Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1500 and GFF1501 are separated by 128 nucleotides GFF1501 and GFF1502 are separated by 281 nucleotides GFF1502 and GFF1503 are separated by 113 nucleotides GFF1503 and GFF1504 are separated by 71 nucleotides
GFF1500 - Transcriptional regulator, IclR family, at 92,874 to 93,683
GFF1500
GFF1501 - hypothetical protein, at 93,812 to 94,066
GFF1501
GFF1502 - no description, at 94,348 to 94,464
GFF1502
GFF1503 - Dihydrofolate reductase homolog, at 94,578 to 95,210
GFF1503
GFF1504 - Alkylhydroperoxidase AhpD domain protein, at 95,282 to 96,454
GFF1504
Position (kb)
94
95
96 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 93.621 kb on + strand at 93.622 kb on - strand at 93.681 kb on + strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.727 kb on - strand at 93.727 kb on - strand at 93.840 kb on + strand, within GFF1501 at 93.874 kb on + strand, within GFF1501 at 93.874 kb on - strand, within GFF1501 at 93.942 kb on + strand, within GFF1501 at 93.942 kb on + strand, within GFF1501 at 93.942 kb on + strand, within GFF1501 at 93.943 kb on - strand, within GFF1501 at 94.034 kb on - strand, within GFF1501 at 94.124 kb on + strand at 94.124 kb on + strand at 94.125 kb on - strand at 94.125 kb on - strand at 94.125 kb on - strand at 94.196 kb on + strand at 94.196 kb on + strand at 94.196 kb on + strand at 94.197 kb on - strand at 94.197 kb on - strand at 94.282 kb on + strand at 94.283 kb on - strand at 94.528 kb on + strand at 94.528 kb on + strand at 94.531 kb on - strand at 94.531 kb on - strand at 94.532 kb on + strand at 94.585 kb on - strand at 94.588 kb on + strand at 94.620 kb on + strand at 94.620 kb on + strand at 94.620 kb on + strand at 94.620 kb on + strand at 94.620 kb on + strand at 94.620 kb on + strand at 94.621 kb on - strand at 94.621 kb on - strand at 94.621 kb on - strand at 94.621 kb on - strand at 94.621 kb on - strand at 94.659 kb on + strand, within GFF1503 at 94.659 kb on + strand, within GFF1503 at 94.659 kb on - strand, within GFF1503 at 94.716 kb on + strand, within GFF1503 at 94.717 kb on - strand, within GFF1503 at 94.884 kb on + strand, within GFF1503 at 94.884 kb on + strand, within GFF1503 at 94.885 kb on - strand, within GFF1503 at 94.885 kb on - strand, within GFF1503 at 94.885 kb on - strand, within GFF1503 at 94.885 kb on - strand, within GFF1503 at 94.885 kb on - strand, within GFF1503 at 94.885 kb on - strand, within GFF1503 at 94.885 kb on - strand, within GFF1503 at 94.941 kb on + strand, within GFF1503 at 94.942 kb on - strand, within GFF1503 at 95.043 kb on + strand, within GFF1503 at 95.043 kb on + strand, within GFF1503 at 95.044 kb on - strand, within GFF1503 at 95.196 kb on + strand at 95.197 kb on - strand at 95.197 kb on - strand at 95.584 kb on - strand, within GFF1504 at 95.658 kb on + strand, within GFF1504 at 95.658 kb on + strand, within GFF1504 at 95.658 kb on + strand, within GFF1504 at 95.658 kb on + strand, within GFF1504 at 95.658 kb on + strand, within GFF1504 at 95.659 kb on - strand, within GFF1504 at 95.659 kb on - strand, within GFF1504 at 95.659 kb on - strand, within GFF1504 at 95.659 kb on - strand, within GFF1504 at 95.659 kb on - strand, within GFF1504 