Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1769 and GFF1770 are separated by 90 nucleotides GFF1770 and GFF1771 overlap by 4 nucleotides GFF1771 and GFF1772 are separated by 292 nucleotides
GFF1769 - Urease accessory protein UreD, at 431,432 to 432,262
GFF1769
GFF1770 - cytidine/deoxycytidylate deaminase family protein, at 432,353 to 432,880
GFF1770
GFF1771 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 432,877 to 433,914
GFF1771
GFF1772 - Putative Rieske (2Fe-2S) family oxidoreductase, large subunit, at 434,207 to 435,289
GFF1772
Position (kb)
432
433
434 Strain fitness (log2 ratio)
-2
-1
0
1 at 431.926 kb on - strand, within GFF1769 at 431.926 kb on - strand, within GFF1769 at 431.926 kb on - strand, within GFF1769 at 431.926 kb on - strand, within GFF1769 at 432.175 kb on + strand, within GFF1769 at 432.176 kb on - strand, within GFF1769 at 432.231 kb on + strand at 432.232 kb on - strand at 432.232 kb on - strand at 432.232 kb on - strand at 432.273 kb on + strand at 432.273 kb on + strand at 432.273 kb on + strand at 432.274 kb on - strand at 432.274 kb on - strand at 432.274 kb on - strand at 432.274 kb on - strand at 432.274 kb on - strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.277 kb on - strand at 432.277 kb on - strand at 432.277 kb on - strand at 432.354 kb on + strand at 432.355 kb on - strand at 432.355 kb on - strand at 432.355 kb on - strand at 432.591 kb on + strand, within GFF1770 at 432.591 kb on + strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.846 kb on + strand at 432.846 kb on + strand at 433.012 kb on + strand, within GFF1771 at 433.012 kb on + strand, within GFF1771 at 433.014 kb on + strand, within GFF1771 at 433.015 kb on - strand, within GFF1771 at 433.015 kb on - strand, within GFF1771 at 433.055 kb on + strand, within GFF1771 at 433.055 kb on + strand, within GFF1771 at 433.055 kb on + strand, within GFF1771 at 433.056 kb on - strand, within GFF1771 at 433.274 kb on + strand, within GFF1771 at 433.274 kb on + strand, within GFF1771 at 433.341 kb on - strand, within GFF1771 at 433.571 kb on + strand, within GFF1771 at 433.571 kb on + strand, within GFF1771 at 433.571 kb on + strand, within GFF1771 at 433.571 kb on + strand, within GFF1771 at 433.572 kb on - strand, within GFF1771 at 433.572 kb on - strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.575 kb on - strand, within GFF1771 at 433.575 kb on - strand, within GFF1771 at 433.580 kb on + strand, within GFF1771 at 433.661 kb on + strand, within GFF1771 at 433.662 kb on - strand, within GFF1771 at 433.713 kb on - strand, within GFF1771 at 433.742 kb on + strand, within GFF1771 at 433.742 kb on + strand, within GFF1771 at 433.742 kb on + strand, within GFF1771 at 433.743 kb on - strand, within GFF1771 at 433.743 kb on - strand, within GFF1771 at 433.743 kb on - strand, within GFF1771 at 433.743 kb on - strand, within GFF1771 at 433.746 kb on - strand, within GFF1771 at 433.746 kb on - strand, within GFF1771 at 433.746 kb on - strand, within GFF1771 at 434.028 kb on + strand at 434.028 kb on + strand at 434.028 kb on + strand at 434.028 kb on + strand at 434.029 kb on - strand at 434.029 kb on - strand at 434.116 kb on + strand at 434.116 kb on + strand at 434.116 kb on + strand at 434.116 kb on + strand at 434.116 kb on + strand at 434.117 kb on - strand at 434.117 kb on - strand at 434.117 kb on - strand at 434.465 kb on + strand, within GFF1772 at 434.466 kb on - strand, within GFF1772 at 434.466 kb on - strand, within GFF1772 at 434.491 kb on + strand, within GFF1772 at 434.491 kb on + strand, within GFF1772 at 434.502 kb on - strand, within GFF1772 at 434.546 kb on + strand, within GFF1772 at 434.546 kb on + strand, within GFF1772 at 434.547 kb on - strand, within GFF1772 