Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4846 and GFF4847 are separated by 19 nucleotides GFF4847 and GFF4848 overlap by 4 nucleotides GFF4848 and GFF4849 overlap by 4 nucleotides GFF4849 and GFF4850 overlap by 4 nucleotides GFF4850 and GFF4851 overlap by 4 nucleotides
GFF4846 - Conjugative transfer protein TrbE, at 215,637 to 218,114
GFF4846
GFF4847 - Conjugative transfer protein TrbD, at 218,134 to 218,418
GFF4847
GFF4848 - Conjugative transfer protein TrbC, at 218,415 to 218,807
GFF4848
GFF4849 - Conjugative transfer protein TrbB, at 218,804 to 219,841
GFF4849
GFF4850 - CopG domain-containing protein, at 219,838 to 220,302
GFF4850
GFF4851 - Coupling protein VirD4, ATPase required for T-DNA transfer, at 220,299 to 222,317
GFF4851
Position (kb) 218
219
220 Strain fitness (log2 ratio) -2
-1
0
1
2 at 217.943 kb on + strand at 217.943 kb on + strand at 217.943 kb on + strand at 217.943 kb on + strand at 217.943 kb on + strand at 217.943 kb on + strand at 217.944 kb on - strand at 217.944 kb on - strand at 217.944 kb on - strand at 217.944 kb on - strand at 218.021 kb on + strand at 218.021 kb on + strand at 218.022 kb on - strand at 218.022 kb on - strand at 218.022 kb on - strand at 218.119 kb on + strand at 218.119 kb on + strand at 218.120 kb on - strand at 218.135 kb on + strand at 218.136 kb on - strand at 218.136 kb on - strand at 218.163 kb on + strand, within GFF4847 at 218.164 kb on - strand, within GFF4847 at 218.419 kb on - strand at 218.419 kb on - strand at 218.777 kb on + strand at 218.902 kb on - strand at 218.902 kb on - strand at 219.119 kb on + strand, within GFF4849 at 219.119 kb on + strand, within GFF4849 at 219.119 kb on + strand, within GFF4849 at 219.120 kb on - strand, within GFF4849 at 219.245 kb on + strand, within GFF4849 at 219.245 kb on + strand, within GFF4849 at 219.246 kb on - strand, within GFF4849 at 219.453 kb on + strand, within GFF4849 at 219.841 kb on + strand at 219.841 kb on + strand at 219.842 kb on - strand at 219.842 kb on - strand at 219.842 kb on - strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.448 kb on - strand at 220.448 kb on - strand at 220.448 kb on - strand at 220.448 kb on - strand at 220.681 kb on + strand, within GFF4851 at 220.681 kb on + strand, within GFF4851 at 220.682 kb on - strand, within GFF4851 at 220.682 kb on - strand, within GFF4851 at 220.684 kb on + strand, within GFF4851 at 220.685 kb on - strand, within GFF4851 at 220.758 kb on + strand, within GFF4851 at 220.758 kb on + strand, within GFF4851 at 220.759 kb on - strand, within GFF4851 at 220.759 kb on - strand, within GFF4851 at 220.759 kb on - strand, within GFF4851
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 217,943 + -0.3 217,943 + -0.6 217,943 + -1.1 217,943 + +0.3 217,943 + +0.3 217,943 + +0.2 217,944 - -0.2 217,944 - -0.1 217,944 - -1.2 217,944 - -0.4 218,021 + -0.3 218,021 + +0.1 218,022 - -0.1 218,022 - +0.1 218,022 - -0.3 218,119 + -0.5 218,119 + -0.1 218,120 - -0.1 218,135 + -0.1 218,136 - -0.6 218,136 - -2.0 218,163 + GFF4847 0.10 +0.6 218,164 - GFF4847 0.11 -0.3 218,419 - -1.3 218,419 - -0.5 218,777 + +0.2 218,902 - +0.2 218,902 - +0.1 219,119 + GFF4849 0.30 +0.2 219,119 + GFF4849 0.30 +0.1 219,119 + GFF4849 0.30 +0.2 219,120 - GFF4849 0.30 -0.1 219,245 + GFF4849 0.42 +0.1 219,245 + GFF4849 0.42 +0.3 219,246 - GFF4849 0.43 -0.4 219,453 + GFF4849 0.63 +0.1 219,841 + +0.7 219,841 + -0.1 219,842 - -0.0 219,842 - +1.0 219,842 - +1.5 220,302 + -1.6 220,302 + +0.3 220,302 + -0.3 220,302 + -2.2 220,302 + +0.1 220,302 + -0.4 220,302 + +0.0 220,303 - +2.0 220,303 - +0.9 220,303 - -0.0 220,303 - -0.8 220,303 - +0.3 220,303 - +0.6 220,447 + -0.8 220,447 + +1.1 220,447 + -1.3 220,447 + -0.2 220,447 + +1.2 220,447 + -0.4 220,447 + +0.2 220,448 - +0.7 220,448 - +0.3 220,448 - +0.2 220,448 - +0.8 220,681 + GFF4851 0.19 +0.1 220,681 + GFF4851 0.19 +1.0 220,682 - GFF4851 0.19 -0.1 220,682 - GFF4851 0.19 -0.6 220,684 + GFF4851 0.19 +0.5 220,685 - GFF4851 0.19 +0.1 220,758 + GFF4851 0.23 +0.1 220,758 + GFF4851 0.23 +0.6 220,759 - GFF4851 0.23 -0.7 220,759 - GFF4851 0.23 +0.5 220,759 - GFF4851 0.23 +0.0
Or see this region's nucleotide sequence