Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4454 and GFF4455 are separated by 44 nucleotides GFF4455 and GFF4456 are separated by 16 nucleotides GFF4456 and GFF4457 overlap by 4 nucleotides GFF4457 and GFF4458 overlap by 35 nucleotides
GFF4454 - Exoribonuclease II (EC 3.1.13.1), at 94,625 to 96,682
GFF4454
GFF4455 - TonB protein, at 96,727 to 97,581
GFF4455
GFF4456 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 97,598 to 98,470
GFF4456
GFF4457 - Monofunctional biosynthetic peptidoglycan transglycosylase, at 98,467 to 99,210
GFF4457
GFF4458 - hypothetical protein, at 99,176 to 99,580
GFF4458
Position (kb) 97
98
99 Strain fitness (log2 ratio) -2
-1
0
1
2 at 96.721 kb on + strand at 96.721 kb on + strand at 96.721 kb on + strand at 97.231 kb on + strand, within GFF4455 at 97.231 kb on + strand, within GFF4455 at 97.231 kb on + strand, within GFF4455 at 97.231 kb on + strand, within GFF4455 at 97.232 kb on - strand, within GFF4455 at 97.270 kb on + strand, within GFF4455 at 97.271 kb on - strand, within GFF4455 at 97.271 kb on - strand, within GFF4455 at 97.271 kb on - strand, within GFF4455 at 97.345 kb on + strand, within GFF4455 at 97.345 kb on + strand, within GFF4455 at 97.346 kb on - strand, within GFF4455 at 97.346 kb on - strand, within GFF4455 at 97.346 kb on - strand, within GFF4455 at 97.607 kb on + strand at 97.607 kb on + strand at 97.607 kb on + strand at 97.608 kb on - strand at 97.608 kb on - strand at 97.608 kb on - strand at 97.608 kb on - strand at 97.694 kb on + strand, within GFF4456 at 97.695 kb on - strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.277 kb on - strand, within GFF4456 at 98.277 kb on - strand, within GFF4456 at 98.277 kb on - strand, within GFF4456 at 98.277 kb on - strand, within GFF4456 at 98.656 kb on + strand, within GFF4457 at 98.656 kb on + strand, within GFF4457 at 98.990 kb on - strand, within GFF4457 at 99.046 kb on + strand, within GFF4457 at 99.046 kb on + strand, within GFF4457 at 99.046 kb on + strand, within GFF4457 at 99.047 kb on - strand, within GFF4457 at 99.049 kb on + strand, within GFF4457 at 99.050 kb on - strand, within GFF4457 at 99.120 kb on + strand, within GFF4457 at 99.151 kb on + strand at 99.261 kb on + strand, within GFF4458 at 99.261 kb on + strand, within GFF4458 at 99.261 kb on + strand, within GFF4458 at 99.262 kb on - strand, within GFF4458 at 99.262 kb on - strand, within GFF4458 at 99.351 kb on + strand, within GFF4458 at 99.351 kb on + strand, within GFF4458 at 99.351 kb on + strand, within GFF4458 at 99.352 kb on - strand, within GFF4458 at 99.352 kb on - strand, within GFF4458 at 99.441 kb on + strand, within GFF4458 at 99.441 kb on + strand, within GFF4458 at 99.441 kb on + strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 96,721 + -0.8 96,721 + +0.7 96,721 + -0.6 97,231 + GFF4455 0.59 +0.7 97,231 + GFF4455 0.59 +0.3 97,231 + GFF4455 0.59 +0.6 97,231 + GFF4455 0.59 -0.1 97,232 - GFF4455 0.59 -0.2 97,270 + GFF4455 0.64 -0.6 97,271 - GFF4455 0.64 +1.1 97,271 - GFF4455 0.64 +0.3 97,271 - GFF4455 0.64 -0.2 97,345 + GFF4455 0.72 +0.4 97,345 + GFF4455 0.72 +0.1 97,346 - GFF4455 0.72 -0.2 97,346 - GFF4455 0.72 -0.3 97,346 - GFF4455 0.72 -0.4 97,607 + +0.1 97,607 + -2.0 97,607 + +1.1 97,608 - -1.2 97,608 - -1.8 97,608 - +0.0 97,608 - -0.3 97,694 + GFF4456 0.11 +0.1 97,695 - GFF4456 0.11 -0.8 98,276 + GFF4456 0.78 -0.5 98,276 + GFF4456 0.78 -1.0 98,276 + GFF4456 0.78 -1.3 98,276 + GFF4456 0.78 -0.6 98,276 + GFF4456 0.78 -1.0 98,276 + GFF4456 0.78 -0.4 98,276 + GFF4456 0.78 -0.6 98,276 + GFF4456 0.78 -0.2 98,277 - GFF4456 0.78 -1.4 98,277 - GFF4456 0.78 -1.1 98,277 - GFF4456 0.78 -1.1 98,277 - GFF4456 0.78 -1.1 98,656 + GFF4457 0.25 -1.1 98,656 + GFF4457 0.25 -1.6 98,990 - GFF4457 0.70 -0.2 99,046 + GFF4457 0.78 -0.7 99,046 + GFF4457 0.78 -0.7 99,046 + GFF4457 0.78 +0.1 99,047 - GFF4457 0.78 -0.5 99,049 + GFF4457 0.78 -0.2 99,050 - GFF4457 0.78 -0.3 99,120 + GFF4457 0.88 +0.1 99,151 + -1.1 99,261 + GFF4458 0.21 -1.6 99,261 + GFF4458 0.21 +0.3 99,261 + GFF4458 0.21 -0.2 99,262 - GFF4458 0.21 -0.9 99,262 - GFF4458 0.21 +0.6 99,351 + GFF4458 0.43 +0.9 99,351 + GFF4458 0.43 -0.7 99,351 + GFF4458 0.43 -0.5 99,352 - GFF4458 0.43 -1.0 99,352 - GFF4458 0.43 -0.1 99,441 + GFF4458 0.65 +0.1 99,441 + GFF4458 0.65 +0.3 99,441 + GFF4458 0.65 -0.7 99,442 - GFF4458 0.66 +2.1 99,442 - GFF4458 0.66 -0.6 99,442 - GFF4458 0.66 -2.0 99,442 - GFF4458 0.66 -0.3 99,442 - GFF4458 0.66 +0.3 99,442 - GFF4458 0.66 +0.2 99,442 - GFF4458 0.66 -0.2 99,442 - GFF4458 0.66 +0.3
Or see this region's nucleotide sequence