Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1232 and GFF1233 are separated by 31 nucleotides GFF1233 and GFF1234 overlap by 4 nucleotides GFF1234 and GFF1235 are separated by 102 nucleotides GFF1235 and GFF1236 are separated by 104 nucleotides
GFF1232 - Uncharacterized protein conserved in bacteria, at 552,414 to 553,199
GFF1232
GFF1233 - Arginine/ornithine antiporter ArcD, at 553,231 to 553,875
GFF1233
GFF1234 - Peptide-methionine (S)-S-oxide reductase MsrA (EC 1.8.4.11), at 553,872 to 554,573
GFF1234
GFF1235 - Methionine sulfoxide reductase-associated methionine-rich protein, at 554,676 to 554,942
GFF1235
GFF1236 - Methionine sulfoxide reductase molybdopterin-binding subunit, at 555,047 to 555,838
GFF1236
Position (kb) 553
554
555 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 552.924 kb on + strand, within GFF1232 at 552.925 kb on - strand, within GFF1232 at 552.925 kb on - strand, within GFF1232 at 552.925 kb on - strand, within GFF1232 at 552.925 kb on - strand, within GFF1232 at 552.925 kb on - strand, within GFF1232 at 552.925 kb on - strand, within GFF1232 at 552.981 kb on + strand, within GFF1232 at 552.981 kb on + strand, within GFF1232 at 552.981 kb on + strand, within GFF1232 at 552.982 kb on - strand, within GFF1232 at 552.982 kb on - strand, within GFF1232 at 553.010 kb on + strand, within GFF1232 at 553.010 kb on + strand, within GFF1232 at 553.010 kb on + strand, within GFF1232 at 553.010 kb on + strand, within GFF1232 at 553.010 kb on + strand, within GFF1232 at 553.010 kb on + strand, within GFF1232 at 553.011 kb on - strand, within GFF1232 at 553.011 kb on - strand, within GFF1232 at 553.011 kb on - strand, within GFF1232 at 553.014 kb on + strand, within GFF1232 at 553.014 kb on + strand, within GFF1232 at 553.014 kb on + strand, within GFF1232 at 553.014 kb on + strand, within GFF1232 at 553.014 kb on + strand, within GFF1232 at 553.014 kb on + strand, within GFF1232 at 553.015 kb on - strand, within GFF1232 at 553.056 kb on + strand, within GFF1232 at 553.057 kb on - strand, within GFF1232 at 553.380 kb on - strand, within GFF1233 at 553.915 kb on + strand at 553.915 kb on + strand at 553.916 kb on - strand at 553.916 kb on - strand at 553.916 kb on - strand at 553.916 kb on - strand at 553.916 kb on - strand at 553.975 kb on + strand, within GFF1234 at 553.976 kb on - strand, within GFF1234 at 553.976 kb on - strand, within GFF1234 at 554.002 kb on + strand, within GFF1234 at 554.003 kb on - strand, within GFF1234 at 554.003 kb on - strand, within GFF1234 at 554.003 kb on - strand, within GFF1234 at 554.015 kb on - strand, within GFF1234 at 554.075 kb on - strand, within GFF1234 at 554.075 kb on - strand, within GFF1234 at 554.104 kb on + strand, within GFF1234 at 554.104 kb on + strand, within GFF1234 at 554.104 kb on + strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.105 kb on - strand, within GFF1234 at 554.158 kb on + strand, within GFF1234 at 554.158 kb on + strand, within GFF1234 at 554.221 kb on + strand, within GFF1234 at 554.222 kb on - strand, within GFF1234 at 554.242 kb on + strand, within GFF1234 at 554.263 kb on + strand, within GFF1234 at 554.264 kb on - strand, within GFF1234 at 554.264 kb on - strand, within GFF1234 at 554.264 kb on - strand, within GFF1234 at 554.347 kb on + strand, within GFF1234 at 554.518 kb on + strand at 554.677 kb on + strand at 554.677 kb on + strand at 554.678 kb on - strand at 554.678 kb on - strand at 554.678 kb on - strand at 555.069 kb on + strand at 555.069 kb on + strand at 555.088 kb on - strand at 555.099 kb on + strand at 555.099 kb on + strand at 555.100 kb on - strand at 555.144 kb on + strand, within GFF1236 at 555.145 kb on - strand, within GFF1236 at 555.145 kb on - strand, within GFF1236 at 555.186 kb on + strand, within GFF1236 at 555.186 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.270 kb on + strand, within GFF1236 at 555.271 kb on - strand, within GFF1236 at 555.271 kb on - strand, within GFF1236 at 555.271 kb on - strand, within GFF1236 at 555.273 kb on + strand, within GFF1236 at 555.273 kb on + strand, within