Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF115 and GFF116 are separated by 3 nucleotides GFF116 and GFF117 are separated by 188 nucleotides GFF117 and GFF118 are separated by 13 nucleotides
GFF115 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 143,390 to 144,055
GFF115
GFF116 - RidA/YER057c/UK114 superfamily, group 1, at 144,059 to 144,571
GFF116
GFF117 - no description, at 144,760 to 145,539
GFF117
GFF118 - putative membrane protein, at 145,553 to 146,578
GFF118
Position (kb)
144
145
146 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 143.827 kb on - strand, within GFF115 at 143.827 kb on - strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.877 kb on + strand, within GFF115 at 143.878 kb on - strand, within GFF115 at 143.878 kb on - strand, within GFF115 at 143.878 kb on - strand, within GFF115 at 143.878 kb on - strand, within GFF115 at 143.977 kb on - strand, within GFF115 at 143.977 kb on - strand, within GFF115 at 144.019 kb on - strand at 144.048 kb on + strand at 144.048 kb on + strand at 144.333 kb on + strand, within GFF116 at 144.333 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.417 kb on + strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.418 kb on - strand, within GFF116 at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.685 kb on + strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.686 kb on - strand at 144.712 kb on - strand at 144.872 kb on - strand, within GFF117 at 145.120 kb on + strand, within GFF117 at 145.120 kb on + strand, within GFF117 at 145.121 kb on - strand, within GFF117 at 145.121 kb on - strand, within GFF117 at 145.121 kb on - strand, within GFF117 at 145.273 kb on + strand, within GFF117 at 145.273 kb on + strand, within GFF117 at 145.274 kb on - strand, within GFF117 at 145.274 kb on - strand, within GFF117 at 145.274 kb on - strand, within GFF117 at 145.367 kb on - strand, within GFF117 at 145.367 kb on - strand, within GFF117 at 145.498 kb on + strand at 145.499 kb on - strand at 145.499 kb on - strand at 145.499 kb on - strand at 145.531 kb on + strand at 145.532 kb on - strand at 145.532 kb on - strand at 145.643 kb on + strand at 145.644 kb on - strand at 145.674 kb on + strand, within GFF118 at 145.674 kb on + strand, within GFF118 at 145.675 kb on - strand, within GFF118 at 145.675 kb on - strand, within GFF118 at 145.675 kb on - strand, within GFF118 at 145.675 kb on - strand, within GFF118 at 145.675 kb on - strand, within GFF118 at 145.761 kb on - strand, within GFF118 at 145.926 kb on - strand, within GFF118 at 145.953 kb on - strand, within GFF118 at 145.995 kb on - strand, within GFF118 at 145.995 kb on - strand, within GFF118 at 145.995 kb on - strand, within GFF118 at 146.204 kb on + strand, within GFF118 at 146.204 kb on + strand, within GFF118 at 146.204 kb on + strand, within GFF118 at 146.204 kb on + strand, within GFF118 at 146.204 kb on + strand, within GFF118 at 146.205 kb on - strand, within GFF118 at 146.205 kb on - strand, within GFF118 at 146.205 kb on - strand, within GFF118 at 146.210 kb on + strand, within GFF118 at 146.210 kb on + strand, within GFF118 at 146.210 kb on + strand, within GFF118 at 146.211 kb on - strand, within GFF118 at 146.211 kb on - strand, within GFF118 at 146.300 kb on + strand, within GFF118 at 146.400 kb on - strand, within GFF118 at 146.411 kb on + strand, within GFF118 at 146.411 kb on + strand, within GFF118 at 146.418 kb on - strand, within GFF118
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 143,827 - GFF115 0.66 +0.8 143,827 - GFF115 0.66 +0.4 143,877 + GFF115 0.73 +0.1 143,877 + GFF115 0.73 -1.0 143,877 + GFF115 0.73 +1.8 143,877 + GFF115 0.73 -1.0 143,877 + GFF115 0.73 -1.1 143,877 + GFF115 0.73 -0.2 143,877 + GFF115 0.73 +0.4 143,878 - GFF115 0.73 -0.8 143,878 - GFF115 0.73 -0.1 143,878 - GFF115 0.73 -0.2 143,878 - GFF115 0.73 -1.2 143,977 - GFF115 0.88 -1.0 143,977 - GFF115 0.88 -0.3 144,019 - -0.9 144,048 + +0.4 144,048 + +0.1 144,333 + GFF116 0.53 +0.1 144,333 + GFF116 0.53 +0.0 144,417 + GFF116 0.70 -0.6 144,417 + GFF116 0.70 +0.4 144,417 + GFF116 0.70 +0.4 144,417 + GFF116 0.70 -1.7 144,417 + GFF116 0.70 +0.9 144,418 - GFF116 0.70 -0.4 144,418 - GFF116 0.70 -1.2 144,418 - GFF116 0.70 -0.1 144,418 - GFF116 0.70 -0.6 144,418 - GFF116 0.70 +0.3 144,685 + -2.7 144,685 + -0.2 144,685 + -0.2 144,685 + +0.4 144,685 + -0.6 144,685 + -0.2 144,685 + -0.1 144,686 - +0.3 144,686 - +0.1 144,686 - -0.1 144,686 - -0.4 144,686 - -0.9 144,686 - -0.2 144,686 - -0.2 144,686 - -0.3 144,712 - -0.2 144,872 - GFF117 0.14 -0.2 145,120 + GFF117 0.46 -1.6 145,120 + GFF117 0.46 +0.4 145,121 - GFF117 0.46 -0.7 145,121 - GFF117 0.46 -1.6 145,121 - GFF117 0.46 +0.4 145,273 + GFF117 0.66 -0.6 145,273 + GFF117 0.66 -1.3 145,274 - GFF117 0.66 -2.1 145,274 - GFF117 0.66 +0.0 145,274 - GFF117 0.66 +0.4 145,367 - GFF117 0.78 -0.8 145,367 - GFF117 0.78 +0.6 145,498 + -0.7 145,499 - +0.6 145,499 - -1.0 145,499 - +0.0 145,531 + +0.8 145,532 - +0.1 145,532 - +0.7 145,643 + +1.5 145,644 - -1.1 145,674 + GFF118 0.12 -0.9 145,674 + GFF118 0.12 +1.0 145,675 - GFF118 0.12 -0.6 145,675 - GFF118 0.12 -1.2 145,675 - GFF118 0.12 +0.9 145,675 - GFF118 0.12 -1.0 145,675 - GFF118 0.12 -2.9 145,761 - GFF118 0.20 -0.9 145,926 - GFF118 0.36 -1.2 145,953 - GFF118 0.39 -0.8 145,995 - GFF118 0.43 +0.9 145,995 - GFF118 0.43 -0.5 145,995 - GFF118 0.43 -1.7 146,204 + GFF118 0.63 +0.4 146,204 + GFF118 0.63 -0.3 146,204 + GFF118 0.63 -0.6 146,204 + GFF118 0.63 -0.6 146,204 + GFF118 0.63 -0.5 146,205 - GFF118 0.64 +0.9 146,205 - GFF118 0.64 -1.6 146,205 - GFF118 0.64 +0.0 146,210 + GFF118 0.64 -0.6 146,210 + GFF118 0.64 +0.4 146,210 + GFF118 0.64 -1.2 146,211 - GFF118 0.64 +0.4 146,211 - GFF118 0.64 +0.0 146,300 + GFF118 0.73 +0.5 146,400 - GFF118 0.83 -0.1 146,411 + GFF118 0.84 -0.3 146,411 + GFF118 0.84 -0.5 146,418 - GFF118 0.84 -1.0
Or see this region's nucleotide sequence