Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1868 and GFF1869 are separated by 59 nucleotides GFF1869 and GFF1870 overlap by 4 nucleotides GFF1870 and GFF1871 are separated by 13 nucleotides
GFF1868 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 542,873 to 543,715
GFF1868
GFF1869 - no description, at 543,775 to 544,248
GFF1869
GFF1870 - Coenzyme A transferase, at 544,245 to 545,846
GFF1870
GFF1871 - Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.8.1), at 545,860 to 547,002
GFF1871
Position (kb) 544
545
546 Strain fitness (log2 ratio) -2
-1
0
1 at 543.253 kb on - strand, within GFF1868 at 543.253 kb on - strand, within GFF1868 at 543.333 kb on + strand, within GFF1868 at 543.333 kb on + strand, within GFF1868 at 543.334 kb on - strand, within GFF1868 at 543.334 kb on - strand, within GFF1868 at 543.357 kb on + strand, within GFF1868 at 544.010 kb on + strand, within GFF1869 at 544.011 kb on - strand, within GFF1869 at 544.223 kb on + strand at 544.223 kb on + strand at 544.786 kb on + strand, within GFF1870 at 544.787 kb on - strand, within GFF1870 at 545.293 kb on + strand, within GFF1870 at 545.452 kb on + strand, within GFF1870 at 545.452 kb on + strand, within GFF1870 at 545.453 kb on - strand, within GFF1870 at 545.453 kb on - strand, within GFF1870 at 545.924 kb on + strand at 545.924 kb on + strand at 545.925 kb on - strand at 545.925 kb on - strand at 545.925 kb on - strand at 545.925 kb on - strand at 545.981 kb on + strand, within GFF1871 at 546.218 kb on + strand, within GFF1871 at 546.218 kb on + strand, within GFF1871 at 546.505 kb on + strand, within GFF1871 at 546.506 kb on - strand, within GFF1871 at 546.620 kb on + strand, within GFF1871 at 546.620 kb on + strand, within GFF1871
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 543,253 - GFF1868 0.45 +0.1 543,253 - GFF1868 0.45 +0.4 543,333 + GFF1868 0.55 +0.6 543,333 + GFF1868 0.55 +0.4 543,334 - GFF1868 0.55 -0.4 543,334 - GFF1868 0.55 +0.1 543,357 + GFF1868 0.57 -0.3 544,010 + GFF1869 0.50 +0.2 544,011 - GFF1869 0.50 -0.5 544,223 + -0.4 544,223 + -1.0 544,786 + GFF1870 0.34 -1.2 544,787 - GFF1870 0.34 -0.4 545,293 + GFF1870 0.65 -0.4 545,452 + GFF1870 0.75 -1.2 545,452 + GFF1870 0.75 -0.6 545,453 - GFF1870 0.75 -0.2 545,453 - GFF1870 0.75 -0.4 545,924 + -2.0 545,924 + -0.4 545,925 - +0.7 545,925 - +1.2 545,925 - -0.6 545,925 - +0.7 545,981 + GFF1871 0.11 +0.2 546,218 + GFF1871 0.31 +0.0 546,218 + GFF1871 0.31 +0.6 546,505 + GFF1871 0.56 -1.7 546,506 - GFF1871 0.57 -0.1 546,620 + GFF1871 0.66 +0.4 546,620 + GFF1871 0.66 +0.6
Or see this region's nucleotide sequence