Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2072 and GFF2073 are separated by 20 nucleotides GFF2073 and GFF2074 overlap by 4 nucleotides GFF2074 and GFF2075 are separated by 4 nucleotides
GFF2072 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 118,728 to 119,684
GFF2072
GFF2073 - Holliday junction ATP-dependent DNA helicase RuvA (EC 3.6.4.12), at 119,705 to 120,277
GFF2073
GFF2074 - hypothetical protein, at 120,274 to 121,161
GFF2074
GFF2075 - BUG/TctC family periplasmic protein, at 121,166 to 122,161
GFF2075
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 119.338 kb on + strand, within GFF2072 at 119.338 kb on + strand, within GFF2072 at 119.338 kb on + strand, within GFF2072 at 119.339 kb on - strand, within GFF2072 at 119.339 kb on - strand, within GFF2072 at 119.485 kb on + strand, within GFF2072 at 119.485 kb on + strand, within GFF2072 at 119.486 kb on - strand, within GFF2072 at 119.486 kb on - strand, within GFF2072 at 119.486 kb on - strand, within GFF2072 at 119.486 kb on - strand, within GFF2072 at 119.709 kb on + strand at 119.780 kb on + strand, within GFF2073 at 119.780 kb on + strand, within GFF2073 at 119.781 kb on - strand, within GFF2073 at 119.810 kb on + strand, within GFF2073 at 119.810 kb on + strand, within GFF2073 at 119.810 kb on + strand, within GFF2073 at 119.810 kb on + strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 120.128 kb on - strand, within GFF2073 at 120.388 kb on + strand, within GFF2074 at 120.409 kb on + strand, within GFF2074 at 120.410 kb on - strand, within GFF2074 at 120.410 kb on - strand, within GFF2074 at 121.054 kb on + strand, within GFF2074 at 121.055 kb on - strand, within GFF2074 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.587 kb on + strand, within GFF2075 at 121.587 kb on + strand, within GFF2075 at 121.588 kb on - strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.704 kb on + strand, within GFF2075 at 121.827 kb on + strand, within GFF2075 at 121.828 kb on - strand, within GFF2075 at 121.828 kb on - strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 122.061 kb on + strand, within GFF2075 at 122.061 kb on + strand, within GFF2075 at 122.062 kb on - strand at 122.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 119,338 + GFF2072 0.64 -1.0 119,338 + GFF2072 0.64 +0.2 119,338 + GFF2072 0.64 -0.6 119,339 - GFF2072 0.64 -0.2 119,339 - GFF2072 0.64 -0.7 119,485 + GFF2072 0.79 +1.6 119,485 + GFF2072 0.79 +0.8 119,486 - GFF2072 0.79 -1.2 119,486 - GFF2072 0.79 -0.3 119,486 - GFF2072 0.79 -1.3 119,486 - GFF2072 0.79 -0.4 119,709 + -1.1 119,780 + GFF2073 0.13 -0.5 119,780 + GFF2073 0.13 +0.2 119,781 - GFF2073 0.13 -2.3 119,810 + GFF2073 0.18 -1.0 119,810 + GFF2073 0.18 -1.9 119,810 + GFF2073 0.18 -0.4 119,810 + GFF2073 0.18 -0.8 119,811 - GFF2073 0.18 -0.7 119,811 - GFF2073 0.18 -2.8 119,811 - GFF2073 0.18 -0.2 119,811 - GFF2073 0.18 -0.0 119,811 - GFF2073 0.18 -0.8 119,811 - GFF2073 0.18 +0.5 120,128 - GFF2073 0.74 +0.4 120,388 + GFF2074 0.13 -0.0 120,409 + GFF2074 0.15 +0.0 120,410 - GFF2074 0.15 +0.5 120,410 - GFF2074 0.15 +0.8 121,054 + GFF2074 0.88 +2.5 121,055 - GFF2074 0.88 +0.3 121,395 + GFF2075 0.23 -2.2 121,395 + GFF2075 0.23 +0.4 121,395 + GFF2075 0.23 +0.2 121,395 + GFF2075 0.23 +0.4 121,395 + GFF2075 0.23 -0.3 121,396 - GFF2075 0.23 +0.9 121,396 - GFF2075 0.23 -0.8 121,396 - GFF2075 0.23 +0.2 121,396 - GFF2075 0.23 +0.1 121,587 + GFF2075 0.42 +0.6 121,587 + GFF2075 0.42 +1.3 121,588 - GFF2075 0.42 -0.0 121,674 + GFF2075 0.51 -0.2 121,674 + GFF2075 0.51 +0.1 121,674 + GFF2075 0.51 +0.0 121,674 + GFF2075 0.51 +0.3 121,674 + GFF2075 0.51 -0.8 121,675 - GFF2075 0.51 -0.6 121,675 - GFF2075 0.51 -1.1 121,675 - GFF2075 0.51 -1.1 121,675 - GFF2075 0.51 +0.1 121,675 - GFF2075 0.51 +0.4 121,675 - GFF2075 0.51 +0.2 121,704 + GFF2075 0.54 -1.6 121,827 + GFF2075 0.66 -0.6 121,828 - GFF2075 0.66 +0.5 121,828 - GFF2075 0.66 -1.4 121,860 + GFF2075 0.70 +1.3 121,860 + GFF2075 0.70 +0.1 121,860 + GFF2075 0.70 -0.7 121,860 + GFF2075 0.70 -0.2 121,860 + GFF2075 0.70 +0.1 122,061 + GFF2075 0.90 -0.8 122,061 + GFF2075 0.90 -0.0 122,062 - -0.2 122,062 - -1.6
Or see this region's nucleotide sequence