Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1871 and GFF1872 are separated by 36 nucleotides GFF1872 and GFF1873 are separated by 4 nucleotides GFF1873 and GFF1875 are separated by 45 nucleotides GFF1875 and GFF1874 overlap by 2 nucleotides
GFF1871 - Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.8.1), at 545,860 to 547,002
GFF1871
GFF1872 - no description, at 547,039 to 547,431
GFF1872
GFF1873 - KEGG: LacI family transcriptional regulator, at 547,436 to 548,446
GFF1873
GFF1875 - no description, at 548,492 to 548,626
GFF1875
GFF1874 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 548,625 to 549,581
GFF1874
Position (kb) 547
548
549 Strain fitness (log2 ratio) -2
-1
0
1 at 546.505 kb on + strand, within GFF1871 at 546.506 kb on - strand, within GFF1871 at 546.620 kb on + strand, within GFF1871 at 546.620 kb on + strand, within GFF1871 at 547.040 kb on + strand at 547.041 kb on - strand at 547.115 kb on + strand, within GFF1872 at 547.115 kb on + strand, within GFF1872 at 547.115 kb on + strand, within GFF1872 at 547.116 kb on - strand, within GFF1872 at 547.116 kb on - strand, within GFF1872 at 547.283 kb on + strand, within GFF1872 at 547.283 kb on + strand, within GFF1872 at 547.283 kb on + strand, within GFF1872 at 547.283 kb on + strand, within GFF1872 at 547.283 kb on + strand, within GFF1872 at 547.283 kb on + strand, within GFF1872 at 547.311 kb on - strand, within GFF1872 at 547.311 kb on - strand, within GFF1872 at 547.311 kb on - strand, within GFF1872 at 547.311 kb on - strand, within GFF1872 at 547.630 kb on - strand, within GFF1873 at 547.630 kb on - strand, within GFF1873 at 547.788 kb on + strand, within GFF1873 at 547.788 kb on + strand, within GFF1873 at 547.789 kb on - strand, within GFF1873 at 547.851 kb on + strand, within GFF1873 at 547.950 kb on + strand, within GFF1873 at 548.175 kb on + strand, within GFF1873 at 548.175 kb on + strand, within GFF1873 at 548.588 kb on - strand, within GFF1875 at 548.588 kb on - strand, within GFF1875 at 549.249 kb on + strand, within GFF1874 at 549.250 kb on - strand, within GFF1874 at 549.390 kb on + strand, within GFF1874 at 549.390 kb on + strand, within GFF1874 at 549.402 kb on + strand, within GFF1874 at 549.402 kb on + strand, within GFF1874 at 549.403 kb on - strand, within GFF1874
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 546,505 + GFF1871 0.56 -1.7 546,506 - GFF1871 0.57 -0.1 546,620 + GFF1871 0.66 +0.4 546,620 + GFF1871 0.66 +0.6 547,040 + -0.2 547,041 - -2.0 547,115 + GFF1872 0.19 -0.3 547,115 + GFF1872 0.19 -0.4 547,115 + GFF1872 0.19 +0.3 547,116 - GFF1872 0.20 -0.2 547,116 - GFF1872 0.20 +0.2 547,283 + GFF1872 0.62 -0.2 547,283 + GFF1872 0.62 +0.1 547,283 + GFF1872 0.62 -0.5 547,283 + GFF1872 0.62 -0.7 547,283 + GFF1872 0.62 -0.6 547,283 + GFF1872 0.62 +0.3 547,311 - GFF1872 0.69 +0.1 547,311 - GFF1872 0.69 +0.3 547,311 - GFF1872 0.69 +0.7 547,311 - GFF1872 0.69 -1.5 547,630 - GFF1873 0.19 -0.8 547,630 - GFF1873 0.19 +0.6 547,788 + GFF1873 0.35 +0.6 547,788 + GFF1873 0.35 -1.3 547,789 - GFF1873 0.35 -1.0 547,851 + GFF1873 0.41 -1.9 547,950 + GFF1873 0.51 -0.9 548,175 + GFF1873 0.73 +0.1 548,175 + GFF1873 0.73 -0.6 548,588 - GFF1875 0.71 -0.3 548,588 - GFF1875 0.71 -0.8 549,249 + GFF1874 0.65 -0.5 549,250 - GFF1874 0.65 +1.1 549,390 + GFF1874 0.80 -0.2 549,390 + GFF1874 0.80 +0.3 549,402 + GFF1874 0.81 -0.2 549,402 + GFF1874 0.81 -0.3 549,403 - GFF1874 0.81 -0.7
Or see this region's nucleotide sequence