Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1017 and GFF1018 are separated by 33 nucleotides GFF1018 and GFF1019 are separated by 7 nucleotides
GFF1017 - conserved hypothetical protein-transmembrane prediction, at 334,966 to 335,946
GFF1017
GFF1018 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase (EC 2.1.1.185), at 335,980 to 336,726
GFF1018
GFF1019 - monooxygenase, FAD-binding, at 336,734 to 337,759
GFF1019
Position (kb)
335
336
337 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 335.161 kb on + strand, within GFF1017 at 335.161 kb on + strand, within GFF1017 at 335.162 kb on - strand, within GFF1017 at 335.162 kb on - strand, within GFF1017 at 335.162 kb on - strand, within GFF1017 at 335.240 kb on - strand, within GFF1017 at 335.333 kb on + strand, within GFF1017 at 335.545 kb on + strand, within GFF1017 at 335.545 kb on + strand, within GFF1017 at 335.546 kb on - strand, within GFF1017 at 335.546 kb on - strand, within GFF1017 at 335.546 kb on - strand, within GFF1017 at 335.650 kb on + strand, within GFF1017 at 335.650 kb on + strand, within GFF1017 at 335.650 kb on + strand, within GFF1017 at 335.650 kb on + strand, within GFF1017 at 335.650 kb on + strand, within GFF1017 at 335.651 kb on - strand, within GFF1017 at 335.651 kb on - strand, within GFF1017 at 335.651 kb on - strand, within GFF1017 at 335.651 kb on - strand, within GFF1017 at 335.651 kb on - strand, within GFF1017 at 335.651 kb on - strand, within GFF1017 at 335.651 kb on - strand, within GFF1017 at 335.891 kb on + strand at 335.892 kb on - strand at 335.965 kb on - strand at 335.965 kb on - strand at 335.965 kb on - strand at 335.970 kb on + strand at 335.971 kb on - strand at 336.042 kb on + strand at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.442 kb on + strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.443 kb on - strand, within GFF1018 at 336.534 kb on + strand, within GFF1018 at 336.534 kb on + strand, within GFF1018 at 336.534 kb on + strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.535 kb on - strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.538 kb on + strand, within GFF1018 at 336.539 kb on - strand, within GFF1018 at 336.539 kb on - strand, within GFF1018 at 336.697 kb on + strand at 336.697 kb on + strand at 336.697 kb on + strand at 336.698 kb on - strand at 336.698 kb on - strand at 336.735 kb on + strand at 336.735 kb on + strand at 336.735 kb on + strand at 336.736 kb on - strand at 336.736 kb on - strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.777 kb on + strand at 336.778 kb on - strand at 336.778 kb on - strand at 336.778 kb on - strand at 336.778 kb on - strand at 336.894 kb on + strand, within GFF1019 at 336.895 kb on - strand, within GFF1019 at 336.895 kb on - strand, within GFF1019 at 336.895 kb on - strand, within GFF1019 at 337.254 kb on + strand, within GFF1019 at 337.255 kb on - strand, within GFF1019 at 337.255 kb on - strand, within GFF1019 at 337.255 kb on - strand, within GFF1019 at 337.255 kb on - strand, within GFF1019 at 337.320 kb on + strand, within GFF1019 at 337.320 kb on + strand, within GFF1019 at 337.321 kb on - strand, within GFF1019 at 337.379 kb on + strand, within GFF1019 at 337.380 kb on - strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.413 kb on + strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.414 kb on - strand, within GFF1019 at 337.620 kb on + strand, within GFF1019 at 337.620 kb on + strand, within GFF1019 at 337.621 kb on - strand, within GFF1019 at 337.621 kb on - strand, within GFF1019 at 337.621 kb on - strand, within GFF1019 at 337.646 kb on + strand, within GFF1019 at 337.646 kb on + strand, within GFF1019 at 337.646 kb on + strand, within GFF1019 at 337.646 kb on + strand, within GFF1019
