Strain Fitness in Variovorax sp. SCN45 around GFF5115

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5114 and GFF5115 are separated by 17 nucleotidesGFF5115 and GFF5116 overlap by 1 nucleotides GFF5114 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 214,865 to 216,097 GFF5114 GFF5115 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 216,115 to 217,233 GFF5115 GFF5116 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 217,233 to 218,735 GFF5116 Position (kb) 216 217 218Strain fitness (log2 ratio) -1 0 1 2at 215.298 kb on + strand, within GFF5114at 215.299 kb on - strand, within GFF5114at 215.299 kb on - strand, within GFF5114at 215.299 kb on - strand, within GFF5114at 215.299 kb on - strand, within GFF5114at 215.383 kb on - strand, within GFF5114at 215.397 kb on + strand, within GFF5114at 215.397 kb on + strand, within GFF5114at 215.397 kb on + strand, within GFF5114at 215.398 kb on - strand, within GFF5114at 215.826 kb on + strand, within GFF5114at 215.899 kb on + strand, within GFF5114at 215.900 kb on - strand, within GFF5114at 215.900 kb on - strand, within GFF5114at 215.900 kb on - strand, within GFF5114at 215.940 kb on + strand, within GFF5114at 215.940 kb on + strand, within GFF5114at 215.940 kb on + strand, within GFF5114at 215.940 kb on + strand, within GFF5114at 215.940 kb on + strand, within GFF5114at 215.940 kb on + strand, within GFF5114at 215.940 kb on + strand, within GFF5114at 215.941 kb on - strand, within GFF5114at 215.941 kb on - strand, within GFF5114at 215.941 kb on - strand, within GFF5114at 215.941 kb on - strand, within GFF5114at 215.941 kb on - strand, within GFF5114at 215.941 kb on - strand, within GFF5114at 215.941 kb on - strand, within GFF5114at 215.985 kb on + strandat 215.985 kb on + strandat 215.985 kb on + strandat 215.985 kb on + strandat 215.986 kb on - strandat 215.986 kb on - strandat 215.986 kb on - strandat 215.986 kb on - strandat 215.986 kb on - strandat 216.116 kb on + strandat 216.117 kb on - strandat 216.117 kb on - strandat 216.332 kb on + strand, within GFF5115at 216.332 kb on + strand, within GFF5115at 216.332 kb on + strand, within GFF5115at 216.332 kb on + strand, within GFF5115at 216.333 kb on - strand, within GFF5115at 216.575 kb on + strand, within GFF5115at 216.576 kb on - strand, within GFF5115at 216.576 kb on - strand, within GFF5115at 216.576 kb on - strand, within GFF5115at 216.576 kb on - strand, within GFF5115at 216.597 kb on - strand, within GFF5115at 216.597 kb on - strand, within GFF5115at 216.597 kb on - strand, within GFF5115at 216.597 kb on - strand, within GFF5115at 216.869 kb on + strand, within GFF5115at 216.869 kb on + strand, within GFF5115at 216.870 kb on - strand, within GFF5115at 216.870 kb on - strand, within GFF5115at 216.870 kb on - strand, within GFF5115at 217.064 kb on + strand, within GFF5115at 217.064 kb on + strand, within GFF5115at 217.065 kb on - strand, within GFF5115at 217.065 kb on - strand, within GFF5115at 217.124 kb on + strandat 217.125 kb on - strandat 217.198 kb on + strandat 217.199 kb on - strandat 217.199 kb on - strandat 217.199 kb on - strandat 217.604 kb on - strand, within GFF5116at 217.681 kb on + strand, within GFF5116at 217.681 kb on + strand, within GFF5116at 217.681 kb on + strand, within GFF5116at 217.681 kb on + strand, within GFF5116at 217.681 kb on + strand, within GFF5116at 217.681 kb on + strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.682 kb on - strand, within GFF5116at 217.885 kb on - strand, within GFF5116at 217.886 kb on - strand, within GFF5116

