Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1422 and GFF1423 overlap by 8 nucleotides GFF1423 and GFF1424 overlap by 4 nucleotides GFF1424 and GFF1425 overlap by 1 nucleotides
GFF1422 - L-lactate dehydrogenase, at 13,271 to 14,431
GFF1422
GFF1423 - Phenylacetate ABC transporter, permease protein 1, at 14,424 to 15,296
GFF1423
GFF1424 - Phenylacetate ABC transporter, permease protein 2, at 15,293 to 16,225
GFF1424
GFF1425 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 16,225 to 16,974
GFF1425
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.830 kb on + strand, within GFF1422 at 14.399 kb on + strand at 14.508 kb on + strand at 14.508 kb on + strand at 14.508 kb on + strand at 14.509 kb on - strand at 15.063 kb on + strand, within GFF1423 at 15.189 kb on + strand, within GFF1423 at 15.190 kb on - strand, within GFF1423 at 15.204 kb on + strand, within GFF1423 at 15.292 kb on + strand at 15.292 kb on + strand at 15.293 kb on - strand at 15.764 kb on + strand, within GFF1424 at 15.764 kb on + strand, within GFF1424 at 15.765 kb on - strand, within GFF1424 at 15.765 kb on - strand, within GFF1424 at 15.765 kb on - strand, within GFF1424 at 15.818 kb on + strand, within GFF1424 at 15.818 kb on + strand, within GFF1424 at 15.818 kb on + strand, within GFF1424 at 15.818 kb on + strand, within GFF1424 at 15.968 kb on + strand, within GFF1424 at 15.968 kb on + strand, within GFF1424 at 15.969 kb on - strand, within GFF1424 at 15.969 kb on - strand, within GFF1424 at 16.125 kb on - strand, within GFF1424 at 16.125 kb on - strand, within GFF1424 at 16.235 kb on + strand at 16.236 kb on - strand at 16.236 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 13,830 + GFF1422 0.48 +0.2 14,399 + -1.8 14,508 + -0.8 14,508 + +2.3 14,508 + +0.3 14,509 - -0.5 15,063 + GFF1423 0.73 -0.6 15,189 + GFF1423 0.88 -1.0 15,190 - GFF1423 0.88 -0.3 15,204 + GFF1423 0.89 -2.6 15,292 + +0.1 15,292 + -0.2 15,293 - -0.1 15,764 + GFF1424 0.50 -0.8 15,764 + GFF1424 0.50 -0.6 15,765 - GFF1424 0.51 -1.5 15,765 - GFF1424 0.51 -0.4 15,765 - GFF1424 0.51 -0.1 15,818 + GFF1424 0.56 +1.0 15,818 + GFF1424 0.56 -0.9 15,818 + GFF1424 0.56 +0.9 15,818 + GFF1424 0.56 +2.0 15,968 + GFF1424 0.72 -0.1 15,968 + GFF1424 0.72 +0.5 15,969 - GFF1424 0.72 -0.5 15,969 - GFF1424 0.72 -0.1 16,125 - GFF1424 0.89 -1.0 16,125 - GFF1424 0.89 -0.2 16,235 + -1.2 16,236 - -0.7 16,236 - -1.2
Or see this region's nucleotide sequence