Strain Fitness in Variovorax sp. SCN45 around GFF5730

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5729 and GFF5730 are separated by 20 nucleotidesGFF5730 and GFF5731 are separated by 67 nucleotides GFF5729 - Methyl-accepting chemotaxis sensor/transducer protein, at 99,299 to 100,903 GFF5729 GFF5730 - Positive regulator of CheA protein activity (CheW), at 100,924 to 101,445 GFF5730 GFF5731 - Methyl-accepting chemotaxis sensor/transducer protein, at 101,513 to 103,114 GFF5731 Position (kb) 100 101 102Strain fitness (log2 ratio) -2 -1 0 1at 101.690 kb on + strand, within GFF5731at 101.691 kb on - strand, within GFF5731at 101.691 kb on - strand, within GFF5731at 101.888 kb on + strand, within GFF5731at 101.990 kb on + strand, within GFF5731at 101.990 kb on + strand, within GFF5731

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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101,690 + GFF5731 0.11 +0.1
101,691 - GFF5731 0.11 +1.2
101,691 - GFF5731 0.11 -1.2
101,888 + GFF5731 0.23 +1.5
101,990 + GFF5731 0.30 -2.3
101,990 + GFF5731 0.30 -0.6

Or see this region's nucleotide sequence