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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215,298 + GFF5114 0.35 -0.5
215,299 - GFF5114 0.35 -1.2
215,299 - GFF5114 0.35 +0.4
215,299 - GFF5114 0.35 +0.2
215,299 - GFF5114 0.35 -0.8
215,383 - GFF5114 0.42 +0.3
215,397 + GFF5114 0.43 -0.6
215,397 + GFF5114 0.43 -0.7
215,397 + GFF5114 0.43 +2.2
215,398 - GFF5114 0.43 -1.0
215,826 + GFF5114 0.78 -1.6
215,899 + GFF5114 0.84 +0.2
215,900 - GFF5114 0.84 -0.6
215,900 - GFF5114 0.84 +1.1
215,900 - GFF5114 0.84 +0.2
215,940 + GFF5114 0.87 -1.5
215,940 + GFF5114 0.87 -0.3
215,940 + GFF5114 0.87 -0.3
215,940 + GFF5114 0.87 -0.2
215,940 + GFF5114 0.87 -0.6
215,940 + GFF5114 0.87 -0.6
215,940 + GFF5114 0.87 +0.8
215,941 - GFF5114 0.87 +1.2
215,941 - GFF5114 0.87 -0.7
215,941 - GFF5114 0.87 +2.2
215,941 - GFF5114 0.87 +0.6
215,941 - GFF5114 0.87 -1.3
215,941 - GFF5114 0.87 -0.2
215,941 - GFF5114 0.87 -0.3
215,985 + +0.6
215,985 + -0.4
215,985 + +0.2
215,985 + +0.2
215,986 - +1.0
215,986 - +2.2
215,986 - +0.0
215,986 - -0.7
215,986 - -0.4
216,116 + -1.7
216,117 - -1.3
216,117 - +0.2
216,332 + GFF5115 0.19 -1.0
216,332 + GFF5115 0.19 +0.1
216,332 + GFF5115 0.19 +0.1
216,332 + GFF5115 0.19 +0.6
216,333 - GFF5115 0.19 +0.1
216,575 + GFF5115 0.41 -0.5
216,576 - GFF5115 0.41 -0.2
216,576 - GFF5115 0.41 +0.4
216,576 - GFF5115 0.41 -1.1
216,576 - GFF5115 0.41 -0.4
216,597 - GFF5115 0.43 -1.2
216,597 - GFF5115 0.43 -0.3
216,597 - GFF5115 0.43 -0.0
216,597 - GFF5115 0.43 +0.4
216,869 + GFF5115 0.67 -0.2
216,869 + GFF5115 0.67 -0.3
216,870 - GFF5115 0.67 -1.1
216,870 - GFF5115 0.67 +0.1
216,870 - GFF5115 0.67 -1.3
217,064 + GFF5115 0.85 -1.5
217,064 + GFF5115 0.85 -1.4
217,065 - GFF5115 0.85 +0.5
217,065 - GFF5115 0.85 +0.5
217,124 + -0.1
217,125 - -0.7
217,198 + +2.0
217,199 - -1.7
217,199 - +0.4
217,199 - +0.3
217,604 - GFF5116 0.25 +0.3
217,681 + GFF5116 0.30 -0.2
217,681 + GFF5116 0.30 -0.3
217,681 + GFF5116 0.30 -0.3
217,681 + GFF5116 0.30 -0.5
217,681 + GFF5116 0.30 -0.5
217,681 + GFF5116 0.30 -0.6
217,682 - GFF5116 0.30 -0.4
217,682 - GFF5116 0.30 +0.4
217,682 - GFF5116 0.30 -0.4
217,682 - GFF5116 0.30 -1.4
217,682 - GFF5116 0.30 -1.0
217,682 - GFF5116 0.30 -0.6
217,682 - GFF5116 0.30 -0.8
217,682 - GFF5116 0.30 +0.0
217,682 - GFF5116 0.30 -0.8
217,682 - GFF5116 0.30 +0.5
217,682 - GFF5116 0.30 +1.0
217,682 - GFF5116 0.30 -0.0
217,682 - GFF5116 0.30 -0.5
217,682 - GFF5116 0.30 -0.1
217,682 - GFF5116 0.30 -0.1
217,682 - GFF5116 0.30 +0.1
217,885 - GFF5116 0.43 +0.1
217,886 - GFF5116 0.43 -1.0

Or see this region's nucleotide sequence