at 95.659 kb on - strand, within GFF1504 at 95.659 kb on - strand, within GFF1504 at 95.724 kb on + strand, within GFF1504 at 95.724 kb on + strand, within GFF1504 at 95.724 kb on + strand, within GFF1504 at 95.724 kb on + strand, within GFF1504 at 95.724 kb on + strand, within GFF1504 at 95.725 kb on - strand, within GFF1504 at 95.725 kb on - strand, within GFF1504 at 95.725 kb on - strand, within GFF1504 at 95.725 kb on - strand, within GFF1504 at 95.725 kb on - strand, within GFF1504 at 95.727 kb on + strand, within GFF1504 at 95.728 kb on - strand, within GFF1504 at 95.728 kb on - strand, within GFF1504 at 95.728 kb on - strand, within GFF1504 at 95.728 kb on - strand, within GFF1504 at 95.728 kb on - strand, within GFF1504 at 95.844 kb on + strand, within GFF1504 at 95.844 kb on + strand, within GFF1504 at 95.844 kb on + strand, within GFF1504 at 95.845 kb on - strand, within GFF1504
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 93,621 + -0.3 93,622 - +1.4 93,681 + -0.4 93,682 - +0.7 93,682 - -0.4 93,682 - +0.4 93,682 - +0.1 93,682 - -0.9 93,682 - -0.4 93,727 - -0.4 93,727 - -0.6 93,840 + GFF1501 0.11 -0.4 93,874 + GFF1501 0.24 -0.5 93,874 - GFF1501 0.24 -0.3 93,942 + GFF1501 0.51 +0.5 93,942 + GFF1501 0.51 -0.5 93,942 + GFF1501 0.51 +0.1 93,943 - GFF1501 0.51 -0.6 94,034 - GFF1501 0.87 -0.6 94,124 + +0.4 94,124 + +0.5 94,125 - -0.4 94,125 - +1.3 94,125 - +0.7 94,196 + +0.1 94,196 + -0.5 94,196 + +1.3 94,197 - -1.8 94,197 - -0.5 94,282 + +0.7 94,283 - +0.0 94,528 + -0.8 94,528 + +1.4 94,531 - +0.4 94,531 - +0.7 94,532 + +0.7 94,585 - +0.1 94,588 + -1.6 94,620 + -0.2 94,620 + -1.1 94,620 + -0.2 94,620 + -0.7 94,620 + +0.0 94,620 + -0.8 94,621 - +0.7 94,621 - -2.3 94,621 - +0.1 94,621 - +0.4 94,621 - +0.1 94,659 + GFF1503 0.13 -0.6 94,659 + GFF1503 0.13 -0.9 94,659 - GFF1503 0.13 -0.8 94,716 + GFF1503 0.22 +0.1 94,717 - GFF1503 0.22 -0.2 94,884 + GFF1503 0.48 -0.5 94,884 + GFF1503 0.48 +0.4 94,885 - GFF1503 0.48 -0.5 94,885 - GFF1503 0.48 -0.9 94,885 - GFF1503 0.48 -0.0 94,885 - GFF1503 0.48 -1.0 94,885 - GFF1503 0.48 -1.1 94,885 - GFF1503 0.48 +0.8 94,885 - GFF1503 0.48 -0.6 94,941 + GFF1503 0.57 +0.7 94,942 - GFF1503 0.58 -0.1 95,043 + GFF1503 0.73 -0.7 95,043 + GFF1503 0.73 -0.0 95,044 - GFF1503 0.74 -0.6 95,196 + +0.1 95,197 - -0.3 95,197 - -3.0 95,584 - GFF1504 0.26 -0.9 95,658 + GFF1504 0.32 -1.8 95,658 + GFF1504 0.32 +1.1 95,658 + GFF1504 0.32 -1.4 95,658 + GFF1504 0.32 +0.4 95,658 + GFF1504 0.32 -0.4 95,659 - GFF1504 0.32 -0.3 95,659 - GFF1504 0.32 -0.7 95,659 - GFF1504 0.32 -0.6 95,659 - GFF1504 0.32 -0.9 95,659 - GFF1504 0.32 -0.3 95,659 - GFF1504 0.32 -1.6 95,659 - GFF1504 0.32 +0.5 95,724 + GFF1504 0.38 +0.7 95,724 + GFF1504 0.38 -0.1 95,724 + GFF1504 0.38 -0.3 95,724 + GFF1504 0.38 -1.3 95,724 + GFF1504 0.38 -1.3 95,725 - GFF1504 0.38 -0.6 95,725 - GFF1504 0.38 +0.4 95,725 - GFF1504 0.38 +0.5 95,725 - GFF1504 0.38 -0.3 95,725 - GFF1504 0.38 +0.9 95,727 + GFF1504 0.38 -0.4 95,728 - GFF1504 0.38 -0.9 95,728 - GFF1504 0.38 +0.3 95,728 - GFF1504 0.38 +2.0 95,728 - GFF1504 0.38 -0.4 95,728 - GFF1504 0.38 -0.6 95,844 + GFF1504 0.48 -2.0 95,844 + GFF1504 0.48 -0.2 95,844 + GFF1504 0.48 -0.8 95,845 - GFF1504 0.48 +0.4
Or see this region's nucleotide sequence