Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7337 and GFF7338 overlap by 8 nucleotides GFF7338 and GFF7339 overlap by 4 nucleotides GFF7339 and GFF7340 are separated by 4 nucleotides
GFF7337 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 6,175 to 7,137
GFF7337
GFF7338 - ABC transporter, permease protein (cluster 10, nitrate/sulfonate/bicarbonate), at 7,130 to 7,957
GFF7338
GFF7339 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 7,954 to 8,781
GFF7339
GFF7340 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 8,786 to 9,877
GFF7340
Position (kb) 7
8 Strain fitness (log2 ratio) -2
-1
0
1
2 at 6.300 kb on - strand, within GFF7337 at 6.381 kb on - strand, within GFF7337 at 6.537 kb on - strand, within GFF7337 at 6.638 kb on + strand, within GFF7337 at 6.639 kb on - strand, within GFF7337 at 7.239 kb on + strand, within GFF7338 at 7.342 kb on - strand, within GFF7338 at 7.947 kb on - strand at 8.174 kb on + strand, within GFF7339 at 8.175 kb on - strand, within GFF7339 at 8.742 kb on - strand at 8.847 kb on + strand at 8.864 kb on + strand at 8.864 kb on + strand at 8.910 kb on + strand, within GFF7340 at 8.911 kb on - strand, within GFF7340
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 6,300 - GFF7337 0.13 -1.5 6,381 - GFF7337 0.21 +0.1 6,537 - GFF7337 0.38 -0.6 6,638 + GFF7337 0.48 +0.5 6,639 - GFF7337 0.48 +0.5 7,239 + GFF7338 0.13 +1.0 7,342 - GFF7338 0.26 +0.1 7,947 - -0.8 8,174 + GFF7339 0.27 +1.0 8,175 - GFF7339 0.27 +1.1 8,742 - -0.9 8,847 + -0.8 8,864 + -2.6 8,864 + +1.8 8,910 + GFF7340 0.11 -0.2 8,911 - GFF7340 0.11 -0.5
Or see this region's nucleotide sequence