Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4231 and GFF4232 are separated by 76 nucleotides GFF4232 and GFF4233 overlap by 4 nucleotides GFF4233 and GFF4234 overlap by 4 nucleotides GFF4234 and GFF4235 overlap by 4 nucleotides
GFF4231 - no description, at 143,055 to 144,026
GFF4231
GFF4232 - no description, at 144,103 to 144,657
GFF4232
GFF4233 - no description, at 144,654 to 145,064
GFF4233
GFF4234 - hypothetical protein, at 145,061 to 145,852
GFF4234
GFF4235 - Hydroxymethylpyrimidine ABC transporter, ATPase component, at 145,849 to 146,667
GFF4235
Position (kb) 144
145
146 Strain fitness (log2 ratio) -1
0
1
2
3 at 143.774 kb on - strand, within GFF4231 at 143.774 kb on - strand, within GFF4231 at 144.033 kb on - strand at 144.304 kb on + strand, within GFF4232 at 144.304 kb on + strand, within GFF4232 at 144.305 kb on - strand, within GFF4232 at 144.511 kb on + strand, within GFF4232 at 144.511 kb on + strand, within GFF4232 at 144.512 kb on - strand, within GFF4232 at 144.512 kb on - strand, within GFF4232 at 144.720 kb on + strand, within GFF4233 at 144.721 kb on - strand, within GFF4233 at 144.786 kb on + strand, within GFF4233 at 144.817 kb on - strand, within GFF4233 at 145.074 kb on + strand at 145.075 kb on - strand at 145.906 kb on + strand at 145.906 kb on + strand at 145.907 kb on - strand at 145.907 kb on - strand at 145.907 kb on - strand at 145.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 143,774 - GFF4231 0.74 -0.3 143,774 - GFF4231 0.74 +3.0 144,033 - -1.2 144,304 + GFF4232 0.36 -0.5 144,304 + GFF4232 0.36 -0.4 144,305 - GFF4232 0.36 -1.3 144,511 + GFF4232 0.74 -1.0 144,511 + GFF4232 0.74 +0.5 144,512 - GFF4232 0.74 +1.3 144,512 - GFF4232 0.74 -1.4 144,720 + GFF4233 0.16 -1.0 144,721 - GFF4233 0.16 -0.4 144,786 + GFF4233 0.32 -0.8 144,817 - GFF4233 0.40 -1.0 145,074 + +0.2 145,075 - -1.0 145,906 + -0.7 145,906 + -0.5 145,907 - +0.1 145,907 - -0.4 145,907 - +1.2 145,907 - -0.5
Or see this region's nucleotide sequence