Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2969 and GFF2970 overlap by 4 nucleotides GFF2970 and GFF2971 are separated by 130 nucleotides
GFF2969 - acyl-CoA synthetase (NDP forming type), at 400,055 to 402,211
GFF2969
GFF2970 - BUG/TctC family periplasmic protein, at 402,208 to 403,194
GFF2970
GFF2971 - Transcriptional regulator, LysR family, at 403,325 to 404,290
GFF2971
Position (kb) 402
403
404 Strain fitness (log2 ratio) -2
-1
0
1
2 at 401.266 kb on - strand, within GFF2969 at 401.515 kb on - strand, within GFF2969 at 401.515 kb on - strand, within GFF2969 at 401.659 kb on - strand, within GFF2969 at 401.736 kb on + strand, within GFF2969 at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.070 kb on - strand at 402.070 kb on - strand at 402.311 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.671 kb on + strand, within GFF2970 at 402.671 kb on + strand, within GFF2970 at 402.672 kb on - strand, within GFF2970 at 402.672 kb on - strand, within GFF2970 at 402.832 kb on + strand, within GFF2970 at 402.833 kb on - strand, within GFF2970 at 402.854 kb on + strand, within GFF2970 at 402.854 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.870 kb on - strand, within GFF2970 at 402.917 kb on + strand, within GFF2970 at 403.101 kb on - strand at 403.101 kb on - strand at 403.262 kb on + strand at 403.263 kb on - strand at 403.263 kb on - strand at 403.263 kb on - strand at 403.274 kb on + strand at 403.276 kb on + strand at 403.289 kb on - strand at 403.312 kb on - strand at 403.312 kb on - strand at 403.402 kb on + strand at 403.402 kb on + strand at 403.403 kb on - strand at 403.403 kb on - strand at 403.403 kb on - strand at 403.582 kb on - strand, within GFF2971 at 404.053 kb on - strand, within GFF2971 at 404.072 kb on + strand, within GFF2971 at 404.073 kb on - strand, within GFF2971 at 404.138 kb on - strand, within GFF2971 at 404.150 kb on + strand, within GFF2971
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 401,266 - GFF2969 0.56 -0.4 401,515 - GFF2969 0.68 +0.9 401,515 - GFF2969 0.68 +1.6 401,659 - GFF2969 0.74 +0.5 401,736 + GFF2969 0.78 +0.6 402,069 + +0.3 402,069 + +0.2 402,069 + -1.0 402,069 + -0.8 402,069 + -0.2 402,070 - +1.1 402,070 - -0.1 402,311 + GFF2970 0.10 +1.0 402,375 + GFF2970 0.17 -0.1 402,375 + GFF2970 0.17 -0.6 402,375 + GFF2970 0.17 +0.0 402,375 + GFF2970 0.17 +0.0 402,376 - GFF2970 0.17 +1.4 402,376 - GFF2970 0.17 +0.3 402,376 - GFF2970 0.17 +1.6 402,376 - GFF2970 0.17 +0.9 402,471 + GFF2970 0.27 +0.1 402,471 + GFF2970 0.27 +0.2 402,471 + GFF2970 0.27 -0.4 402,471 + GFF2970 0.27 +2.1 402,472 - GFF2970 0.27 +1.3 402,472 - GFF2970 0.27 -0.6 402,472 - GFF2970 0.27 -0.4 402,472 - GFF2970 0.27 -0.7 402,560 + GFF2970 0.36 +0.7 402,560 + GFF2970 0.36 +2.2 402,560 + GFF2970 0.36 +0.6 402,560 + GFF2970 0.36 +0.2 402,560 + GFF2970 0.36 -1.2 402,561 - GFF2970 0.36 +0.3 402,561 - GFF2970 0.36 -0.3 402,561 - GFF2970 0.36 -0.5 402,561 - GFF2970 0.36 -0.6 402,671 + GFF2970 0.47 +0.1 402,671 + GFF2970 0.47 +0.1 402,672 - GFF2970 0.47 -0.4 402,672 - GFF2970 0.47 -0.3 402,832 + GFF2970 0.63 -0.9 402,833 - GFF2970 0.63 -0.1 402,854 + GFF2970 0.65 -0.7 402,854 + GFF2970 0.65 -0.7 402,869 + GFF2970 0.67 -0.1 402,869 + GFF2970 0.67 -0.1 402,869 + GFF2970 0.67 -2.6 402,870 - GFF2970 0.67 -0.3 402,917 + GFF2970 0.72 +0.2 403,101 - +0.1 403,101 - -1.6 403,262 + -0.7 403,263 - +0.1 403,263 - -0.3 403,263 - +0.6 403,274 + +1.1 403,276 + +0.2 403,289 - +0.1 403,312 - -0.1 403,312 - -2.0 403,402 + -0.4 403,402 + -0.7 403,403 - -1.4 403,403 - +0.3 403,403 - -0.1 403,582 - GFF2971 0.27 +0.9 404,053 - GFF2971 0.75 +0.6 404,072 + GFF2971 0.77 -0.3 404,073 - GFF2971 0.77 -0.1 404,138 - GFF2971 0.84 -1.0 404,150 + GFF2971 0.85 +0.2
Or see this region's nucleotide sequence