +0.3 513,120 + -0.8 513,120 + +1.0 513,120 + +0.1 513,120 + +0.4 513,121 - -0.1 513,121 - -0.8 513,121 - +0.0 513,121 - +1.7 513,176 - -1.2 513,313 - +0.5 513,317 + -0.6 513,318 - +0.2 513,318 - +0.4 513,326 - -0.1 513,505 + GFF3079 0.55 -0.4 513,505 + GFF3079 0.55 -2.0 513,505 + GFF3079 0.55 -0.2 513,506 - GFF3079 0.56 -0.6 513,506 - GFF3079 0.56 -0.7 513,506 - GFF3079 0.56 -0.4 513,580 + GFF3079 0.83 -2.2 513,580 + GFF3079 0.83 +0.1 513,580 + GFF3079 0.83 -2.5 513,580 + GFF3079 0.83 -2.1 513,580 + GFF3079 0.83 -0.2 513,580 + GFF3079 0.83 +0.0 513,580 + GFF3079 0.83 -0.6 513,581 - GFF3079 0.83 -1.0 513,581 - GFF3079 0.83 -0.8 513,581 - GFF3079 0.83 -0.0 513,581 - GFF3079 0.83 -0.2 513,586 + GFF3079 0.85 -0.7 513,587 - GFF3079 0.85 +0.6 513,625 + -0.3 513,625 + +1.6 513,625 + -0.7 513,626 - -0.3 513,742 + GFF3080 0.27 -0.1 513,742 + GFF3080 0.27 -0.6 513,742 + GFF3080 0.27 +0.9 513,742 + GFF3080 0.27 +0.2 513,742 + GFF3080 0.27 +0.2 513,742 + GFF3080 0.27 +0.3 513,742 + GFF3080 0.27 +1.0 513,742 + GFF3080 0.27 +0.2 513,742 + GFF3080 0.27 -0.8 513,742 + GFF3080 0.27 -1.6 513,742 + GFF3080 0.27 -0.3 513,742 + GFF3080 0.27 -0.9 513,742 + GFF3080 0.27 -0.6 513,742 + GFF3080 0.27 +0.3 513,742 + GFF3080 0.27 -1.4 513,742 + GFF3080 0.27 -0.8 513,742 + GFF3080 0.27 -0.2 513,742 + GFF3080 0.27 +0.2 513,742 + GFF3080 0.27 +0.9 513,742 + GFF3080 0.27 +0.0 513,742 + GFF3080 0.27 -1.7 513,742 + GFF3080 0.27 -0.2 513,743 - GFF3080 0.27 -0.2 513,743 - GFF3080 0.27 -0.6 513,743 - GFF3080 0.27 -1.7 513,743 - GFF3080 0.27 -0.7 513,743 - GFF3080 0.27 +1.2 513,743 - GFF3080 0.27 +0.3 513,743 - GFF3080 0.27 -0.7 513,743 - GFF3080 0.27 -0.4 513,743 - GFF3080 0.27 -0.6 513,743 - GFF3080 0.27 -0.4 513,743 - GFF3080 0.27 -0.4 513,743 - GFF3080 0.27 -1.6 513,743 - GFF3080 0.27 -0.3 513,743 - GFF3080 0.27 -0.3 513,743 - GFF3080 0.27 +0.3 513,743 - GFF3080 0.27 -0.3 513,743 - GFF3080 0.27 -0.1 513,743 - GFF3080 0.27 +1.3 513,743 - GFF3080 0.27 -0.6 513,743 - GFF3080 0.27 -0.7 513,743 - GFF3080 0.27 -0.7 513,761 - GFF3080 0.41 +0.3 513,761 - GFF3080 0.41 +0.2 513,761 - GFF3080 0.41 -2.0 513,761 - GFF3080 0.41 -1.6 513,939 + GFF3081 0.54 +0.9 513,939 + GFF3081 0.54 -0.5 513,939 + GFF3081 0.54 +0.4 513,940 - GFF3081 0.55 -0.5 513,940 - GFF3081 0.55 -0.5 514,070 + -0.5 514,070 + -0.8 514,070 + +0.3 514,145 + +0.1 514,145 + +0.1 514,146 - +0.2 514,230 + GFF3082 0.25 -0.4 514,230 + GFF3082 0.25 -0.5 514,230 + GFF3082 0.25 -0.5 514,230 + GFF3082 0.25 +0.0 514,266 + GFF3082 0.33 -0.0 514,267 - GFF3082 0.33 +0.2 514,267 - GFF3082 0.33 +0.9 514,267 - GFF3082 0.33 -0.2 514,267 - GFF3082 0.33 -0.8 514,537 + -0.5 514,538 - -0.2 514,561 + +0.7 514,561 + +0.1 514,561 + -0.4 514,576 + -1.2 514,576 + -0.6 514,576 + +0.0 514,576 + -1.0 514,577 - +0.5 514,577 - -0.4 514,585 + +0.0 514,585 + +0.8 514,585 + +0.3 514,585 + +0.1 514,585 + +0.3 514,586 - +3.2 514,586 - +1.6 514,586 - -0.1 514,606 + -1.0
Or see this region's nucleotide sequence