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 335,161 + GFF1017 0.20 +0.2 335,161 + GFF1017 0.20 +0.8 335,162 - GFF1017 0.20 -0.4 335,162 - GFF1017 0.20 +0.2 335,162 - GFF1017 0.20 -0.8 335,240 - GFF1017 0.28 -0.9 335,333 + GFF1017 0.37 +0.0 335,545 + GFF1017 0.59 -1.1 335,545 + GFF1017 0.59 -0.4 335,546 - GFF1017 0.59 -1.3 335,546 - GFF1017 0.59 -1.4 335,546 - GFF1017 0.59 -0.4 335,650 + GFF1017 0.70 -0.9 335,650 + GFF1017 0.70 +0.5 335,650 + GFF1017 0.70 +0.7 335,650 + GFF1017 0.70 -0.2 335,650 + GFF1017 0.70 +0.8 335,651 - GFF1017 0.70 -1.3 335,651 - GFF1017 0.70 -0.2 335,651 - GFF1017 0.70 -0.4 335,651 - GFF1017 0.70 +1.4 335,651 - GFF1017 0.70 -0.1 335,651 - GFF1017 0.70 -0.8 335,651 - GFF1017 0.70 -0.2 335,891 + +0.8 335,892 - +0.5 335,965 - -0.7 335,965 - -0.6 335,965 - -0.8 335,970 + +1.8 335,971 - +0.4 336,042 + -0.2 336,442 + GFF1018 0.62 -0.7 336,442 + GFF1018 0.62 +0.4 336,442 + GFF1018 0.62 -0.7 336,442 + GFF1018 0.62 +0.6 336,442 + GFF1018 0.62 +1.2 336,442 + GFF1018 0.62 -0.2 336,443 - GFF1018 0.62 +0.2 336,443 - GFF1018 0.62 -0.4 336,443 - GFF1018 0.62 -0.2 336,443 - GFF1018 0.62 -0.2 336,443 - GFF1018 0.62 +0.0 336,534 + GFF1018 0.74 +0.1 336,534 + GFF1018 0.74 -1.4 336,534 + GFF1018 0.74 -1.6 336,535 - GFF1018 0.74 +1.8 336,535 - GFF1018 0.74 +0.4 336,535 - GFF1018 0.74 +0.2 336,535 - GFF1018 0.74 -0.2 336,535 - GFF1018 0.74 -0.6 336,535 - GFF1018 0.74 -2.2 336,535 - GFF1018 0.74 -1.2 336,535 - GFF1018 0.74 +1.8 336,538 + GFF1018 0.75 +0.0 336,538 + GFF1018 0.75 +0.7 336,538 + GFF1018 0.75 -0.1 336,538 + GFF1018 0.75 -0.2 336,538 + GFF1018 0.75 +0.9 336,538 + GFF1018 0.75 -0.2 336,538 + GFF1018 0.75 -0.1 336,538 + GFF1018 0.75 -0.3 336,538 + GFF1018 0.75 +0.6 336,539 - GFF1018 0.75 -0.4 336,539 - GFF1018 0.75 -0.6 336,697 + -0.5 336,697 + -0.4 336,697 + -0.6 336,698 - -0.3 336,698 - +0.2 336,735 + +0.6 336,735 + +0.4 336,735 + +1.1 336,736 - -1.5 336,736 - -0.6 336,777 + +1.0 336,777 + +0.0 336,777 + +1.3 336,777 + -0.8 336,777 + -0.6 336,777 + -0.6 336,777 + +0.2 336,778 - -0.8 336,778 - +0.0 336,778 - -1.2 336,778 - -0.5 336,894 + GFF1019 0.16 -0.8 336,895 - GFF1019 0.16 -0.6 336,895 - GFF1019 0.16 +0.4 336,895 - GFF1019 0.16 -0.2 337,254 + GFF1019 0.51 -0.3 337,255 - GFF1019 0.51 +0.3 337,255 - GFF1019 0.51 +2.8 337,255 - GFF1019 0.51 -0.5 337,255 - GFF1019 0.51 +1.6 337,320 + GFF1019 0.57 -0.8 337,320 + GFF1019 0.57 -0.5 337,321 - GFF1019 0.57 +0.4 337,379 + GFF1019 0.63 +0.1 337,380 - GFF1019 0.63 +0.8 337,413 + GFF1019 0.66 -0.1 337,413 + GFF1019 0.66 +0.4 337,413 + GFF1019 0.66 -0.8 337,413 + GFF1019 0.66 -0.8 337,413 + GFF1019 0.66 -0.8 337,414 - GFF1019 0.66 +0.4 337,414 - GFF1019 0.66 +0.1 337,414 - GFF1019 0.66 +0.8 337,414 - GFF1019 0.66 -0.8 337,414 - GFF1019 0.66 -0.6 337,414 - GFF1019 0.66 +0.1 337,414 - GFF1019 0.66 +0.8 337,414 - GFF1019 0.66 -0.4 337,620 + GFF1019 0.86 +0.0 337,620 + GFF1019 0.86 +0.2 337,621 - GFF1019 0.86 -3.3 337,621 - GFF1019 0.86 +0.4 337,621 - GFF1019 0.86 +0.3 337,646 + GFF1019 0.89 -1.7 337,646 + GFF1019 0.89 -1.0 337,646 + GFF1019 0.89 -0.1 337,646 + GFF1019 0.89 -1.6
Or see this region's nucleotide sequence