Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF281 and GFF282 are separated by 61 nucleotides GFF282 and GFF283 overlap by 4 nucleotides
GFF281 - Outer membrane porin protein 32 precursor, at 315,865 to 316,884
GFF281
GFF282 - hypothetical protein, at 316,946 to 318,133
GFF282
GFF283 - no description, at 318,130 to 319,224
GFF283
Position (kb)
316
317
318
319 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 316.207 kb on + strand, within GFF281 at 316.208 kb on - strand, within GFF281 at 316.348 kb on + strand, within GFF281 at 316.348 kb on + strand, within GFF281 at 316.349 kb on - strand, within GFF281 at 316.379 kb on - strand, within GFF281 at 316.426 kb on + strand, within GFF281 at 316.447 kb on + strand, within GFF281 at 316.447 kb on + strand, within GFF281 at 316.447 kb on + strand, within GFF281 at 316.447 kb on + strand, within GFF281 at 316.448 kb on - strand, within GFF281 at 316.480 kb on + strand, within GFF281 at 316.480 kb on + strand, within GFF281 at 316.481 kb on - strand, within GFF281 at 316.481 kb on - strand, within GFF281 at 316.537 kb on + strand, within GFF281 at 316.537 kb on + strand, within GFF281 at 316.537 kb on + strand, within GFF281 at 316.537 kb on + strand, within GFF281 at 316.538 kb on - strand, within GFF281 at 316.538 kb on - strand, within GFF281 at 316.538 kb on - strand, within GFF281 at 316.538 kb on - strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.561 kb on + strand, within GFF281 at 316.562 kb on - strand, within GFF281 at 316.562 kb on - strand, within GFF281 at 316.562 kb on - strand, within GFF281 at 316.562 kb on - strand, within GFF281 at 316.570 kb on + strand, within GFF281 at 316.570 kb on + strand, within GFF281 at 316.571 kb on - strand, within GFF281 at 316.571 kb on - strand, within GFF281 at 316.571 kb on - strand, within GFF281 at 316.726 kb on + strand, within GFF281 at 316.726 kb on + strand, within GFF281 at 316.727 kb on - strand, within GFF281 at 316.727 kb on - strand, within GFF281 at 316.727 kb on - strand, within GFF281 at 316.759 kb on + strand, within GFF281 at 316.759 kb on + strand, within GFF281 at 316.759 kb on + strand, within GFF281 at 316.760 kb on + strand, within GFF281 at 316.760 kb on - strand, within GFF281 at 316.760 kb on - strand, within GFF281 at 316.760 kb on - strand, within GFF281 at 316.801 kb on + strand at 316.801 kb on + strand at 316.801 kb on + strand at 316.802 kb on - strand at 316.802 kb on - strand at 316.802 kb on - strand at 316.802 kb on - strand at 316.802 kb on - strand at 316.808 kb on + strand at 316.897 kb on - strand at 316.897 kb on - strand at 317.040 kb on + strand at 317.040 kb on + strand at 317.041 kb on - strand at 317.041 kb on - strand at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.220 kb on + strand, within GFF282 at 317.221 kb on - strand, within GFF282 at 317.221 kb on - strand, within GFF282 at 317.316 kb on + strand, within GFF282 at 317.317 kb on - strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.352 kb on + strand, within GFF282 at 317.353 kb on - strand, within GFF282 at 317.353 kb on - strand, within GFF282 at 317.353 kb on - strand, within GFF282 at 317.425 kb on + strand, within GFF282 at 317.613 kb on + strand, within GFF282 at 317.613 kb on + strand, within GFF282 at 317.614 kb on - strand, within GFF282 at 317.614 kb on - strand, within GFF282 at 317.673 kb on + strand, within GFF282 at 317.673 kb on + strand, within GFF282 at 317.673 kb on + strand, within GFF282 at 317.674 kb on - strand, within GFF282 at 317.697 kb on + strand, within GFF282 at 317.698 kb on - strand, within GFF282 at 317.698 kb on - strand, within GFF282 at 317.698 kb on - strand, within GFF282 at 317.698 kb on - strand, within GFF282 at 317.999 kb on - strand, within GFF282 at 317.999 kb on - strand, within GFF282 at 318.081 kb on + strand at 318.081 kb on + strand at 318.081 kb on + strand at 318.081 kb on + strand at 