GFF1236 at 555.273 kb on + strand, within GFF1236 at 555.274 kb on - strand, within GFF1236 at 555.274 kb on - strand, within GFF1236 at 555.300 kb on + strand, within GFF1236 at 555.300 kb on + strand, within GFF1236 at 555.300 kb on + strand, within GFF1236 at 555.300 kb on + strand, within GFF1236 at 555.301 kb on - strand, within GFF1236 at 555.301 kb on - strand, within GFF1236 at 555.301 kb on - strand, within GFF1236 at 555.301 kb on - strand, within GFF1236 at 555.489 kb on + strand, within GFF1236 at 555.489 kb on + strand, within GFF1236 at 555.489 kb on + strand, within GFF1236 at 555.490 kb on - strand, within GFF1236 at 555.528 kb on + strand, within GFF1236 at 555.528 kb on + strand, within GFF1236 at 555.528 kb on + strand, within GFF1236 at 555.529 kb on - strand, within GFF1236 at 555.529 kb on - strand, within GFF1236 at 555.531 kb on + strand, within GFF1236 at 555.532 kb on - strand, within GFF1236 at 555.573 kb on + strand, within GFF1236
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 552,924 + GFF1232 0.65 +0.2 552,925 - GFF1232 0.65 -0.1 552,925 - GFF1232 0.65 -0.2 552,925 - GFF1232 0.65 +0.2 552,925 - GFF1232 0.65 -0.5 552,925 - GFF1232 0.65 +0.0 552,925 - GFF1232 0.65 -0.7 552,981 + GFF1232 0.72 +0.2 552,981 + GFF1232 0.72 +1.4 552,981 + GFF1232 0.72 -0.0 552,982 - GFF1232 0.72 -1.2 552,982 - GFF1232 0.72 -0.0 553,010 + GFF1232 0.76 +0.3 553,010 + GFF1232 0.76 -0.2 553,010 + GFF1232 0.76 -0.4 553,010 + GFF1232 0.76 -0.3 553,010 + GFF1232 0.76 -0.1 553,010 + GFF1232 0.76 -0.2 553,011 - GFF1232 0.76 -0.0 553,011 - GFF1232 0.76 +1.8 553,011 - GFF1232 0.76 -1.3 553,014 + GFF1232 0.76 -0.7 553,014 + GFF1232 0.76 -2.8 553,014 + GFF1232 0.76 +0.8 553,014 + GFF1232 0.76 -1.2 553,014 + GFF1232 0.76 -1.4 553,014 + GFF1232 0.76 -0.2 553,015 - GFF1232 0.76 +1.2 553,056 + GFF1232 0.82 +0.2 553,057 - GFF1232 0.82 -0.1 553,380 - GFF1233 0.23 -0.2 553,915 + -0.2 553,915 + +0.1 553,916 - -0.6 553,916 - -0.9 553,916 - -0.3 553,916 - -0.9 553,916 - +0.1 553,975 + GFF1234 0.15 -0.4 553,976 - GFF1234 0.15 +1.1 553,976 - GFF1234 0.15 -0.3 554,002 + GFF1234 0.19 -0.3 554,003 - GFF1234 0.19 -0.2 554,003 - GFF1234 0.19 -0.2 554,003 - GFF1234 0.19 +2.0 554,015 - GFF1234 0.20 +0.6 554,075 - GFF1234 0.29 -1.6 554,075 - GFF1234 0.29 -0.6 554,104 + GFF1234 0.33 -1.0 554,104 + GFF1234 0.33 -0.2 554,104 + GFF1234 0.33 +0.3 554,105 - GFF1234 0.33 +0.1 554,105 - GFF1234 0.33 -0.3 554,105 - GFF1234 0.33 +1.8 554,105 - GFF1234 0.33 -0.0 554,105 - GFF1234 0.33 -0.8 554,158 + GFF1234 0.41 -0.2 554,158 + GFF1234 0.41 -0.1 554,221 + GFF1234 0.50 -0.0 554,222 - GFF1234 0.50 -0.2 554,242 + GFF1234 0.53 -0.5 554,263 + GFF1234 0.56 -0.2 554,264 - GFF1234 0.56 +0.9 554,264 - GFF1234 0.56 +1.1 554,264 - GFF1234 0.56 -0.3 554,347 + GFF1234 0.68 -0.3 554,518 + +0.1 554,677 + -0.1 554,677 + +1.4 554,678 - -0.2 554,678 - +0.2 554,678 - -1.4 555,069 + +0.4 555,069 + +0.1 555,088 - -1.8 555,099 + -0.2 555,099 + -0.2 555,100 - +0.7 555,144 + GFF1236 0.12 +0.0 555,145 - GFF1236 0.12 -0.9 555,145 - GFF1236 0.12 +0.5 555,186 + GFF1236 0.18 -0.5 555,186 + GFF1236 0.18 -2.2 555,270 + GFF1236 0.28 -0.6 555,270 + GFF1236 0.28 -0.4 555,270 + GFF1236 0.28 +0.4 555,270 + GFF1236 0.28 -0.5 555,270 + GFF1236 0.28 +0.2 555,270 + GFF1236 0.28 -0.8 555,271 - GFF1236 0.28 +1.4 555,271 - GFF1236 0.28 -2.3 555,271 - GFF1236 0.28 +0.1 555,273 + GFF1236 0.29 +0.3 555,273 + GFF1236 0.29 -0.6 555,273 + GFF1236 0.29 +1.1 555,274 - GFF1236 0.29 +0.5 555,274 - GFF1236 0.29 +1.7 555,300 + GFF1236 0.32 +0.5 555,300 + GFF1236 0.32 +1.2 555,300 + GFF1236 0.32 -0.1 555,300 + GFF1236 0.32 -0.3 555,301 - GFF1236 0.32 -1.0 555,301 - GFF1236 0.32 +0.4 555,301 - GFF1236 0.32 +0.4 555,301 - GFF1236 0.32 -0.0 555,489 + GFF1236 0.56 -0.6 555,489 + GFF1236 0.56 +0.8 555,489 + GFF1236 0.56 -0.6 555,490 - GFF1236 0.56 -0.2 555,528 + GFF1236 0.61 -0.7 555,528 + GFF1236 0.61 +0.0 555,528 + GFF1236 0.61 +0.0 555,529 - GFF1236 0.61 -0.2 555,529 - GFF1236 0.61 +0.8 555,531 + GFF1236 0.61 -0.4 555,532 - GFF1236 0.61 -0.3 555,573 + GFF1236 0.66 -0.8
Or see this region's nucleotide sequence