Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2659 and GFF2660 are separated by 2 nucleotides GFF2660 and GFF2661 are separated by 136 nucleotides GFF2661 and GFF2662 are separated by 39 nucleotides
GFF2659 - Electron transfer flavoprotein, alpha subunit, at 81,412 to 82,344
GFF2659
GFF2660 - Electron transfer flavoprotein, beta subunit, at 82,347 to 83,096
GFF2660
GFF2661 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 83,233 to 84,513
GFF2661
GFF2662 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 84,553 to 85,506
GFF2662
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 83.151 kb on + strand at 83.152 kb on - strand at 83.152 kb on - strand at 83.152 kb on - strand at 83.152 kb on - strand at 83.234 kb on + strand at 83.234 kb on + strand at 83.234 kb on + strand at 83.236 kb on + strand at 83.236 kb on + strand at 83.236 kb on + strand at 83.237 kb on - strand at 83.237 kb on - strand at 83.237 kb on - strand at 83.237 kb on - strand at 83.310 kb on + strand at 83.310 kb on + strand at 83.310 kb on + strand at 83.311 kb on - strand at 83.311 kb on - strand at 83.311 kb on - strand at 83.538 kb on - strand, within GFF2661 at 83.667 kb on - strand, within GFF2661 at 83.769 kb on - strand, within GFF2661 at 83.769 kb on - strand, within GFF2661 at 83.769 kb on - strand, within GFF2661 at 83.974 kb on - strand, within GFF2661 at 84.554 kb on + strand at 84.554 kb on + strand at 84.554 kb on + strand at 84.555 kb on - strand at 84.615 kb on - strand at 84.845 kb on + strand, within GFF2662 at 84.899 kb on + strand, within GFF2662 at 84.899 kb on + strand, within GFF2662 at 84.900 kb on - strand, within GFF2662 at 84.905 kb on + strand, within GFF2662 at 84.905 kb on + strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 84.906 kb on - strand, within GFF2662 at 85.490 kb on + strand at 85.490 kb on + strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand at 85.491 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 83,151 + -1.1 83,152 - -0.1 83,152 - -0.6 83,152 - -0.1 83,152 - -1.0 83,234 + -2.8 83,234 + -0.9 83,234 + +0.1 83,236 + -0.4 83,236 + -0.6 83,236 + -0.8 83,237 - +0.5 83,237 - +1.6 83,237 - -0.4 83,237 - -1.8 83,310 + -0.7 83,310 + +0.9 83,310 + -1.1 83,311 - -0.1 83,311 - +0.2 83,311 - -0.8 83,538 - GFF2661 0.24 -0.1 83,667 - GFF2661 0.34 +3.4 83,769 - GFF2661 0.42 +0.4 83,769 - GFF2661 0.42 +0.8 83,769 - GFF2661 0.42 +1.1 83,974 - GFF2661 0.58 +0.0 84,554 + -0.8 84,554 + -0.1 84,554 + +0.2 84,555 - -0.6 84,615 - -0.1 84,845 + GFF2662 0.31 +0.3 84,899 + GFF2662 0.36 +0.2 84,899 + GFF2662 0.36 +0.3 84,900 - GFF2662 0.36 +0.6 84,905 + GFF2662 0.37 -0.0 84,905 + GFF2662 0.37 -0.3 84,906 - GFF2662 0.37 -1.3 84,906 - GFF2662 0.37 -0.9 84,906 - GFF2662 0.37 +0.1 84,906 - GFF2662 0.37 -0.6 84,906 - GFF2662 0.37 +0.7 85,490 + -0.9 85,490 + -0.7 85,491 - -0.7 85,491 - -1.6 85,491 - -0.0 85,491 - -1.8 85,491 - +0.5
Or see this region's nucleotide sequence