318.082 kb on - strand at 318.082 kb on - strand at 318.082 kb on - strand at 318.164 kb on + strand at 318.164 kb on + strand at 318.165 kb on - strand at 318.165 kb on - strand at 318.224 kb on + strand at 318.224 kb on + strand at 318.224 kb on + strand at 318.224 kb on + strand at 318.224 kb on + strand at 318.225 kb on - strand at 318.225 kb on - strand at 318.225 kb on - strand at 318.318 kb on - strand, within GFF283 at 318.642 kb on - strand, within GFF283 at 318.686 kb on + strand, within GFF283 at 318.687 kb on - strand, within GFF283 at 318.932 kb on + strand, within GFF283 at 319.064 kb on + strand, within GFF283 at 319.064 kb on + strand, within GFF283 at 319.065 kb on - strand, within GFF283
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 316,207 + GFF281 0.34 +0.2 316,208 - GFF281 0.34 -1.2 316,348 + GFF281 0.47 +0.0 316,348 + GFF281 0.47 +0.6 316,349 - GFF281 0.47 +0.8 316,379 - GFF281 0.50 -0.2 316,426 + GFF281 0.55 +1.4 316,447 + GFF281 0.57 -0.6 316,447 + GFF281 0.57 -1.4 316,447 + GFF281 0.57 -1.1 316,447 + GFF281 0.57 +0.4 316,448 - GFF281 0.57 +1.0 316,480 + GFF281 0.60 +0.0 316,480 + GFF281 0.60 +0.0 316,481 - GFF281 0.60 -0.4 316,481 - GFF281 0.60 -0.2 316,537 + GFF281 0.66 +0.5 316,537 + GFF281 0.66 -0.1 316,537 + GFF281 0.66 -0.2 316,537 + GFF281 0.66 -0.1 316,538 - GFF281 0.66 -0.4 316,538 - GFF281 0.66 +0.7 316,538 - GFF281 0.66 -1.2 316,538 - GFF281 0.66 -0.4 316,561 + GFF281 0.68 -1.3 316,561 + GFF281 0.68 -2.4 316,561 + GFF281 0.68 +2.1 316,561 + GFF281 0.68 +1.4 316,561 + GFF281 0.68 -0.4 316,562 - GFF281 0.68 -1.2 316,562 - GFF281 0.68 +0.7 316,562 - GFF281 0.68 +0.0 316,562 - GFF281 0.68 +0.2 316,570 + GFF281 0.69 +0.6 316,570 + GFF281 0.69 +1.4 316,571 - GFF281 0.69 +1.4 316,571 - GFF281 0.69 -0.7 316,571 - GFF281 0.69 -0.5 316,726 + GFF281 0.84 +1.6 316,726 + GFF281 0.84 -0.3 316,727 - GFF281 0.85 +1.0 316,727 - GFF281 0.85 -0.3 316,727 - GFF281 0.85 +0.4 316,759 + GFF281 0.88 -1.2 316,759 + GFF281 0.88 -1.3 316,759 + GFF281 0.88 +1.4 316,760 + GFF281 0.88 -0.6 316,760 - GFF281 0.88 -0.4 316,760 - GFF281 0.88 -0.2 316,760 - GFF281 0.88 -0.2 316,801 + +0.2 316,801 + -0.2 316,801 + -0.2 316,802 - -0.2 316,802 - -0.2 316,802 - -2.3 316,802 - +0.1 316,802 - -0.3 316,808 + -0.7 316,897 - +0.1 316,897 - -0.1 317,040 + -0.8 317,040 + +0.3 317,041 - -1.0 317,041 - +0.8 317,220 + GFF282 0.23 +1.5 317,220 + GFF282 0.23 -0.6 317,220 + GFF282 0.23 -0.3 317,220 + GFF282 0.23 +0.0 317,220 + GFF282 0.23 +0.6 317,220 + GFF282 0.23 +0.5 317,220 + GFF282 0.23 -0.8 317,220 + GFF282 0.23 -0.1 317,220 + GFF282 0.23 +0.7 317,220 + GFF282 0.23 -0.3 317,221 - GFF282 0.23 +0.4 317,221 - GFF282 0.23 -1.2 317,316 + GFF282 0.31 -0.1 317,317 - GFF282 0.31 +0.2 317,352 + GFF282 0.34 -1.8 317,352 + GFF282 0.34 +0.4 317,352 + GFF282 0.34 -0.1 317,352 + GFF282 0.34 +1.5 317,352 + GFF282 0.34 -0.4 317,352 + GFF282 0.34 -0.6 317,353 - GFF282 0.34 +0.4 317,353 - GFF282 0.34 +0.3 317,353 - GFF282 0.34 +1.8 317,425 + GFF282 0.40 -0.0 317,613 + GFF282 0.56 -1.0 317,613 + GFF282 0.56 +0.3 317,614 - GFF282 0.56 -0.5 317,614 - GFF282 0.56 -0.9 317,673 + GFF282 0.61 -0.1 317,673 + GFF282 0.61 -1.8 317,673 + GFF282 0.61 +0.6 317,674 - GFF282 0.61 +1.7 317,697 + GFF282 0.63 -1.5 317,698 - GFF282 0.63 +0.3 317,698 - GFF282 0.63 -0.0 317,698 - GFF282 0.63 -0.2 317,698 - GFF282 0.63 +0.0 317,999 - GFF282 0.89 +0.2 317,999 - GFF282 0.89 -0.4 318,081 + -0.2 318,081 + -1.0 318,081 + -0.2 318,081 + -0.5 318,082 - -0.9 318,082 - +0.4 318,082 - +0.7 318,164 + +0.4 318,164 + -0.5 318,165 - -0.4 318,165 - -0.8 318,224 + -0.4 318,224 + -0.7 318,224 + -0.2 318,224 + +0.2 318,224 + +0.2 318,225 - -0.1 318,225 - -1.4 318,225 - +0.0 318,318 - GFF283 0.17 -3.0 318,642 - GFF283 0.47 +1.4 318,686 + GFF283 0.51 -1.1 318,687 - GFF283 0.51 -2.8 318,932 + GFF283 0.73 +1.4 319,064 + GFF283 0.85 -0.1 319,064 + GFF283 0.85 -1.4 319,065 - GFF283 0.85 -1.2
Or see this region's nucleotide sequence