at 434.570 kb on + strand, within GFF1772 at 434.570 kb on + strand, within GFF1772 at 434.570 kb on + strand, within GFF1772 at 434.570 kb on + strand, within GFF1772 at 434.571 kb on - strand, within GFF1772 at 434.571 kb on - strand, within GFF1772 at 434.571 kb on - strand, within GFF1772 at 434.660 kb on + strand, within GFF1772 at 434.660 kb on + strand, within GFF1772 at 434.777 kb on + strand, within GFF1772 at 434.777 kb on + strand, within GFF1772 at 434.777 kb on + strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.903 kb on + strand, within GFF1772 at 434.903 kb on + strand, within GFF1772 at 434.903 kb on + strand, within GFF1772 at 434.903 kb on + strand, within GFF1772
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 431,926 - GFF1769 0.59 -0.6 431,926 - GFF1769 0.59 -0.4 431,926 - GFF1769 0.59 +0.9 431,926 - GFF1769 0.59 +0.0 432,175 + GFF1769 0.89 +0.1 432,176 - GFF1769 0.90 -1.2 432,231 + -0.3 432,232 - -0.1 432,232 - -1.0 432,232 - -0.1 432,273 + -1.2 432,273 + +0.2 432,273 + -0.1 432,274 - +0.0 432,274 - -0.4 432,274 - +0.0 432,274 - -0.9 432,274 - -1.2 432,276 + -0.8 432,276 + -0.1 432,276 + -0.5 432,276 + -0.3 432,276 + +0.4 432,276 + +0.5 432,277 - +0.5 432,277 - -0.0 432,277 - -0.1 432,354 + -0.6 432,355 - +0.6 432,355 - +0.8 432,355 - +0.9 432,591 + GFF1770 0.45 -1.1 432,591 + GFF1770 0.45 -2.2 432,592 - GFF1770 0.45 -0.6 432,592 - GFF1770 0.45 -1.0 432,592 - GFF1770 0.45 +0.2 432,592 - GFF1770 0.45 -0.9 432,592 - GFF1770 0.45 -1.8 432,846 + -1.0 432,846 + -1.2 433,012 + GFF1771 0.13 +0.8 433,012 + GFF1771 0.13 +0.2 433,014 + GFF1771 0.13 -0.6 433,015 - GFF1771 0.13 -1.5 433,015 - GFF1771 0.13 -1.9 433,055 + GFF1771 0.17 -0.5 433,055 + GFF1771 0.17 -1.8 433,055 + GFF1771 0.17 +0.4 433,056 - GFF1771 0.17 -0.6 433,274 + GFF1771 0.38 -0.4 433,274 + GFF1771 0.38 -0.3 433,341 - GFF1771 0.45 -0.1 433,571 + GFF1771 0.67 -1.2 433,571 + GFF1771 0.67 +1.7 433,571 + GFF1771 0.67 -0.2 433,571 + GFF1771 0.67 -0.5 433,572 - GFF1771 0.67 -0.3 433,572 - GFF1771 0.67 +0.9 433,574 + GFF1771 0.67 -0.1 433,574 + GFF1771 0.67 -1.5 433,574 + GFF1771 0.67 -0.4 433,574 + GFF1771 0.67 -0.2 433,574 + GFF1771 0.67 -0.9 433,575 - GFF1771 0.67 -1.8 433,575 - GFF1771 0.67 -1.1 433,580 + GFF1771 0.68 -1.5 433,661 + GFF1771 0.76 +0.1 433,662 - GFF1771 0.76 +1.1 433,713 - GFF1771 0.81 -0.0 433,742 + GFF1771 0.83 -0.5 433,742 + GFF1771 0.83 +1.4 433,742 + GFF1771 0.83 -0.8 433,743 - GFF1771 0.83 -0.3 433,743 - GFF1771 0.83 -0.2 433,743 - GFF1771 0.83 +0.0 433,743 - GFF1771 0.83 -0.9 433,746 - GFF1771 0.84 +0.9 433,746 - GFF1771 0.84 -0.3 433,746 - GFF1771 0.84 +1.7 434,028 + +0.7 434,028 + -1.1 434,028 + -2.4 434,028 + -0.3 434,029 - +0.0 434,029 - -0.4 434,116 + -0.0 434,116 + -0.0 434,116 + +0.2 434,116 + +0.7 434,116 + +0.2 434,117 - -0.9 434,117 - +0.7 434,117 - +0.4 434,465 + GFF1772 0.24 +0.5 434,466 - GFF1772 0.24 -0.3 434,466 - GFF1772 0.24 +0.5 434,491 + GFF1772 0.26 -0.3 434,491 + GFF1772 0.26 -0.6 434,502 - GFF1772 0.27 +0.1 434,546 + GFF1772 0.31 +0.1 434,546 + GFF1772 0.31 -0.8 434,547 - GFF1772 0.31 +1.0 434,570 + GFF1772 0.34 -0.4 434,570 + GFF1772 0.34 +0.5 434,570 + GFF1772 0.34 +0.5 434,570 + GFF1772 0.34 -1.3 434,571 - GFF1772 0.34 -1.1 434,571 - GFF1772 0.34 +0.2 434,571 - GFF1772 0.34 +0.0 434,660 + GFF1772 0.42 -0.5 434,660 + GFF1772 0.42 +0.6 434,777 + GFF1772 0.53 -0.4 434,777 + GFF1772 0.53 +0.1 434,777 + GFF1772 0.53 -0.4 434,778 - GFF1772 0.53 -1.7 434,778 - GFF1772 0.53 -0.5 434,778 - GFF1772 0.53 -0.6 434,778 - GFF1772 0.53 +1.0 434,778 - GFF1772 0.53 +0.4 434,778 - GFF1772 0.53 +0.7 434,778 - GFF1772 0.53 -0.6 434,903 + GFF1772 0.64 -0.3 434,903 + GFF1772 0.64 -0.6 434,903 + GFF1772 0.64 -0.3 434,903 + GFF1772 0.64 +0.2
Or see this region's nucleotide sequence