Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3066 and GFF3067 are separated by 27 nucleotides GFF3067 and GFF3068 are separated by 28 nucleotides GFF3068 and GFF3069 are separated by 140 nucleotides
GFF3066 - Nitrogen regulation protein NR(I), GlnG (=NtrC), at 499,383 to 500,918
GFF3066
GFF3067 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 500,946 to 502,025
GFF3067
GFF3068 - FIG00538108: hypothetical protein, at 502,054 to 502,563
GFF3068
GFF3069 - Glutamine synthetase type I (EC 6.3.1.2), at 502,704 to 504,119
GFF3069
Position (kb) 500
501
502
503 Strain fitness (log2 ratio) -2
-1
0
1
2 at 500.128 kb on + strand, within GFF3066 at 500.605 kb on + strand, within GFF3066 at 500.898 kb on + strand at 500.901 kb on + strand at 500.901 kb on + strand at 500.902 kb on - strand at 500.902 kb on - strand at 500.959 kb on - strand at 500.964 kb on + strand at 500.964 kb on + strand at 500.964 kb on + strand at 500.964 kb on + strand at 500.964 kb on + strand at 500.964 kb on + strand at 500.965 kb on - strand at 500.965 kb on - strand at 500.965 kb on - strand at 500.965 kb on - strand at 500.965 kb on - strand at 500.965 kb on - strand at 501.078 kb on + strand, within GFF3067 at 501.089 kb on - strand, within GFF3067 at 501.214 kb on - strand, within GFF3067 at 501.508 kb on + strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.509 kb on - strand, within GFF3067 at 501.779 kb on - strand, within GFF3067 at 501.779 kb on - strand, within GFF3067 at 502.025 kb on + strand at 502.025 kb on + strand at 502.026 kb on - strand at 502.026 kb on - strand at 502.048 kb on + strand at 502.048 kb on + strand at 502.048 kb on + strand at 502.048 kb on + strand at 502.049 kb on - strand at 502.157 kb on + strand, within GFF3068 at 502.157 kb on + strand, within GFF3068 at 502.157 kb on + strand, within GFF3068 at 502.157 kb on + strand, within GFF3068 at 502.157 kb on + strand, within GFF3068 at 502.158 kb on - strand, within GFF3068 at 502.158 kb on - strand, within GFF3068 at 502.166 kb on + strand, within GFF3068 at 502.211 kb on + strand, within GFF3068 at 502.211 kb on + strand, within GFF3068 at 502.211 kb on + strand, within GFF3068 at 502.212 kb on - strand, within GFF3068 at 502.212 kb on - strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.469 kb on + strand, within GFF3068 at 502.470 kb on - strand, within GFF3068 at 502.470 kb on - strand, within GFF3068 at 502.472 kb on + strand, within GFF3068 at 502.568 kb on - strand at 502.568 kb on - strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.673 kb on + strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand at 502.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 500,128 + GFF3066 0.49 -1.0 500,605 + GFF3066 0.80 -1.4 500,898 + -0.9 500,901 + +0.6 500,901 + -0.1 500,902 - -0.6 500,902 - -0.4 500,959 - +0.2 500,964 + -0.5 500,964 + -1.1 500,964 + -0.5 500,964 + +0.3 500,964 + -0.9 500,964 + +0.9 500,965 - +0.8 500,965 - -0.2 500,965 - -0.7 500,965 - -0.1 500,965 - +0.0 500,965 - -0.1 501,078 + GFF3067 0.12 +0.5 501,089 - GFF3067 0.13 +0.1 501,214 - GFF3067 0.25 -0.6 501,508 + GFF3067 0.52 -0.1 501,509 - GFF3067 0.52 +0.6 501,509 - GFF3067 0.52 -0.2 501,509 - GFF3067 0.52 -1.1 501,509 - GFF3067 0.52 +2.3 501,509 - GFF3067 0.52 +0.2 501,779 - GFF3067 0.77 +1.2 501,779 - GFF3067 0.77 -0.6 502,025 + -0.1 502,025 + -0.1 502,026 - +0.6 502,026 - -1.0 502,048 + -0.3 502,048 + +0.1 502,048 + -1.9 502,048 + -1.1 502,049 - -0.3 502,157 + GFF3068 0.20 +0.2 502,157 + GFF3068 0.20 +1.1 502,157 + GFF3068 0.20 -1.3 502,157 + GFF3068 0.20 -2.0 502,157 + GFF3068 0.20 -0.5 502,158 - GFF3068 0.20 -0.7 502,158 - GFF3068 0.20 -0.4 502,166 + GFF3068 0.22 -0.8 502,211 + GFF3068 0.31 -0.9 502,211 + GFF3068 0.31 -0.4 502,211 + GFF3068 0.31 -1.1 502,212 - GFF3068 0.31 -1.2 502,212 - GFF3068 0.31 +0.9 502,469 + GFF3068 0.81 -0.9 502,469 + GFF3068 0.81 -0.1 502,469 + GFF3068 0.81 -0.5 502,469 + GFF3068 0.81 -0.2 502,469 + GFF3068 0.81 -0.1 502,470 - GFF3068 0.82 -0.8 502,470 - GFF3068 0.82 -1.7 502,472 + GFF3068 0.82 -1.2 502,568 - -0.0 502,568 - -1.3 502,673 + -0.5 502,673 + -1.1 502,673 + -1.2 502,673 + +0.4 502,673 + +0.2 502,673 + -0.8 502,673 + -0.5 502,673 + -0.6 502,673 + -0.1 502,673 + -0.3 502,673 + +0.1 502,673 + +0.6 502,673 + -0.5 502,673 + +0.9 502,673 + +1.2 502,674 - +1.5 502,674 - -1.5 502,674 - -0.7 502,674 - -0.1 502,674 - +0.5 502,674 - -0.2 502,674 - -1.1
Or see this region's nucleotide sequence